>HELIUM_000100422_612GNAAXX:7:99:12017:19418#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(C:07)":54,"(A:02)->(G:17)":59,"(C:02)->(G:10)":42},"sample":"29a_F260619","score":567,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcggcagagtactaccggcagt
agcttaaaactcaaaggacttggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:56:19300:10949#0/1_sub[28..127] {"ali_dir":"left","count":37,"experiment":"wolf_diet","merged_sample":{"29a_F260619":37},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"sample":"29a_F260619"}
ttagccctaaacacaagtaattaatataacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:117:10934:7472#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":31,"pairing_fast_overlap":62,"pairing_fast_score":0.525,"pairing_mismatches":{"(a:02)->(g:34)":77,"(a:33)->(g:02)":67,"(c:32)->(a:02)":53,"(g:31)->(c:02)":55,"(t:32)->(g:02)":39,"(t:33)->(g:02)":35},"sample":"29a_F260619","score":120,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatacccgt
>HELIUM_000100422_612GNAAXX:7:28:9432:2506#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ccagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:94:11447:14902#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(G:02)->(C:07)":41},"sample":"15a_F730814","score":1110,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattagtataacaaaattattccccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:21:10217:20418#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(a:34)->(g:02)":32,"(c:32)->(a:32)":68},"sample":"15a_F730814","score":2935,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaamactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:78:9189:5836#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagctttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:45:12199:11223#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(A:28)->(G:10)":52,"(T:08)->(G:34)":56,"(T:34)->(G:13)":21},"sample":"29a_F260619","score":2050,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:31:6024:16312#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(c:02)->(t:32)":81,"(t:29)->(g:07)":35,"(t:33)->(g:18)":71},"sample":"29a_F260619","score":2785,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacccaagtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:46:17940:13153#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agattaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:93:8154:17231#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:02)->(C:33)":54,"(A:02)->(G:08)":52,"(C:02)->(T:31)":65},"sample":"15a_F730814","score":1545,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
gtagccctaaacacaggtaattaatataacaaaattattcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:88:2764:5509#0/1_sub[75..81] {"ali_dir":"right","ali_length":61,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":61,"pairing_fast_score":1,"sample":"13a_F730603","score":4474,"score_norm":1,"seq_a_single":47,"seq_ab_match":61,"seq_b_single":47}
ccattaa
>HELIUM_000100422_612GNAAXX:7:119:1724:13847#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(a:10)->(c:02)":43,"(c:02)->(t:34)":81,"(c:30)->(a:34)":75,"(c:33)->(a:02)":42},"sample":"29a_F260619","score":1703,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgcaagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:84:12767:8250#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(a:33)->(g:02)":29,"(c:08)->(t:02)":48},"sample":"15a_F730814","score":1457,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagcactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgcccgt
>HELIUM_000100422_612GNAAXX:7:95:3047:19883#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":63,"pairing_fast_score":0.65,"pairing_mismatches":{"(c:02)->(t:18)":80,"(c:16)->(a:32)":58,"(c:24)->(a:16)":66,"(t:33)->(g:02)":38},"sample":"26a_F040644","score":1053,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgcaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:25:6267:15592#0/1_sub[28..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":64,"pairing_fast_score":0.803,"pairing_mismatches":{"(A:02)->(C:34)":70,"(C:35)->(A:02)":22,"(T:34)->(G:02)":35},"sample":"26a_F040644","score":155,"score_norm":0.953,"seq_a_single":44,"seq_ab_match":61,"seq_b_single":44}
ttagccctaaacataaacattcaataaacaagaatgtcgccagagtactactagcaacag
cctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:7:15981:17038#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":63,"pairing_fast_score":0.733,"pairing_mismatches":{"(A:02)->(G:09)":51,"(C:02)->(A:34)":58,"(C:02)->(G:08)":56},"sample":"26a_F040644","score":984,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactgctaggaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:44:2961:16008#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(t:33)->(g:16)":48,"(t:34)->(g:09)":71},"sample":"29a_F260619","score":2269,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtagtaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:106:18403:19369#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":63,"pairing_fast_score":0.733,"pairing_mismatches":{"(c:02)->(a:34)":68,"(c:14)->(a:32)":58},"sample":"26a_F040644","score":1422,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcactaaacacgaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:38:8498:12523#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatctct
>HELIUM_000100422_612GNAAXX:7:117:9530:9312#0/1_sub[28..126] {"ali_dir":"left","count":6,"experiment":"wolf_diet","merged_sample":{"26a_F040644":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataaacattcaataaacaaaaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:25:16186:2004#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:114:18096:18719#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":62,"pairing_fast_score":0.593,"pairing_mismatches":{"(A:34)->(G:02)":31,"(C:02)->(A:26)":67,"(C:02)->(G:15)":56,"(C:25)->(G:02)":42},"sample":"29a_F260619","score":51,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcagggggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:36:9557:4409#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":63,"pairing_fast_score":0.983,"pairing_mismatches":{},"sample":"26a_F040644","score":3323,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaataaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:14:4246:13125#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(c:02)->(a:10)":42,"(c:02)->(a:34)":75,"(c:02)->(t:34)":65,"(t:26)->(g:07)":35},"sample":"29a_F260619","score":756,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgacagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:114:16029:18710#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(A:02)->(G:15)":52,"(A:31)->(G:19)":31,"(C:02)->(A:18)":59,"(C:02)->(G:12)":50,"(C:02)->(G:30)":56},"sample":"29a_F260619","score":1361,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccaaagtagtgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:75:7991:6305#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agtttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:39:3600:15589#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(c:28)->(t:28)":51,"(t:34)->(c:08)":38},"sample":"15a_F730814","score":3213,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtacyaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:83:13800:18140#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggctgtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:101:5125:14603#0/1_sub[28..127] {"ali_dir":"left","count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ctagccttaaacacaaatagttatgcaaacaaaaccattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:97:19225:13051#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(A:02)->(T:30)":65,"(A:15)->(G:16)":32,"(A:22)->(G:21)":34,"(C:02)->(A:29)":68,"(C:02)->(G:30)":56},"sample":"15a_F730814","score":931,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacagaattattcgccagagtactaccggcaat
agcttaaaactcaaaggactgggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:63:4595:15643#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"29a_F260619","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:36:7608:2612#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:101:17248:8585#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:02)->(G:15)":49,"(C:02)->(A:09)":43,"(G:28)->(T:02)":25,"(G:34)->(A:02)":20},"sample":"13a_F730603","score":235,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgcaagagtgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:79:18026:13754#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(c:10)->(a:34)":66,"(c:23)->(a:34)":73},"sample":"26a_F040644","score":3430,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaata
acttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:27:17733:18003#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:93:2167:2202#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":63,"pairing_fast_score":0.583,"pairing_mismatches":{"(C:02)->(A:18)":58,"(C:02)->(A:34)":68,"(C:02)->(G:24)":71,"(G:25)->(T:02)":36,"(T:32)->(G:02)":35,"(T:33)->(A:02)":21},"sample":"26a_F040644","score":488,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactgaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:119:18455:5647#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(A:02)->(C:08)":52,"(C:02)->(A:34)":67,"(T:02)->(G:23)":38,"(T:33)->(A:02)":21},"sample":"15a_F730814","score":619,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattagtcgccagagtactcccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:89:19161:1252#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":63,"pairing_fast_score":0.683,"pairing_mismatches":{"(a:32)->(g:02)":33,"(a:34)->(g:02)":30,"(t:32)->(g:02)":37},"sample":"26a_F040644","score":953,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctacacataaacattcactcaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:105:18769:14453#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacgggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:111:16146:19170#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(A:34)->(G:22)":33,"(C:02)->(T:11)":56,"(G:02)->(T:15)":55},"sample":"15a_F730814","score":833,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccttcaat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:2:7990:17026#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":63,"pairing_fast_score":0.7,"pairing_mismatches":{"(a:09)->(c:28)":63,"(a:09)->(g:34)":76,"(t:30)->(g:10)":70,"(t:32)->(g:02)":37},"sample":"26a_F040644","score":1998,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaaatcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:46:15685:11037#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":7,"experiment":"wolf_diet","merged_sample":{"26a_F040644":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacatgaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:77:12954:1647#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":63,"pairing_fast_score":0.733,"pairing_mismatches":{"(a:02)->(g:34)":76,"(a:02)->(t:07)":33,"(c:02)->(a:33)":58,"(t:02)->(c:30)":63},"sample":"26a_F040644","score":374,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaccattcaataaacaagtatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:97:5546:19321#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(t:02)->(c:02)":38},"sample":"13a_F730603","score":27,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactaytcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:88:13532:13863#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacagccct
>HELIUM_000100422_612GNAAXX:7:89:8751:9379#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttcgcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:24:13626:16590#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(A:02)->(C:14)":69,"(G:02)->(C:07)":55},"sample":"13a_F730603","score":1522,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacccgcaat
agcttaaaccccaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:119:3444:13473#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(a:10)->(c:27)":63,"(a:34)->(g:02)":49,"(c:27)->(a:02)":42},"sample":"15a_F730814","score":1460,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactatcggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:69:10691:16179#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(A:34)->(G:21)":28,"(T:02)->(G:10)":70},"sample":"26a_F040644","score":2472,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaacgcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:65:10440:10063#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":3525,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaag
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:14:8170:14553#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:02)->(G:15)":58,"(A:33)->(G:02)":32,"(G:02)->(T:06)":55,"(G:32)->(A:02)":20},"sample":"13a_F730603","score":49,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacctgcgat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:20:14615:20223#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:28)->(G:02)":23,"(G:21)->(A:02)":24,"(G:21)->(T:05)":62,"(T:26)->(G:31)":36},"sample":"15a_F730814","score":1270,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattagtataacaaaatgattcgccagagtactaccggcaat
agcttaaaactcaaagggcttggcggtgcttgataccctt
>HELIUM_000100422_612GNAAXX:7:32:9017:5807#0/1_sub[28..133] {"ali_dir":"left","ali_length":56,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":56,"pairing_fast_score":0.943,"pairing_mismatches":{},"sample":"26a_F040644","score":1803,"score_norm":0.982,"seq_a_single":52,"seq_ab_match":55,"seq_b_single":52}
ttagccctaaacgtaaactgcaaactattccataataaaataattcgccagaggactact
agcaatagcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:42:12452:15035#0/1_sub[28..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":64,"pairing_fast_score":0.934,"pairing_mismatches":{"(g:07)->(t:34)":36},"sample":"26a_F040644","score":3509,"score_norm":0.984,"seq_a_single":44,"seq_ab_match":63,"seq_b_single":44}
ttagccctaaacataacattcaataaacaagaatgttcgccagagtactactagcaacag
cctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:98:10311:2164#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":30,"pairing_fast_overlap":62,"pairing_fast_score":0.508,"pairing_mismatches":{"(c:02)->(a:34)":75,"(c:02)->(g:34)":77,"(c:09)->(a:18)":59,"(c:10)->(a:34)":67,"(c:10)->(t:02)":48,"(c:15)->(a:02)":34,"(g:22)->(t:10)":62},"sample":"15a_F730814","score":577,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaacttattcgccagagcactaccggcaat
agcttaaaactcaaaggacttggcggcgctttataccctt
>HELIUM_000100422_612GNAAXX:7:90:2566:7720#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"29a_F260619","score":2254,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaactcaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:69:2757:20296#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":9,"experiment":"wolf_diet","merged_sample":{"15a_F730814":8,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattactcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:55:11346:7118#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":7,"experiment":"wolf_diet","merged_sample":{"26a_F040644":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactaccagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:105:4873:16466#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(c:26)->(a:34)":66,"(t:25)->(g:08)":80,"(t:30)->(g:07)":36,"(t:30)->(g:09)":38},"sample":"29a_F260619","score":2621,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacccaaggaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:94:2144:1274#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":10,"experiment":"wolf_diet","merged_sample":{"26a_F040644":10},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactcctagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:116:2559:5871#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":27,"pairing_fast_overlap":62,"pairing_fast_score":0.458,"pairing_mismatches":{"(a:33)->(g:02)":33,"(a:34)->(g:19)":58,"(c:32)->(g:02)":42,"(c:34)->(g:15)":53,"(g:02)->(t:27)":81,"(t:02)->(g:18)":80,"(t:31)->(g:02)":39,"(t:33)->(g:02)":35},"sample":"29a_F260619","score":1243,"score_norm":0.806,"seq_a_single":46,"seq_ab_match":50,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agctcaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:111:13675:10948#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"pairing_mismatches":{},"sample":"15a_F730814","score":2035,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacacaagtattaatataacaaaattattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:91:6069:3262#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"26a_F040644","score":2374,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtacaactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:6:14697:16535#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(A:02)->(C:33)":54,"(A:02)->(T:06)":52,"(G:02)->(A:10)":56},"sample":"29a_F260619","score":1465,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtacttccgacaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:92:9110:15028#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatacaacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:39:3907:17871#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtagtaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:8466:2855#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(a:07)->(g:33)":77,"(a:10)->(t:02)":39,"(c:10)->(t:02)":48},"sample":"15a_F730814","score":1633,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatacgccagagcactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:108:5424:15440#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":63,"pairing_fast_score":0.883,"pairing_mismatches":{"(t:33)->(c:02)":37},"sample":"26a_F040644","score":2141,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataagctattttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:103:6760:11100#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":1635,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatgtccct
>HELIUM_000100422_612GNAAXX:7:37:11872:8333#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:02)->(C:22)":72,"(A:10)->(C:23)":54,"(G:02)->(A:12)":62},"sample":"29a_F260619","score":1319,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttacccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
aacttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:37:9130:3330#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(C:02)->(G:24)":56},"sample":"29a_F260619","score":1873,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattattatatcaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:67:17700:4725#0/1_sub[28..126] {"ali_dir":"left","count":438,"experiment":"wolf_diet","merged_sample":{"26a_F040644":438},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:7:8807:7823#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtcataccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:22:1708:19102#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaatcataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:24:18529:5051#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(t:29)->(a:10)":35},"sample":"29a_F260619","score":3656,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactactagcaac
agattaaacctcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:51:10241:16284#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctacacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:60:15373:16200#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"sample":"15a_F730814","score":1902,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagcaattaatataacaaaattattcgccagagtactactggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:111:10417:15895#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":31,"pairing_fast_overlap":62,"pairing_fast_score":0.525,"pairing_mismatches":{"(A:14)->(G:02)":34,"(A:22)->(G:16)":41,"(C:02)->(G:10)":50,"(C:02)->(G:11)":54,"(C:34)->(G:02)":26,"(T:21)->(G:29)":39,"(T:34)->(A:02)":21},"sample":"29a_F260619","score":405,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgtgcaccagagtagtaggggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:5:11597:9443#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(c:02)->(t:34)":81,"(c:07)->(g:02)":45,"(c:10)->(t:02)":39,"(c:22)->(g:27)":56,"(c:28)->(a:02)":42,"(t:29)->(a:02)":35},"sample":"15a_F730814","score":107,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatccgccacagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:88:3210:19068#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":32,"pairing_fast_overlap":62,"pairing_fast_score":0.542,"pairing_mismatches":{"(a:02)->(c:31)":72,"(a:02)->(g:34)":77,"(a:06)->(c:02)":50,"(a:08)->(t:02)":48,"(g:02)->(t:34)":81,"(t:33)->(a:02)":35},"sample":"29a_F260619","score":97,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccaaagaaataccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:23:2588:18862#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":3647,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaataattatgcaaacaaaactattcgtcagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:23:9562:6073#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(T:07)->(A:07)":32},"sample":"15a_F730814","score":1451,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaacacaagtaattaatataacaawattattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:113:15796:3810#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(A:02)->(G:09)":52,"(G:02)->(T:15)":55},"sample":"15a_F730814","score":1501,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgcctgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:36:2707:12279#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":13,"experiment":"wolf_diet","merged_sample":{"15a_F730814":5,"29a_F260619":8},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaataattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:76:16217:13507#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(A:02)->(G:11)":52,"(T:33)->(G:02)":21},"sample":"29a_F260619","score":547,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
tcagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:74:14920:18453#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":63,"pairing_fast_score":0.65,"pairing_mismatches":{"(A:02)->(G:18)":60,"(A:31)->(G:02)":33,"(A:32)->(G:02)":28,"(C:02)->(A:23)":58,"(C:02)->(G:08)":62,"(G:33)->(C:02)":32},"sample":"26a_F040644","score":657,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacg
ggctgaaactcaaaggacttggcggtgctttacagccct
>HELIUM_000100422_612GNAAXX:7:92:6909:16766#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(G:02)->(A:10)":56,"(T:18)->(G:02)":35},"sample":"15a_F730814","score":155,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccgacaat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:110:9830:19958#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":63,"pairing_fast_score":0.667,"pairing_mismatches":{"(T:18)->(G:08)":37,"(T:27)->(G:02)":26,"(T:34)->(G:02)":21},"sample":"26a_F040644","score":665,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataagctattctataacaaaataattcgccagagaactactagcaaca
gcttaaaactcaaaggacgtggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:101:9416:17292#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"pairing_mismatches":{},"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggatttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:89:12401:1946#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcacacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:103:16326:19255#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":63,"pairing_fast_score":0.65,"pairing_mismatches":{"(A:28)->(G:02)":48,"(A:34)->(G:02)":27,"(C:34)->(G:02)":29,"(T:22)->(G:02)":50,"(T:31)->(G:02)":38},"sample":"26a_F040644","score":619,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaagggctgggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:6:17517:5352#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":10,"experiment":"wolf_diet","merged_sample":{"26a_F040644":10},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcaccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:34:1841:5593#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3785,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaataagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:108:19171:11413#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtagtcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:29:18899:11661#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"13a_F730603":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaagactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:44:5008:2115#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"sample":"26a_F040644","score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:65:9852:8150#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(A:02)->(G:06)":51,"(A:02)->(T:10)":48},"sample":"26a_F040644","score":664,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagttctgctagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:116:3564:16863#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(a:02)->(c:34)":70,"(a:10)->(c:02)":40,"(c:08)->(a:02)":42,"(t:13)->(c:02)":43,"(t:34)->(c:02)":38},"sample":"29a_F260619","score":412,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattagctagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:72:15757:20860#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":63,"pairing_fast_score":0.683,"pairing_mismatches":{"(C:31)->(G:02)":39,"(C:34)->(A:02)":29,"(C:34)->(G:02)":23,"(T:33)->(G:02)":21,"(T:34)->(G:02)":26},"sample":"29a_F260619","score":1318,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactactagcaaca
gattaaacctcaaaggacttggcagtgctttatacccat
>HELIUM_000100422_612GNAAXX:7:57:11518:7009#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(c:09)->(g:34)":76},"sample":"26a_F040644","score":4041,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaacaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:33:6831:16430#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":3678,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgtcagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:93:7213:12063#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"sample":"13a_F730603","score":1337,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaatctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:90:17862:20267#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:02)->(G:06)":52,"(T:23)->(G:21)":36,"(T:34)->(G:09)":27},"sample":"15a_F730814","score":943,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagggtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:111:19232:18516#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(A:02)->(C:25)":54,"(A:02)->(G:08)":49,"(A:02)->(G:19)":58,"(A:26)->(G:02)":28,"(A:34)->(T:02)":23,"(T:34)->(G:02)":21},"sample":"15a_F730814","score":206,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcgat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:114:16286:20301#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(a:02)->(t:32)":81,"(a:20)->(g:34)":77,"(c:15)->(a:33)":68,"(c:18)->(g:17)":56,"(t:18)->(g:18)":71,"(t:29)->(g:18)":39,"(t:32)->(g:23)":80},"sample":"29a_F260619","score":2041,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccgccaat
agcttaaaackcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:12463:12890#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(T:02)->(G:18)":51},"sample":"29a_F260619","score":1541,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtacgaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:116:16273:14885#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(C:02)->(A:28)":59,"(G:02)->(A:17)":55},"sample":"29a_F260619","score":842,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccagcaat
agcttaaaactcaaagaacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:72:8599:12369#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":62,"pairing_fast_score":0.983,"sample":"13a_F730603","score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtattaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:16:15384:11538#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(A:02)->(G:20)":48,"(C:26)->(G:30)":22},"sample":"26a_F040644","score":1354,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattgaataaacaagaatgttcgccagagtgctactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:53:2210:4216#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(C:34)->(A:16)":22},"sample":"26a_F040644","score":2981,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcaccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:5:8956:15004#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":9,"experiment":"wolf_diet","merged_sample":{"26a_F040644":9},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcacaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:60:6552:14786#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtagtaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:74:13981:19857#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":12,"experiment":"wolf_diet","merged_sample":{"15a_F730814":6,"29a_F260619":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccgtcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:114:16757:5672#0/1_sub[28..126] {"ali_dir":"left","count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactaacaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:114:17228:5028#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"13a_F730603":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttacgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:98:16722:9236#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagtgtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:52:15242:3400#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggactcggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:109:12225:15546#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":31,"pairing_fast_overlap":62,"pairing_fast_score":0.525,"pairing_mismatches":{"(A:02)->(C:29)":54,"(A:02)->(T:10)":49,"(A:11)->(T:31)":39,"(A:20)->(T:25)":36,"(C:02)->(T:29)":65,"(T:02)->(G:22)":71},"sample":"29a_F260619","score":1232,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagttctaccggcaat
agcttaaaacgcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:73:18540:5495#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(C:31)->(A:28)":32,"(C:34)->(G:11)":42,"(T:02)->(G:17)":71,"(T:33)->(G:06)":38},"sample":"29a_F260619","score":2038,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacacaattattcgccagagtactaccggcaat
agcttaaaacgcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:17:8191:8188#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":3636,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaaataattatataaacaaaattattcgccagagtactaccggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:97:12182:5973#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(c:02)->(a:34)":67,"(g:02)->(c:34)":79,"(t:02)->(c:34)":70},"sample":"29a_F260619","score":421,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagggtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:60:4661:7690#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(c:09)->(a:33)":67},"sample":"26a_F040644","score":3672,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgcttaacatccct
>HELIUM_000100422_612GNAAXX:7:5:2541:4295#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:02)->(G:07)":69,"(A:02)->(T:10)":52},"sample":"13a_F730603","score":16,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtacttccggcaat
agcttaaagctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:2:16632:14791#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(a:02)->(g:32)":76,"(a:11)->(t:02)":47},"sample":"26a_F040644","score":122,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagaactactaacaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:99:17203:12481#0/1_sub[71..81] {"ali_dir":"right","ali_length":65,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":62,"pairing_fast_overlap":65,"pairing_fast_score":1,"sample":"29a_F260619","score":4704,"score_norm":1,"seq_a_single":43,"seq_ab_match":65,"seq_b_single":43}
tttataccctt
>HELIUM_000100422_612GNAAXX:7:48:19400:1582#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":16,"experiment":"wolf_diet","merged_sample":{"13a_F730603":16},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaagctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:49:4742:4749#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"29a_F260619","score":3519,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcagaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:94:5155:17377#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"pairing_mismatches":{"(T:30)->(A:02)":21,"(T:32)->(G:32)":38},"sample":"29a_F260619","score":1235,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattaktcgccagagtactaccggcaat
agcttaaaactcaaaggactgggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:101:16567:21285#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":6,"experiment":"wolf_diet","merged_sample":{"26a_F040644":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaggaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:112:7603:11308#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttagcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:28:7386:18761#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(a:07)->(c:02)":42,"(c:02)->(t:34)":81,"(c:09)->(g:02)":45,"(t:11)->(a:02)":35},"sample":"15a_F730814","score":1625,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgacacagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:61:19148:17461#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacgtggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:40:7630:14326#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(a:14)->(c:02)":43,"(a:31)->(g:02)":31},"sample":"29a_F260619","score":11,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgcaagggtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:67:1659:20068#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agctaaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:10:3024:17714#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"29a_F260619","score":1443,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaagctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:10:5206:12276#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcttaaaactcaaaggacttggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:71:6711:16978#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(A:02)->(C:32)":70,"(A:02)->(G:09)":49,"(C:02)->(A:29)":69,"(C:02)->(G:31)":56,"(C:02)->(T:34)":65},"sample":"29a_F260619","score":1184,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatatagcaaaattattcgccagagtgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:83:13079:6138#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:02)->(C:34)":54,"(T:31)->(G:31)":38},"sample":"15a_F730814","score":890,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattaktcgccagagtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:57:13000:20853#0/1_sub[28..127] {"ali_dir":"left","count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603","score_norm":0.968}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
aacttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:119:17651:19784#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:02)->(G:09)":52,"(C:02)->(A:33)":59,"(G:34)->(A:02)":20,"(T:32)->(G:02)":21},"sample":"13a_F730603","score":974,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctgccggcaat
agcttaaaactcaaaggacttggcagtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:78:12575:8285#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"13a_F730603":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaataattatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:54:9665:3264#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(T:34)->(A:02)":25},"sample":"29a_F260619","score":820,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcaccagagtactagcggcaaa
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:118:16523:13979#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{},"sample":"13a_F730603","score":1060,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgaaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:68:19236:20339#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":22,"pairing_fast_overlap":63,"pairing_fast_score":0.367,"pairing_mismatches":{"(a:23)->(g:02)":48,"(c:02)->(a:26)":67,"(c:02)->(a:28)":77,"(c:07)->(a:25)":58,"(c:10)->(a:02)":51,"(c:16)->(g:02)":46,"(c:18)->(g:02)":41,"(c:34)->(g:02)":29,"(t:19)->(g:02)":50,"(t:28)->(g:02)":38},"sample":"26a_F040644","score":349,"score_norm":0.81,"seq_a_single":45,"seq_ab_match":51,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagactactcctagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:17:1743:13061#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":3078,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctgtataccctt
>HELIUM_000100422_612GNAAXX:7:6:16738:6753#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":11,"experiment":"wolf_diet","merged_sample":{"15a_F730814":8,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccccaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:16981:17584#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":8,"experiment":"wolf_diet","merged_sample":{"15a_F730814":5,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtacttccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:53:16956:10563#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"sample":"26a_F040644","score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaaggatgttcgcaagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:54:10323:7022#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaaataattatataaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:118:6881:20318#0/1_sub[28..126] {"ali_dir":"left","count":7,"experiment":"wolf_diet","merged_sample":{"26a_F040644":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacctggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:106:11034:12809#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{},"sample":"26a_F040644","score":8,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaagractyggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:5:10488:21032#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":63,"pairing_fast_score":0.617,"pairing_mismatches":{"(a:02)->(c:32)":71,"(c:02)->(a:32)":58,"(c:02)->(g:15)":76,"(c:34)->(g:02)":41,"(t:02)->(c:34)":59},"sample":"26a_F040644","score":21,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgttgtacatccct
>HELIUM_000100422_612GNAAXX:7:99:17543:14412#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":63,"pairing_fast_score":0.717,"pairing_mismatches":{"(a:02)->(c:34)":69,"(c:02)->(t:32)":80,"(t:33)->(g:17)":37},"sample":"29a_F260619","score":1931,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataagctattccctaacaaaataattcgccagagaactactagcaaca
gattaaacctcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:75:14707:15470#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(A:32)->(G:02)":28,"(C:12)->(A:34)":58},"sample":"26a_F040644","score":1708,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaacttaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:105:15700:19259#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcamca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:53:19272:10781#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(c:02)->(a:32)":74,"(c:02)->(t:34)":65,"(c:31)->(a:18)":42,"(t:02)->(a:34)":67},"sample":"29a_F260619","score":835,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacacgtaatcagtataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:84:13091:9531#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"15a_F730814":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagcgtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:48:13158:13053#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaatatacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:90:2671:8885#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{},"sample":"13a_F730603","score":3325,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactctaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:22:5492:19108#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":63,"pairing_fast_score":0.683,"pairing_mismatches":{"(A:02)->(C:11)":63,"(A:02)->(T:12)":68,"(G:32)->(C:02)":32,"(T:18)->(A:02)":37},"sample":"26a_F040644","score":183,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaatctcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:94:7885:15812#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcgaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:106:8356:11465#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(a:10)->(c:27)":54,"(a:16)->(c:14)":72,"(a:34)->(g:09)":68},"sample":"15a_F730814","score":2014,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactaaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:114:8508:6241#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagtaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:85:11084:18047#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:32)->(G:02)":33,"(C:02)->(T:34)":65,"(G:02)->(A:10)":56,"(T:23)->(G:34)":38},"sample":"29a_F260619","score":544,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattagtcgccagagtactaccgacaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:114:11698:17912#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(G:02)->(T:17)":62,"(G:02)->(T:20)":55},"sample":"29a_F260619","score":1559,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaat
atcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:19:9558:13618#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"13a_F730603","score":3629,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ctagccttaaacacaaatagtatgcaaacaaaactattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:78:13477:20631#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":62,"pairing_fast_score":0.593,"pairing_mismatches":{"(A:02)->(G:17)":34,"(T:02)->(G:09)":51},"sample":"13a_F730603","score":707,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaagctattcgccagagtacgaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:89:4146:14622#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(C:34)->(A:02)":22,"(T:32)->(G:02)":20},"sample":"26a_F040644","score":1119,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttatccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:7:2587:16782#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":63,"pairing_fast_score":0.917,"pairing_mismatches":{"(t:05)->(c:02)":49},"sample":"26a_F040644","score":847,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggattactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:102:1765:7063#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(A:02)->(C:09)":43,"(A:02)->(T:07)":49,"(A:02)->(T:10)":52,"(A:02)->(T:27)":65,"(C:02)->(G:12)":56,"(G:02)->(T:06)":41},"sample":"15a_F730814","score":1070,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattctccagagttcttccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:37:17937:5943#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"sample":"15a_F730814","score":1138,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagggtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:25:8147:9392#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":3905,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcgaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:53:18959:6744#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(a:19)->(g:02)":33,"(c:10)->(t:02)":47,"(t:16)->(g:02)":38},"sample":"26a_F040644","score":417,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagcactactagcaaca
gcctgaaacttaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:28:5876:14810#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaattcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:32:3292:3549#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgctcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:101:7576:13399#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":27,"pairing_fast_overlap":62,"pairing_fast_score":0.458,"pairing_mismatches":{"(A:02)->(C:24)":54,"(A:02)->(G:09)":58,"(A:02)->(T:23)":36,"(A:32)->(G:22)":23,"(C:02)->(A:32)":67,"(G:02)->(T:10)":62,"(T:02)->(G:08)":35},"sample":"15a_F730814","score":425,"score_norm":0.839,"seq_a_single":46,"seq_ab_match":52,"seq_b_single":46}
tcagccctaaacacaagtaattaatataacaaaagtattcgccagagtactaccggcgat
atcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:99:9326:15438#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":63,"pairing_fast_score":0.667,"pairing_mismatches":{"(c:02)->(a:18)":72,"(c:02)->(g:31)":76,"(c:10)->(t:02)":47,"(c:33)->(a:02)":31,"(t:30)->(g:02)":37},"sample":"26a_F040644","score":395,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacacgaatgttcgccagagcactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacgtccct
>HELIUM_000100422_612GNAAXX:7:28:15433:10775#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"sample":"13a_F730603","score":1754,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttgccggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:78:4025:3710#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"sample":"15a_F730814","score":2138,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaacttattcgccagagtactatcggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:119:19479:18233#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(a:02)->(c:21)":54,"(a:02)->(c:23)":43,"(a:02)->(t:25)":81,"(c:02)->(a:16)":42,"(g:02)->(t:14)":76,"(t:02)->(c:06)":38,"(t:02)->(g:15)":36},"sample":"15a_F730814","score":505,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaaataatataacaaaatgactcgacagagtactaccggcaat
agcttaaaactcaaatgacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:52:7798:14057#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacacgtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:94:10708:17117#0/1_sub[28..126] {"ali_dir":"left","count":23,"experiment":"wolf_diet","merged_sample":{"26a_F040644":23},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactggcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:32:13182:8783#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:23:13108:20586#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":7,"experiment":"wolf_diet","merged_sample":{"26a_F040644":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
ggctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:17:4197:19071#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggactgggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:115:14305:13086#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(a:31)->(g:18)":74,"(a:33)->(g:02)":31,"(c:31)->(g:02)":43,"(g:34)->(t:13)":76},"sample":"29a_F260619","score":2031,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgcgttataccctt
>HELIUM_000100422_612GNAAXX:7:98:2178:10959#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(A:34)->(G:02)":28,"(C:34)->(G:02)":30},"sample":"15a_F730814","score":300,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
tgagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:16362:6522#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{"(g:06)->(c:02)":49},"sample":"26a_F040644","score":1444,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccttaaacataaacattcaataaacaagaatgttcgccagagtagtactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:20:11877:16148#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggactttgcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:18:8755:16353#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(T:34)->(G:02)":21},"sample":"29a_F260619","score":1250,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:21:8917:3029#0/1_sub[28..127] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(T:02)->(G:09)":38},"sample":"15a_F730814","score":1170,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacacaagtaattaatataacaaaattagtcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccttt
>HELIUM_000100422_612GNAAXX:7:74:12038:18094#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(C:35)->(A:34)":32},"sample":"15a_F730814","score":2848,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacacaattattcgccagagtactaccggcaat
agcttaaagctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:99:2910:8013#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccgccaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:116:15153:5490#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":63,"pairing_fast_score":0.717,"pairing_mismatches":{"(g:13)->(t:02)":38,"(g:18)->(c:02)":42,"(t:11)->(c:21)":63,"(t:24)->(g:02)":37,"(t:26)->(a:02)":35},"sample":"26a_F040644","score":1082,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtgcgcgagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:93:2024:7489#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(a:10)->(t:02)":48,"(a:11)->(g:15)":77,"(c:09)->(a:02)":46},"sample":"13a_F730603","score":615,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgtcagcgaactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:119:10551:16970#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":63,"pairing_fast_score":0.7,"pairing_mismatches":{"(a:02)->(g:34)":76,"(a:28)->(g:02)":30,"(c:02)->(t:32)":80,"(c:15)->(t:02)":47},"sample":"26a_F040644","score":783,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagcactactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:7:17822:11589#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":63,"pairing_fast_score":0.817,"pairing_mismatches":{"(T:34)->(G:02)":20},"sample":"26a_F040644","score":1125,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcgctgctttacatccct
>HELIUM_000100422_612GNAAXX:7:91:13260:21135#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":34,"experiment":"wolf_diet","merged_sample":{"26a_F040644":34},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacgagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:116:19145:18327#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":63,"pairing_fast_score":0.633,"pairing_mismatches":{"(c:13)->(a:31)":58,"(c:22)->(g:09)":41,"(c:23)->(a:25)":67,"(t:02)->(g:11)":70,"(t:30)->(g:09)":37},"sample":"26a_F040644","score":1720,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacatccaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaacgcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:65:19422:14244#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":30,"pairing_fast_overlap":62,"pairing_fast_score":0.508,"pairing_mismatches":{"(A:02)->(C:30)":54,"(A:18)->(G:02)":23,"(A:30)->(G:02)":37,"(A:34)->(G:02)":29,"(C:02)->(A:20)":68,"(C:02)->(A:23)":59,"(T:22)->(G:02)":35,"(T:31)->(G:02)":27},"sample":"29a_F260619","score":100,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
tcagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:26:18506:2617#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":10,"experiment":"wolf_diet","merged_sample":{"15a_F730814":5,"29a_F260619":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtagttaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:45:7460:13396#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgcccgt
>HELIUM_000100422_612GNAAXX:7:19:7212:6085#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:36:4129:2396#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":63,"pairing_fast_score":0.7,"pairing_mismatches":{"(C:02)->(A:32)":68,"(G:13)->(A:13)":40,"(G:34)->(A:08)":32},"sample":"26a_F040644","score":1459,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcrccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:30:13284:3769#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacattcct
>HELIUM_000100422_612GNAAXX:7:63:5615:2126#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(A:02)->(T:34)":48,"(C:02)->(G:09)":56,"(C:08)->(A:02)":24,"(T:02)->(G:25)":36},"sample":"29a_F260619","score":36,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattactataacaaaatgattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:84:5044:14693#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"15a_F730814","score":1864,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacgaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccgtt
>HELIUM_000100422_612GNAAXX:7:50:11390:9726#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":63,"pairing_fast_score":0.883,"pairing_mismatches":{"(a:13)->(c:34)":69},"sample":"29a_F260619","score":3447,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacctaagctattccataacaaaataattcgccagagaactactagcaaca
gattaaacctcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:16:6796:4484#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(T:35)->(A:02)":27},"sample":"29a_F260619","score":944,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgttttataccctt
>HELIUM_000100422_612GNAAXX:7:58:8409:9911#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaaaataattcgccagaggactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:69:18676:20487#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":63,"pairing_fast_score":0.75,"pairing_mismatches":{"(a:34)->(g:02)":34,"(c:02)->(g:31)":81,"(c:20)->(g:02)":37,"(c:32)->(g:02)":41},"sample":"26a_F040644","score":1655,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgctcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:12:13530:11042#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattatttgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:21:6564:20803#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattacacaaacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:110:10477:17117#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":63,"pairing_fast_score":0.633,"pairing_mismatches":{"(C:02)->(A:28)":58,"(C:02)->(G:09)":56,"(G:02)->(T:11)":55,"(G:30)->(T:02)":36,"(T:25)->(G:02)":25},"sample":"26a_F040644","score":282,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttaaccctaaacataaacattcaataaacaagaatgttcgccagagtactactatgaaca
gcctgaaactcaaaggacttggcggtgctttacagccct
>HELIUM_000100422_612GNAAXX:7:99:18685:1473#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":31,"pairing_fast_overlap":63,"pairing_fast_score":0.517,"pairing_mismatches":{"(A:02)->(G:23)":48,"(A:33)->(G:02)":30,"(C:33)->(A:02)":29,"(T:02)->(G:15)":53,"(T:33)->(G:02)":25},"sample":"26a_F040644","score":53,"score_norm":0.857,"seq_a_single":45,"seq_ab_match":54,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgctacgagcaaca
gcctgaaactcaaaggactgggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:118:3572:14633#0/1_sub[28..126] {"ali_dir":"left","count":10172,"experiment":"wolf_diet","merged_sample":{"26a_F040644":10172},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:1:14046:18929#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaatcattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:85:17693:19474#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":63,"pairing_fast_score":0.7,"pairing_mismatches":{"(a:24)->(g:02)":48,"(a:31)->(g:02)":33},"sample":"26a_F040644","score":285,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccccaaacataaacattcaataaacaagaatgttcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:88:6504:19781#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagaaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:100:18844:15930#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaagcgcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:21:9767:5881#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(T:02)->(A:06)":35},"sample":"15a_F730814","score":880,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaaatattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcgtggctttataccctt
>HELIUM_000100422_612GNAAXX:7:89:18385:1726#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":11,"experiment":"wolf_diet","merged_sample":{"26a_F040644":11},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacg
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:91:13609:20374#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(c:02)->(a:32)":74,"(c:02)->(g:34)":77,"(c:02)->(t:31)":81,"(c:15)->(t:32)":65,"(c:22)->(a:34)":67,"(c:26)->(g:28)":56,"(t:10)->(g:10)":38},"sample":"13a_F730603","score":2197,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactaktcgccagagtactaccggtaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:80:12265:18427#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacaccagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:52:13315:9471#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"pairing_mismatches":{"(A:02)->(G:13)":52,"(A:02)->(T:08)":49},"sample":"29a_F260619","score":1171,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagttctgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:54:4705:14248#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":63,"pairing_fast_score":0.883,"pairing_mismatches":{"(c:02)->(t:34)":80},"sample":"29a_F260619","score":3162,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcaccagagtactagcggcaaca
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:76:9558:8346#0/1_sub[28..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":61,"pairing_fast_overlap":64,"pairing_fast_score":1,"sample":"26a_F040644","score":3287,"score_norm":1,"seq_a_single":44,"seq_ab_match":64,"seq_b_single":44}
ttagccctaacataaacattcaataaacaagaacgttcgccagagtactactagcaacag
cctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:112:15363:15348#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":63,"pairing_fast_score":0.733,"pairing_mismatches":{"(A:02)->(T:09)":51,"(C:02)->(T:34)":64,"(G:09)->(C:02)":19},"sample":"26a_F040644","score":1388,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaatgagtctataacaaaataattcgccagagaacttctagcaaca
gcttaaaactcaaaggacgtggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:67:1874:18443#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(C:02)->(A:29)":59,"(C:02)->(G:33)":56},"sample":"29a_F260619","score":20,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactgccggcaat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:115:15752:7166#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataccaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:112:2074:4844#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":63,"pairing_fast_score":0.733,"pairing_mismatches":{"(a:09)->(c:02)":42,"(a:12)->(c:02)":39,"(g:07)->(a:25)":74},"sample":"26a_F040644","score":1357,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttagcaagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:39:7485:15141#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(a:02)->(c:34)":72,"(c:10)->(g:31)":62,"(t:02)->(g:34)":77},"sample":"29a_F260619","score":2214,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:62:16825:18595#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":6,"experiment":"wolf_diet","merged_sample":{"26a_F040644":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcggcagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(C:02)->(G:06)":54,"(C:02)->(G:15)":56,"(G:02)->(C:05)":55},"sample":"15a_F730814","score":1813,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacgcgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:39:4698:18035#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":8,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3,"29a_F260619":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcagtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:115:12007:14250#0/1_sub[28..127] {"ali_dir":"left","count":6,"experiment":"wolf_diet","merged_sample":{"13a_F730603":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacccgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:29:9723:20435#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:43:4660:16319#0/1_sub[28..126] {"ali_dir":"left","count":22,"experiment":"wolf_diet","merged_sample":{"26a_F040644":22},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:87:4652:13322#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaaatattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:120:4849:10395#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":63,"pairing_fast_score":0.7,"pairing_mismatches":{"(a:34)->(g:02)":30,"(c:06)->(t:02)":47,"(c:11)->(a:02)":51,"(c:21)->(g:02)":41},"sample":"26a_F040644","score":1636,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagcactcctagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:77:9121:9222#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":18,"experiment":"wolf_diet","merged_sample":{"13a_F730603":18},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacctgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:89:17444:19076#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":63,"pairing_fast_score":0.683,"pairing_mismatches":{"(A:02)->(G:10)":51,"(A:34)->(G:18)":48,"(G:02)->(T:14)":61,"(G:28)->(T:09)":36},"sample":"26a_F040644","score":1451,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactgctagcaaca
tcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:15:1413:19057#0/1_sub[28..126] {"ali_dir":"left","count":7,"experiment":"wolf_diet","merged_sample":{"26a_F040644":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttgacggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:57:17029:7235#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{},"sample":"29a_F260619","score":2595,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctatataccctt
>HELIUM_000100422_612GNAAXX:7:93:6036:5117#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":6,"experiment":"wolf_diet","merged_sample":{"26a_F040644":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaagcataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:65:6123:1270#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":63,"pairing_fast_score":0.633,"pairing_mismatches":{"(A:02)->(G:20)":48,"(C:02)->(A:30)":68,"(C:02)->(A:33)":58,"(T:32)->(G:21)":37,"(T:34)->(G:02)":20},"sample":"26a_F040644","score":341,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgctactagcaaca
gcctgaaactcaaagggcttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:50:11845:20849#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(A:29)->(G:09)":34},"sample":"13a_F730603","score":1724,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttaaaccctt
>HELIUM_000100422_612GNAAXX:7:82:4129:17516#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(G:32)->(A:06)":36,"(T:31)->(C:06)":37},"sample":"26a_F040644","score":2594,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagcgctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:38:19686:10444#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":63,"pairing_fast_score":0.667,"pairing_mismatches":{"(a:02)->(c:31)":62,"(a:13)->(c:02)":42,"(a:32)->(g:02)":34,"(t:29)->(g:02)":38},"sample":"26a_F040644","score":169,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaaca
gcctgaaactcaaaggacgtggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:26:4827:20071#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":63,"pairing_fast_score":0.817,"pairing_mismatches":{"(T:02)->(G:11)":50,"(T:02)->(G:13)":53},"sample":"26a_F040644","score":1145,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtacgacgagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:44:18030:16677#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:02)->(T:15)":36,"(C:02)->(A:28)":69,"(C:02)->(A:32)":59},"sample":"29a_F260619","score":650,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaataaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:52:8318:6365#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{},"sample":"29a_F260619","score":3876,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctacacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:83:11368:19988#0/1_sub[27..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":64,"pairing_fast_score":0.77,"pairing_mismatches":{"(T:22)->(C:10)":37,"(T:34)->(G:02)":21},"sample":"26a_F040644","score":616,"score_norm":0.938,"seq_a_single":44,"seq_ab_match":60,"seq_b_single":44}
ttagccctaaacataagctattctataacaaaataattcgccagagaactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:15:12401:9313#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":63,"pairing_fast_score":0.983,"pairing_mismatches":{},"sample":"26a_F040644","score":1307,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ctagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:71:16381:11611#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":63,"pairing_fast_score":0.783,"pairing_mismatches":{"(C:02)->(G:14)":56},"sample":"26a_F040644","score":1375,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactaggaaca
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:77:15920:2671#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":4259,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacacatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:93:16341:14122#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":11,"experiment":"wolf_diet","merged_sample":{"15a_F730814":9,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtacgaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:118:16607:4616#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(a:33)->(g:02)":30,"(t:34)->(g:02)":37},"sample":"26a_F040644","score":1523,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttagccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:59:5028:19734#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcagtaaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:89:14597:8525#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
gtagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:19:17615:7657#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":4115,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatcctt
>HELIUM_000100422_612GNAAXX:7:45:14752:16515#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":4076,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatcctt
>HELIUM_000100422_612GNAAXX:7:24:2383:19423#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":18,"experiment":"wolf_diet","merged_sample":{"26a_F040644":18},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcagca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:84:12730:2140#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgacactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:117:7125:4555#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"13a_F730603":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacagatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:116:14765:19437#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":27,"pairing_fast_overlap":63,"pairing_fast_score":0.45,"pairing_mismatches":{"(a:02)->(g:11)":48,"(a:02)->(g:34)":76,"(a:31)->(g:02)":33,"(a:34)->(g:02)":30,"(c:02)->(a:16)":51,"(c:02)->(a:34)":58,"(c:02)->(g:34)":61,"(c:08)->(a:02)":31,"(t:29)->(g:02)":37},"sample":"26a_F040644","score":405,"score_norm":0.841,"seq_a_single":45,"seq_ab_match":53,"seq_b_single":45}
ttagccctaaacataaacattcaataaacacgaatgttcgccagagtgctactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:103:11045:3860#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaagggcttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:97:11534:9604#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{},"sample":"26a_F040644","score":1964,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaatcagtctataacaaaataattcgccagagaactactagcaaca
gcttaaaactcgaaggacgtggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:77:14768:5692#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"pairing_mismatches":{},"sample":"26a_F040644","score":1333,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttgtcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:120:2027:9746#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":63,"pairing_fast_score":0.65,"pairing_mismatches":{"(A:02)->(C:29)":69,"(C:02)->(G:07)":56,"(C:16)->(A:02)":31,"(C:29)->(T:02)":23},"sample":"29a_F260619","score":448,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacctaagctattccataacacaataattcgccagagaactactaggaaca
gattaaacctcaaaggacttggcagtgctttatgcccct
>HELIUM_000100422_612GNAAXX:7:15:6809:12174#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(A:02)->(G:08)":52,"(C:02)->(G:10)":53,"(C:02)->(G:15)":56,"(G:02)->(T:10)":55,"(T:02)->(G:07)":48,"(T:32)->(G:02)":21},"sample":"29a_F260619","score":1257,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
tcagccctaaacacaagtaattaatataacaaaattattcgccagaggactggctgcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:30:19369:8308#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(A:19)->(C:02)":54,"(A:26)->(C:02)":52,"(A:34)->(G:02)":29,"(C:34)->(A:02)":30,"(T:02)->(G:22)":71},"sample":"29a_F260619","score":295,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacaggcaat
agcttaaaacgcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:47:11226:11375#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"13a_F730603","score":4119,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaatcattctataacaaaataattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:24:11418:10531#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"26a_F040644","score":2111,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaatcattctataacaaaataattcgccggagaactactagcaaca
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:63:4535:4839#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"29a_F260619","score":1405,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:20:16647:20011#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":63,"pairing_fast_score":0.617,"pairing_mismatches":{"(a:10)->(c:02)":39,"(c:02)->(a:34)":66,"(c:24)->(a:34)":58},"sample":"26a_F040644","score":1121,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattagccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:116:8759:16023#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(A:02)->(G:21)":52,"(C:02)->(A:29)":59,"(T:02)->(G:15)":36,"(T:30)->(G:08)":30,"(T:33)->(G:02)":21},"sample":"15a_F730814","score":350,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataataaaatgattcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:95:6820:1925#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":63,"pairing_fast_score":0.6,"pairing_mismatches":{"(C:02)->(A:23)":58,"(C:02)->(G:19)":49,"(G:02)->(A:12)":46,"(T:34)->(G:02)":25},"sample":"26a_F040644","score":805,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagaatagtactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:76:19008:9746#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(A:02)->(G:02)":30,"(T:02)->(C:27)":63},"sample":"26a_F040644","score":0,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacragaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:105:7312:6495#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":7,"experiment":"wolf_diet","merged_sample":{"26a_F040644":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacgtggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:117:3633:16670#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":24,"experiment":"wolf_diet","merged_sample":{"15a_F730814":13,"29a_F260619":11},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgcaagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:47:15706:3800#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:06)->(T:02)":36,"(A:24)->(C:19)":43},"sample":"29a_F260619","score":407,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaataattcgcaagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:96:17040:12075#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctgaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:110:13684:18231#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":63,"pairing_fast_score":0.783,"pairing_mismatches":{"(A:29)->(G:18)":30,"(C:02)->(G:11)":41},"sample":"26a_F040644","score":600,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcggcagagtactactagcaaca
gcctgaaactcaaagggcttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:53:7759:19356#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":31,"pairing_fast_overlap":62,"pairing_fast_score":0.525,"pairing_mismatches":{"(A:02)->(T:22)":48,"(C:02)->(A:34)":69,"(C:02)->(G:14)":57,"(C:02)->(T:34)":65,"(G:02)->(T:09)":56,"(G:02)->(T:18)":41,"(T:02)->(C:30)":63,"(T:27)->(G:29)":36},"sample":"15a_F730814","score":340,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaatgattctccagagtactaccgtgaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:8:7897:11241#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(C:02)->(G:22)":56,"(C:02)->(T:26)":39,"(C:02)->(T:34)":65,"(G:02)->(C:10)":55,"(T:26)->(G:33)":35},"sample":"15a_F730814","score":981,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtattcgccagagtactacccgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:104:13696:19582#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":24,"pairing_fast_overlap":62,"pairing_fast_score":0.407,"pairing_mismatches":{"(A:32)->(G:22)":32,"(A:33)->(G:09)":52,"(C:02)->(T:30)":65,"(C:21)->(T:20)":39,"(C:30)->(G:09)":57,"(T:22)->(G:14)":35,"(T:34)->(G:19)":25},"sample":"29a_F260619","score":1238,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatccgccagagtactaccggcaat
agcttaaaactcaaggggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:25:19195:14083#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(c:02)->(t:34)":81,"(c:16)->(t:02)":48,"(t:02)->(g:15)":80},"sample":"29a_F260619","score":1152,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagcactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:100:1380:21061#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":63,"pairing_fast_score":0.617,"pairing_mismatches":{"(a:08)->(c:31)":56,"(a:30)->(t:02)":33,"(c:02)->(a:33)":74,"(c:08)->(a:02)":43,"(c:14)->(a:02)":31,"(t:02)->(g:31)":75,"(t:19)->(g:02)":50},"sample":"26a_F040644","score":340,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacactcaataaacacgaatgttcgcccgagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:61:4646:4136#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(c:34)->(a:07)":41},"sample":"29a_F260619","score":2778,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataagctattmcataacaaaattattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:102:11219:15121#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(A:02)->(G:13)":51,"(C:32)->(A:23)":41},"sample":"26a_F040644","score":441,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactgctagcaaca
gcctgaaactcaaaggacttggcggtgctttacatcccc
>HELIUM_000100422_612GNAAXX:7:94:19601:14220#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":63,"pairing_fast_score":0.667,"pairing_mismatches":{"(a:23)->(c:15)":39,"(a:26)->(t:07)":33,"(c:02)->(a:33)":74,"(c:02)->(t:33)":80,"(c:18)->(t:15)":38,"(c:24)->(a:07)":41,"(t:32)->(g:15)":37},"sample":"26a_F040644","score":1324,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtcagccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:14:7192:17634#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":10,"experiment":"wolf_diet","merged_sample":{"26a_F040644":10},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagaactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:60:17061:14253#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":9,"experiment":"wolf_diet","merged_sample":{"26a_F040644":9},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataagcattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:69:19137:1236#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(a:02)->(g:33)":77,"(c:02)->(a:34)":75,"(g:02)->(t:34)":81},"sample":"15a_F730814","score":880,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacacgtaattcatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:103:11969:11036#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactaggaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:62:19012:8950#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(A:15)->(T:15)":58,"(C:34)->(G:02)":29},"sample":"26a_F040644","score":1443,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcawca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:5:1582:18661#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"sample":"29a_F260619","score":28,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctg
>HELIUM_000100422_612GNAAXX:7:117:19288:12695#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcact
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:36:5540:9993#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{},"sample":"26a_F040644","score":2267,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
tcctgaaactcaaaggacttggcggtgctttacatctct
>HELIUM_000100422_612GNAAXX:7:49:19092:15557#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(C:12)->(T:32)":39,"(T:34)->(A:15)":24},"sample":"29a_F260619","score":2023,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacacgtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:100:3844:6799#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3311,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaagggcttggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:73:4723:8766#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttgacggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:25:10972:17033#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":2493,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacatagacattctataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:46:1839:11283#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":8,"experiment":"wolf_diet","merged_sample":{"13a_F730603":8},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacacaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:41:13217:11371#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaacgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:67:10944:19430#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgtcagagtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:90:16847:19958#0/1_sub[28..126] {"ali_dir":"left","count":9,"experiment":"wolf_diet","merged_sample":{"26a_F040644":9},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gtctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:41:9466:18359#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":3919,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttatataaacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:52:18947:13833#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagactactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:99:18812:8601#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(a:02)->(g:27)":77,"(a:31)->(g:02)":31,"(c:02)->(t:34)":81,"(c:21)->(a:02)":33},"sample":"15a_F730814","score":557,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaacattattcgccagggtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:79:7621:9626#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"pairing_mismatches":{},"sample":"26a_F040644","score":2657,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactaccggcaaca
gcccgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:82:16977:18081#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":63,"pairing_fast_score":0.7,"pairing_mismatches":{"(C:02)->(A:21)":68,"(C:27)->(A:02)":22,"(T:02)->(G:09)":50,"(T:02)->(G:15)":35},"sample":"26a_F040644","score":515,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaaggttcgccagagtacgactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:1:16877:14627#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(C:02)->(G:27)":56,"(G:02)->(A:10)":55,"(T:02)->(C:23)":63,"(T:02)->(G:09)":38,"(T:33)->(A:02)":21},"sample":"29a_F260619","score":467,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattagtcgccagagtactaccagcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:48:14483:11354#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":63,"pairing_fast_score":0.7,"pairing_mismatches":{"(a:21)->(c:15)":63,"(a:34)->(g:11)":48,"(a:34)->(g:27)":30,"(c:15)->(t:32)":47,"(c:32)->(t:34)":80},"sample":"26a_F040644","score":2763,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcatgaaactcaaaggacttggcggtgctttacagccct
>HELIUM_000100422_612GNAAXX:7:43:12000:10491#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(A:32)->(G:23)":33,"(C:02)->(G:18)":52},"sample":"26a_F040644","score":1258,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactagtagcaaca
gcctgaaactcaaaggacttggcggtgctttacgtccct
>HELIUM_000100422_612GNAAXX:7:52:2515:3549#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(C:34)->(A:02)":22,"(G:33)->(A:02)":32},"sample":"26a_F040644","score":1960,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaatataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:39:17334:17159#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:02)->(T:08)":49,"(G:02)->(T:22)":55},"sample":"15a_F730814","score":1220,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagttctacctgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:2:14302:17106#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:02)->(C:32)":70,"(C:02)->(A:31)":59,"(T:29)->(G:02)":38,"(T:32)->(A:02)":21},"sample":"29a_F260619","score":723,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaataattcgccagagaactactagcaac
agattaaacctcaaaggacttggcagtgctttataccccg
>HELIUM_000100422_612GNAAXX:7:97:10168:7840#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcagtaaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:16:13563:3432#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(C:02)->(A:33)":68},"sample":"13a_F730603","score":1555,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcaccagagtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:86:13928:12208#0/1_sub[28..127] {"ali_dir":"left","count":6,"experiment":"wolf_diet","merged_sample":{"13a_F730603":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ctagccttaaacacaaatagttatgcaaacaaaactactcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:9:9542:20675#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccat
>HELIUM_000100422_612GNAAXX:7:114:4950:3825#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":12,"experiment":"wolf_diet","merged_sample":{"26a_F040644":12},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgacagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:32:9254:6966#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(c:02)->(a:34)":73,"(t:02)->(c:34)":63},"sample":"26a_F040644","score":888,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttgtcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:81:2103:1960#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagactgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:111:7782:8996#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(c:02)->(g:02)":56,"(t:08)->(c:02)":43},"sample":"13a_F730603","score":23,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgctagagtactaccgscaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:5:8359:14915#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(A:02)->(T:30)":47,"(T:34)->(G:15)":37},"sample":"26a_F040644","score":948,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggactcggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:74:9755:4615#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:02)->(T:06)":52,"(A:34)->(G:06)":37},"sample":"15a_F730814","score":1512,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaggtaattaatataacaaaattattcgccagagtacttccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:16:6986:12689#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(A:02)->(T:20)":49,"(A:34)->(G:02)":37,"(T:33)->(G:02)":21},"sample":"13a_F730603","score":490,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaagtagttatgcaaacaaaactattcgccagagttctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:78:2739:18225#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":63,"pairing_fast_score":0.783,"pairing_mismatches":{"(A:27)->(C:02)":51,"(T:26)->(G:02)":47,"(T:29)->(A:02)":21},"sample":"26a_F040644","score":877,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatcccg
>HELIUM_000100422_612GNAAXX:7:109:12557:9037#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:02)->(C:32)":54},"sample":"13a_F730603","score":1241,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaaataattatataaacaaaattattcgtcagagtactaccggcaac
agcccaaaactcaaaggacttggcggtgcttcacaccctt
>HELIUM_000100422_612GNAAXX:7:29:18512:4449#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":63,"pairing_fast_score":0.75,"pairing_mismatches":{"(A:34)->(G:09)":48,"(C:22)->(G:32)":52,"(T:34)->(G:02)":21},"sample":"26a_F040644","score":1677,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactagcagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:19:15566:19398#0/1_sub[28..127] {"ali_dir":"left","count":25,"experiment":"wolf_diet","merged_sample":{"15a_F730814":14,"29a_F260619":11},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagcactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:93:12538:15367#0/1_sub[28..127] {"ali_dir":"left","count":24,"experiment":"wolf_diet","merged_sample":{"15a_F730814":8,"29a_F260619":16},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0}
ctagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:79:14080:9649#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctgtacatccct
>HELIUM_000100422_612GNAAXX:7:88:17262:5080#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccaaagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:70:13243:7489#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":13,"experiment":"wolf_diet","merged_sample":{"26a_F040644":13},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcagaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:108:12550:5890#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"sample":"29a_F260619","score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtacgagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:22:8044:14897#0/1_sub[78..81] {"ali_dir":"right","count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"sample":"29a_F260619"}
tttc
>HELIUM_000100422_612GNAAXX:7:1:14845:14201#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(A:02)->(T:10)":48,"(G:02)->(A:10)":55},"sample":"26a_F040644","score":1321,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagttctactaacaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:18:6401:21287#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(A:25)->(N:04)":52,"(A:28)->(N:04)":49,"(C:28)->(N:04)":50,"(T:25)->(N:04)":51,"(T:34)->(A:02)":26},"sample":"13a_F730603","score":1123,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaaataattatataaacaaaactattcgccagagtactaccggcaat
agcttaaagctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:56:6962:17278#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:39:12665:7264#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"26a_F040644","score":1759,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatattcgccagagtactactagcaaca
gcctgaaactcaaaggacttagcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:30:2447:15122#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagattactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:24:11458:6844#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(c:34)->(a:09)":42},"sample":"29a_F260619","score":3556,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:21:5643:12411#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":63,"pairing_fast_score":0.883,"pairing_mismatches":{"(c:11)->(a:34)":74},"sample":"26a_F040644","score":3525,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactactagcaaca
gcttaaaactcaaaggacttggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:83:8634:3040#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(G:09)":52,"(A:02)->(T:09)":49,"(C:02)->(T:34)":65},"sample":"13a_F730603","score":1228,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagttctgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctg
>HELIUM_000100422_612GNAAXX:7:2:1734:13615#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(C:02)->(A:34)":67,"(C:02)->(G:07)":41,"(T:34)->(A:02)":20},"sample":"26a_F040644","score":578,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcggcagagtactactagcagca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:120:9585:19730#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":33,"pairing_fast_overlap":62,"pairing_fast_score":0.559,"pairing_mismatches":{"(A:02)->(G:10)":52,"(A:02)->(T:09)":61,"(A:33)->(G:10)":37,"(C:02)->(G:23)":56,"(C:02)->(T:30)":65,"(T:26)->(G:25)":35},"sample":"29a_F260619","score":1309,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat
tgcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:96:13140:9261#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccgtcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:120:3968:20039#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":33,"pairing_fast_overlap":62,"pairing_fast_score":0.559,"pairing_mismatches":{"(A:02)->(G:15)":58,"(C:34)->(G:06)":30,"(G:02)->(T:08)":55,"(T:30)->(C:06)":38,"(T:32)->(G:10)":21},"sample":"15a_F730814","score":1448,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcgat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:19:4075:7081#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3291,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacatagacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:70:13200:18885#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(C:34)->(G:02)":29,"(T:02)->(C:27)":63,"(T:34)->(G:02)":20},"sample":"26a_F040644","score":910,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaataatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:60:13553:20530#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
atgttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:8:18299:11635#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactggcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:99:10175:20053#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":63,"pairing_fast_score":0.65,"pairing_mismatches":{"(C:02)->(A:23)":67,"(T:02)->(G:08)":53,"(T:02)->(G:13)":50,"(T:16)->(G:16)":38},"sample":"26a_F040644","score":685,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtkcgccagagtacgacgagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:46:11662:6103#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(c:07)->(a:34)":58},"sample":"26a_F040644","score":3558,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccccaaacataaacattcaataaacaagaatgttcgccagagtactactagcaatg
gcctaaaactcaaaggacttggtggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:50:4346:4726#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"sample":"15a_F730814","score":2322,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactatcggcaat
agcttagaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:118:6068:19229#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":43,"experiment":"wolf_diet","merged_sample":{"13a_F730603":43},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:63:15519:1559#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(a:05)->(c:02)":40,"(a:14)->(c:02)":43,"(g:29)->(a:22)":76,"(t:14)->(a:02)":64,"(t:28)->(g:02)":38},"sample":"13a_F730603","score":581,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattagcaagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:26:19488:20483#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"13a_F730603":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagggtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:66:12411:4890#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataaaaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:99:3877:7180#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(t:02)->(c:35)":79,"(t:02)->(g:11)":35,"(t:02)->(g:22)":62},"sample":"29a_F260619","score":5,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:60:8309:1539#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactaatagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:83:11562:9219#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtycgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:106:1419:16970#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{"(g:02)->(t:34)":80,"(t:02)->(g:28)":61},"sample":"26a_F040644","score":18,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaatcagtctataacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:32:4845:3166#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(C:02)->(G:28)":56,"(C:34)->(A:02)":30,"(T:02)->(G:25)":39},"sample":"29a_F260619","score":469,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatgcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:53:18471:5398#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagatatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:69:11167:14377#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcamt
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:111:9787:3973#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:02)->(T:09)":58,"(G:30)->(T:08)":41},"sample":"15a_F730814","score":1312,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggctat
agcttaaaactgaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:6:8773:17453#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(C:02)->(G:06)":56},"sample":"29a_F260619","score":1955,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaatgacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:28:7497:1326#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3512,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcttaaaactcaaaggacttggtggtgctttctacccct
>HELIUM_000100422_612GNAAXX:7:22:3462:20622#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:02)->(G:22)":49,"(C:02)->(A:30)":59},"sample":"15a_F730814","score":1814,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat
agcttaaaactcaaaggactcggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:16:3725:11964#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(c:02)->(a:33)":74,"(c:02)->(g:34)":77,"(c:08)->(g:26)":62,"(c:18)->(a:34)":67},"sample":"29a_F260619","score":1254,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggcgctttataccctt
>HELIUM_000100422_612GNAAXX:7:28:12672:7454#0/1_sub[27..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"13a_F730603","score":2911,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaacggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:30:17772:2900#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{"(c:02)->(t:34)":80,"(c:08)->(a:34)":58},"sample":"26a_F040644","score":2952,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacgtaaacattcagtaaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:104:9510:11537#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:02)->(T:08)":49},"sample":"15a_F730814","score":1583,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagttctaccggcaat
agcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:7:1387:12250#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:02)->(T:08)":52,"(A:30)->(G:22)":23,"(C:02)->(G:15)":56,"(C:02)->(T:07)":57},"sample":"15a_F730814","score":102,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtacttccggtaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/1_sub[28..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":64,"pairing_fast_score":0.803,"pairing_mismatches":{"(C:34)->(A:02)":22,"(G:10)->(C:32)":63,"(G:34)->(A:02)":32},"sample":"26a_F040644","score":1426,"score_norm":0.953,"seq_a_single":44,"seq_ab_match":61,"seq_b_single":44}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gccgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:96:6125:7338#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaataaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:99:2865:15695#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcgtaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:4:13834:16217#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(C:02)->(A:34)":59,"(C:02)->(G:35)":56},"sample":"15a_F730814","score":1667,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagtgtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctc
>HELIUM_000100422_612GNAAXX:7:36:1351:10023#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":10,"experiment":"wolf_diet","merged_sample":{"26a_F040644":10},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcacca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:19:15903:14643#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(A:02)->(T:02)":51,"(A:34)->(G:02)":30},"sample":"26a_F040644","score":11,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactwctagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:6:9206:9104#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(a:15)->(t:02)":47,"(a:33)->(g:02)":33,"(c:08)->(a:32)":67},"sample":"26a_F040644","score":1267,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagaactactagcaaca
gcccgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:47:17812:17951#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":3749,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcataggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:51:19442:16409#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"sample":"29a_F260619","score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccgtt
>HELIUM_000100422_612GNAAXX:7:35:13167:18371#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccggagaactactaggaaca
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:102:7624:15512#0/1_sub[27..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":63,"pairing_fast_score":0.683,"pairing_mismatches":{"(A:02)->(G:06)":52,"(A:02)->(G:07)":49,"(C:08)->(A:27)":32,"(G:02)->(A:12)":55,"(T:30)->(G:08)":35},"sample":"29a_F260619","score":913,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctgccagcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:93:15080:3326#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(a:02)->(c:15)":54,"(a:02)->(g:14)":68,"(c:02)->(a:25)":74,"(c:02)->(a:26)":44,"(c:02)->(t:26)":48,"(t:02)->(c:22)":43},"sample":"29a_F260619","score":310,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagcaattaatataacaaaattattcgccagagtactaccggcaat
agcttaagactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:84:8207:3163#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agctcaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:108:13799:19640#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":63,"pairing_fast_score":0.7,"pairing_mismatches":{"(a:34)->(g:02)":32,"(c:02)->(a:33)":73,"(c:18)->(t:02)":38,"(c:28)->(g:15)":41,"(t:33)->(g:02)":34},"sample":"13a_F730603","score":1890,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataatccgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:113:7720:10021#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":33,"pairing_fast_overlap":62,"pairing_fast_score":0.559,"pairing_mismatches":{"(a:02)->(c:28)":43,"(a:02)->(g:34)":77,"(t:02)->(c:28)":54,"(t:02)->(c:29)":50,"(t:02)->(g:11)":80,"(t:22)->(a:12)":35},"sample":"29a_F260619","score":622,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaaatattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:90:14770:18683#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(C:22)->(A:02)":32,"(C:27)->(G:02)":35},"sample":"13a_F730603","score":661,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacacaactatttgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:109:10824:20603#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(C:02)->(A:31)":67,"(C:02)->(T:34)":65,"(T:02)->(G:10)":51,"(T:02)->(G:29)":38},"sample":"15a_F730814","score":812,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattagtcgccagagtacgaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:120:8027:3755#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":11,"experiment":"wolf_diet","merged_sample":{"15a_F730814":10,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaacccaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:42:16525:17476#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":12,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":10},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagaaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:37:18941:9359#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttggccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:61:13302:12850#0/1_sub[28..127] {"ali_dir":"left","count":7,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0}
ttagccctaaacacaagtaattaatataacaaaattaatcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:40:18859:6836#0/1_sub[28..127] {"ali_dir":"left","count":41,"experiment":"wolf_diet","merged_sample":{"15a_F730814":22,"29a_F260619":19},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:42:10581:16688#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"sample":"13a_F730603","score":1865,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccctaaacacaaataattatataaacaaaattattcgccagagtactaccggcaac
agcccaaaactcaaaggacttggcggtgcttcacaccctt
>HELIUM_000100422_612GNAAXX:7:36:4366:16130#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":10,"experiment":"wolf_diet","merged_sample":{"26a_F040644":10},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacatagacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:19:19255:3891#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":63,"pairing_fast_score":0.633,"pairing_mismatches":{"(A:34)->(G:02)":28,"(G:34)->(A:02)":32,"(T:02)->(G:09)":70,"(T:32)->(C:02)":49},"sample":"26a_F040644","score":398,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtattactagcaaca
gcctgaaacgcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:83:10788:9285#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(c:31)->(a:34)":74},"sample":"26a_F040644","score":3870,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
ccctgaaactcaaaggacttggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:64:11263:12699#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(T:34)->(G:02)":38},"sample":"29a_F260619","score":873,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttaggccctt
>HELIUM_000100422_612GNAAXX:7:111:17453:8055#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"13a_F730603":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttgaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:98:8610:13642#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(G:12)":49,"(A:02)->(G:23)":52,"(C:02)->(G:27)":56},"sample":"13a_F730603","score":1530,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:10029:12948#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(C:02)->(A:34)":59,"(C:02)->(G:30)":56,"(G:02)->(T:09)":55},"sample":"29a_F260619","score":1313,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaat
agcttaaaactcaaaggacttggcggtgctttataccctc
>HELIUM_000100422_612GNAAXX:7:50:2313:11850#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttaaccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:14:17361:18819#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":63,"pairing_fast_score":0.733,"pairing_mismatches":{"(A:02)->(T:09)":51,"(A:32)->(G:02)":27,"(T:02)->(G:11)":64},"sample":"26a_F040644","score":719,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtacttctagcaaca
gccggaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:71:11672:8174#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(A:02)->(G:16)":52,"(G:34)->(A:02)":20,"(T:30)->(G:02)":21},"sample":"13a_F730603","score":1196,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgttttataccctt
>HELIUM_000100422_612GNAAXX:7:35:3068:1554#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
taagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:107:8720:14001#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(C:02)->(A:29)":68},"sample":"26a_F040644","score":287,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacgagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:67:15022:1278#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(A:02)->(C:05)":49,"(C:02)->(A:32)":69,"(C:02)->(A:34)":67,"(T:02)->(C:09)":51,"(T:34)->(G:06)":38},"sample":"15a_F730814","score":742,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtcccaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:105:5498:15918#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:02)->(C:33)":54,"(T:02)->(C:08)":35},"sample":"29a_F260619","score":901,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaactattcgccagggtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:50:15408:7710#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(a:08)->(g:33)":77,"(c:02)->(t:29)":81,"(c:23)->(a:16)":52,"(t:30)->(g:02)":36},"sample":"29a_F260619","score":1921,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactcccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:111:18372:20853#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":32,"pairing_fast_overlap":62,"pairing_fast_score":0.542,"pairing_mismatches":{"(A:02)->(C:31)":63,"(A:34)->(G:02)":24,"(C:02)->(G:15)":42,"(C:02)->(G:30)":56,"(C:02)->(G:32)":62,"(T:02)->(G:07)":38,"(T:34)->(G:02)":22},"sample":"29a_F260619","score":483,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattagtcggcagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:67:15014:4973#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3729,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgatttacatccct
>HELIUM_000100422_612GNAAXX:7:32:10486:4941#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:14)->(G:18)":32,"(C:02)->(T:34)":65},"sample":"29a_F260619","score":1451,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacagaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:10:13248:10889#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":63,"pairing_fast_score":0.667,"pairing_mismatches":{"(A:02)->(G:20)":48,"(A:02)->(G:31)":56,"(C:02)->(G:15)":41,"(C:02)->(T:32)":64},"sample":"26a_F040644","score":1135,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcggcagagtgctactaggaaca
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:7:2613:7825#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(g:06)->(c:02)":49},"sample":"26a_F040644","score":1634,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtactagcaaca
gcctgaaactcaaaggacttggcggtgccttacatccct
>HELIUM_000100422_612GNAAXX:7:85:18202:8270#0/1_sub[28..127] {"ali_dir":"left","count":37,"experiment":"wolf_diet","merged_sample":{"15a_F730814":26,"29a_F260619":11},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctc
>HELIUM_000100422_612GNAAXX:7:105:1895:7593#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":11,"experiment":"wolf_diet","merged_sample":{"29a_F260619":11},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccac
agcttaaaactcaaaggacctggcggtgcttcatatccct
>HELIUM_000100422_612GNAAXX:7:5:19312:16368#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(A:02)->(C:30)":54,"(C:02)->(A:26)":42,"(C:02)->(A:32)":59,"(C:02)->(G:10)":56,"(G:02)->(A:09)":47,"(T:31)->(C:02)":22},"sample":"29a_F260619","score":760,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaacagaatactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:115:17539:14092#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(C:02)->(G:09)":42,"(C:02)->(T:34)":65,"(G:02)->(T:23)":55,"(T:02)->(G:09)":35},"sample":"15a_F730814","score":765,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtattcggcagagtactacctgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:18:1855:5923#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttagcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:13:9723:5221#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":25,"pairing_fast_overlap":63,"pairing_fast_score":0.417,"pairing_mismatches":{"(A:02)->(G:18)":66,"(A:10)->(C:02)":42,"(A:30)->(G:02)":33,"(A:30)->(T:02)":51,"(A:34)->(G:02)":30,"(C:28)->(G:02)":52,"(G:02)->(C:26)":63,"(G:16)->(A:02)":44,"(G:29)->(T:02)":32,"(T:34)->(G:02)":20},"sample":"26a_F040644","score":180,"score_norm":0.81,"seq_a_single":45,"seq_ab_match":51,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaaca
gcctggaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:115:14703:8195#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(A:02)->(G:10)":52,"(G:02)->(T:20)":55,"(T:02)->(G:20)":71},"sample":"15a_F730814","score":911,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgcctgcaat
agcttaaaacgcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:118:15661:12736#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatccgcaagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:56:17470:10847#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(t:33)->(c:10)":37},"sample":"26a_F040644","score":2750,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaccaagaatgttcgccagagtactactagcaaca
gcctgaagctcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:50:10384:12141#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{"(T:31)->(G:09)":37},"sample":"26a_F040644","score":1845,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaatcattctataacaaaataattcgccggagaactattagcaaca
gcttaaaactcaaaggacttggtggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:1:10624:6136#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggggctttataccctt
>HELIUM_000100422_612GNAAXX:7:2:11758:7587#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:11975:10484#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(A:02)->(T:06)":52,"(T:02)->(G:14)":51},"sample":"13a_F730603","score":1429,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtacgtccggcaat
agcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:77:1847:15424#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattccccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:9:18549:17048#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(n:02)->(c:34)":72},"sample":"13a_F730603","score":1847,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacccaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:30:9354:14151#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:02)->(G:10)":52,"(C:02)->(G:32)":56,"(G:02)->(A:04)":47,"(T:02)->(G:10)":48},"sample":"15a_F730814","score":1436,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagaagactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:99:2068:1083#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatactctt
>HELIUM_000100422_612GNAAXX:7:55:13242:9085#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagagtgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:38:5612:13272#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":63,"pairing_fast_score":0.733,"pairing_mismatches":{"(a:10)->(g:02)":52,"(a:33)->(g:02)":44,"(c:34)->(a:02)":30},"sample":"15a_F730814","score":710,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggattggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:30:8391:13152#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":17,"experiment":"wolf_diet","merged_sample":{"13a_F730603":17},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaac
agcccaaaactcaaaggacttggcggtgcttcacaccctt
>HELIUM_000100422_612GNAAXX:7:3:5797:14219#0/1_sub[28..127] {"ali_dir":"left","count":22,"experiment":"wolf_diet","merged_sample":{"15a_F730814":16,"29a_F260619":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:48:19275:2712#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcgatgctttataccctt
>HELIUM_000100422_612GNAAXX:7:95:14375:10838#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:37:3004:3752#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttaagcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:90:14966:8399#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaattcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:4:7334:2318#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(c:02)->(t:34)":81,"(t:33)->(a:02)":35},"sample":"15a_F730814","score":2529,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttaaccctaaacacaagtaattaatgtaacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:25:11832:12915#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":63,"pairing_fast_score":0.917,"pairing_mismatches":{"(c:18)->(t:02)":38,"(t:32)->(g:02)":37},"sample":"26a_F040644","score":2007,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtccgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:56:15321:4069#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":9,"experiment":"wolf_diet","merged_sample":{"26a_F040644":9},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacgagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:94:8621:4906#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(T:07)":52,"(G:02)->(C:30)":64,"(T:02)->(G:10)":51,"(T:34)->(A:02)":26,"(T:34)->(G:02)":24},"sample":"13a_F730603","score":443,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaaataattatataaacaaaattattcgccagagtacgtccggcaac
agcccaaaactcaaaggacttggcggtgcttcacaccctt
>HELIUM_000100422_612GNAAXX:7:116:10331:19679#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaagacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:99:8896:7449#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:18)->(G:18)":34,"(T:02)->(G:10)":51},"sample":"13a_F730603","score":1292,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaarctattcgccagagtacgaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:115:10554:9352#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:02)->(C:34)":57,"(A:02)->(G:18)":36,"(A:02)->(T:24)":39,"(C:02)->(G:09)":42,"(C:02)->(G:30)":56,"(C:07)->(A:30)":20,"(G:02)->(C:23)":55,"(T:02)->(G:21)":38},"sample":"15a_F730814","score":512,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaatgagtcggcagagtactacccgcaat
agcttaaaactcaaaggactgggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:3:2784:5259#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":63,"pairing_fast_score":0.717,"pairing_mismatches":{"(C:02)->(G:09)":41,"(G:02)->(A:16)":55,"(T:34)->(A:02)":20},"sample":"26a_F040644","score":266,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcggcagagtactactaacaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:117:8364:18921#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(a:34)->(g:02)":31,"(c:05)->(t:02)":39,"(c:06)->(t:02)":48,"(g:05)->(c:02)":43},"sample":"15a_F730814","score":74,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatccgcgagagcactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:17:13574:5763#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(a:07)->(t:02)":39,"(t:31)->(c:02)":38},"sample":"13a_F730603","score":7,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactatacgccagaggactactagcaat
agcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:53:6191:8164#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"29a_F260619","score":3452,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaagtaattacacaaacaaaattgttcaccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:2:3786:20474#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":33,"pairing_fast_overlap":63,"pairing_fast_score":0.55,"pairing_mismatches":{"(a:30)->(g:02)":33,"(c:21)->(g:02)":44,"(c:22)->(g:02)":41,"(c:25)->(a:02)":31,"(g:02)->(t:16)":79,"(t:02)->(c:30)":78,"(t:08)->(c:02)":52,"(t:14)->(g:02)":37},"sample":"26a_F040644","score":816,"score_norm":0.857,"seq_a_single":45,"seq_ab_match":54,"seq_b_single":45}
ttagccctaaacataaacattcaataaacacgaatgttcgccacagtactattagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:111:6134:7059#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":63,"pairing_fast_score":0.6,"pairing_mismatches":{"(A:02)->(G:24)":49,"(C:02)->(A:33)":59,"(T:02)->(G:18)":64,"(T:25)->(G:28)":38,"(T:30)->(G:14)":36},"sample":"13a_F730603","score":1335,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ctagccttaaacacaaatagttatgcaaacaaaactagtcgccagagtgctaccggcaat
agcgtaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:30:1957:19526#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(A:27)->(G:10)":52,"(A:31)->(G:15)":31,"(C:25)->(G:07)":50},"sample":"13a_F730603","score":2637,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatggttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:15:16388:8415#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"26a_F040644","score":1329,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttgggggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:119:6544:19425#0/1_sub[28..127] {"ali_dir":"left","count":16,"experiment":"wolf_diet","merged_sample":{"15a_F730814":13,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacccgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:44:10589:1749#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(c:06)->(a:02)":46,"(c:10)->(a:02)":44,"(c:27)->(a:02)":32},"sample":"29a_F260619","score":1410,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacacaattattcgcccgcgtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:117:9856:4050#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":16,"experiment":"wolf_diet","merged_sample":{"26a_F040644":16},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgccttacatccct
>HELIUM_000100422_612GNAAXX:7:76:11088:13251#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgcgagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:44:19298:19344#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(G:33)->(A:24)":25},"sample":"13a_F730603","score":2936,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaatacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:82:13334:18499#0/1_sub[28..127] {"ali_dir":"left","count":6,"experiment":"wolf_diet","merged_sample":{"29a_F260619":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"sample":"29a_F260619"}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:11:10094:15139#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":31,"pairing_fast_overlap":62,"pairing_fast_score":0.525,"pairing_mismatches":{"(A:02)->(C:30)":72,"(A:02)->(G:07)":52,"(A:02)->(T:22)":36,"(C:02)->(A:33)":59,"(C:02)->(G:07)":56},"sample":"29a_F260619","score":621,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcggcagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:69:4826:14391#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(t:34)->(c:09)":38},"sample":"13a_F730603","score":3359,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactgttcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:96:10854:13045#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:18)->(G:18)":52},"sample":"15a_F730814","score":2235,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactrccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:68:6087:8107#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(G:20)":49,"(T:02)->(G:14)":51,"(T:27)->(G:21)":38,"(T:34)->(A:02)":21},"sample":"15a_F730814","score":812,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgcgaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:111:16361:2173#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(C:07)->(T:02)":39},"sample":"29a_F260619","score":371,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
tcagccctaaacacaagtaattaatataacaaaattatccgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:5:6876:17353#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:02)->(G:11)":58,"(G:02)->(A:08)":45},"sample":"15a_F730814","score":1354,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccaaagtactaccggcgat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:72:3083:18443#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"26a_F040644","score":1920,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggagttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:108:11272:1128#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactactagcaat
agcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:24:6794:13510#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":63,"pairing_fast_score":0.717,"pairing_mismatches":{"(A:02)->(G:09)":48,"(C:02)->(A:33)":58},"sample":"26a_F040644","score":921,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgctactagcaaca
gcctgaaactcaaaggacgtggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:96:6735:11074#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(c:32)->(a:30)":73},"sample":"26a_F040644","score":3701,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagagtactactagcaaca
gcctgaaactcacaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:75:11341:17945#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3396,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagctaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:88:15278:20903#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":33,"pairing_fast_overlap":62,"pairing_fast_score":0.559,"pairing_mismatches":{"(a:02)->(c:32)":57,"(a:02)->(c:34)":70,"(c:02)->(a:30)":58,"(c:02)->(a:32)":69,"(c:02)->(a:34)":67,"(c:02)->(g:34)":77,"(c:23)->(t:23)":51,"(c:29)->(a:02)":42},"sample":"13a_F730603","score":391,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtacyaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:119:8582:8637#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3061,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactaacaaca
gcctgaaactcaaaggacttggtggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:106:12615:12064#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"pairing_mismatches":{},"sample":"26a_F040644","score":1259,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacg
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:72:1417:17793#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:02)->(G:11)":58,"(C:34)->(A:13)":30,"(T:22)->(G:28)":35},"sample":"15a_F730814","score":1440,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtattcgccagagtactaccggcgat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:112:2223:5401#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattgttcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(A:02)->(T:06)":49,"(C:02)->(G:32)":56},"sample":"29a_F260619","score":1658,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagttctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctg
>HELIUM_000100422_612GNAAXX:7:35:18011:8521#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":16,"experiment":"wolf_diet","merged_sample":{"15a_F730814":8,"29a_F260619":8},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
tcagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:100:1906:2415#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(a:08)->(c:02)":40,"(a:18)->(c:34)":70,"(c:06)->(a:22)":59,"(c:07)->(a:35)":69,"(c:22)->(a:34)":75,"(t:09)->(g:22)":60},"sample":"29a_F260619","score":1125,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattagccagagtactaccggcaag
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:120:10826:9352#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(a:02)->(g:34)":77,"(c:10)->(a:02)":59,"(c:25)->(a:34)":69,"(c:26)->(a:34)":67},"sample":"13a_F730603","score":687,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcact
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:117:7167:17979#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":63,"pairing_fast_score":0.617,"pairing_mismatches":{"(a:08)->(t:02)":47,"(a:33)->(t:02)":33,"(c:02)->(t:34)":80,"(c:14)->(a:02)":58,"(c:15)->(g:02)":62},"sample":"26a_F040644","score":190,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagaactactagcacca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:8:3288:3960#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(A:02)->(C:34)":69,"(T:32)->(C:02)":21},"sample":"29a_F260619","score":2157,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataagctattccatagcaaaataattcgccagagaactactagcaaca
gattaaacctcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:105:9895:19591#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(c:02)->(a:30)":69,"(c:02)->(a:34)":67,"(c:07)->(a:02)":29,"(t:07)->(c:02)":30},"sample":"15a_F730814","score":1917,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatatactaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:40:18436:16490#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"pairing_mismatches":{"(c:02)->(a:31)":69,"(c:02)->(t:25)":81},"sample":"13a_F730603","score":581,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggagctttataccctt
>HELIUM_000100422_612GNAAXX:7:119:3056:8430#0/1_sub[27..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(c:02)->(g:02)":56},"sample":"13a_F730603","score":17,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccgscaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:119:13502:6630#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(c:02)->(t:34)":65,"(c:12)->(a:02)":33,"(c:17)->(a:02)":32,"(g:02)->(c:32)":70},"sample":"13a_F730603","score":217,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaccactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:115:14185:16488#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(G:02)->(C:10)":55},"sample":"13a_F730603","score":1518,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacccgcaat
agcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:4:19326:20521#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(a:07)->(c:02)":42,"(a:08)->(t:02)":47,"(t:15)->(g:02)":41},"sample":"26a_F040644","score":656,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgtaagagaactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:50:8875:20172#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(a:02)->(c:34)":72,"(c:02)->(a:34)":69,"(c:02)->(t:33)":71,"(t:02)->(c:07)":64},"sample":"29a_F260619","score":1670,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaataaatataacaaaattattcgccagagtactaccggcaat
agcctaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:74:16625:17507#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:16)->(C:26)":54,"(G:19)->(A:24)":41,"(T:02)->(G:14)":64,"(T:26)->(G:08)":35,"(T:34)->(A:02)":21},"sample":"29a_F260619","score":1394,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcaccagagtactaccggcaat
agcgtaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:64:4232:20295#0/1_sub[28..126] {"ali_dir":"left","count":18,"experiment":"wolf_diet","merged_sample":{"26a_F040644":18},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataaacattcaataaacaaggatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:7:6016:14767#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtacgtctagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:31:10627:17627#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":8,"experiment":"wolf_diet","merged_sample":{"13a_F730603":8},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcagt
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:103:15658:7277#0/1_sub[28..127] {"ali_dir":"left","count":9,"experiment":"wolf_diet","merged_sample":{"13a_F730603":9},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagcactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:19:1396:14092#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(a:31)->(g:02)":48,"(a:34)->(g:02)":30,"(t:31)->(g:02)":63},"sample":"26a_F040644","score":531,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacatagatacttttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:92:6070:1284#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(A:24)->(G:02)":26,"(C:18)->(A:02)":24,"(T:02)->(G:27)":60},"sample":"15a_F730814","score":285,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattactataacaaaattattcgccagagtactaccggcaag
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:35:7426:10274#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgagactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:85:2211:20855#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{},"sample":"26a_F040644","score":1794,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaatcattctataacaaaataattcgccagagtactactagcaaca
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:40:4112:15755#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(g:16)->(c:02)":29},"sample":"26a_F040644","score":3412,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaagaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:70:10801:10253#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(A:02)->(G:06)":51,"(C:02)->(A:26)":58},"sample":"26a_F040644","score":633,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaatcattctataacaaaataattcgccggagaactgctagcaaca
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:11:12089:1704#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"13a_F730603":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactatcggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:61:19754:12716#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(a:02)->(g:07)":80,"(c:02)->(a:24)":75,"(c:02)->(g:14)":77,"(c:02)->(t:22)":81,"(g:02)->(t:15)":76,"(g:29)->(a:13)":47,"(t:02)->(a:33)":67,"(t:26)->(g:19)":39},"sample":"13a_F730603","score":793,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaatgacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:112:2889:7743#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(c:02)->(a:34)":74,"(c:02)->(g:34)":77},"sample":"29a_F260619","score":651,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacacgtacttattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:55:15914:11738#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(a:02)->(g:34)":76,"(a:10)->(c:32)":42},"sample":"26a_F040644","score":3166,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaatattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggagctttacatccct
>HELIUM_000100422_612GNAAXX:7:18:2484:20392#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":62,"pairing_fast_score":0.593,"pairing_mismatches":{"(A:02)->(G:06)":52,"(A:02)->(T:34)":65,"(C:02)->(A:34)":68,"(T:02)->(G:28)":39,"(T:26)->(A:02)":21},"sample":"15a_F730814","score":30,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatgcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:102:15142:4355#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(a:07)->(t:15)":47,"(c:12)->(g:34)":61,"(t:07)->(c:10)":49,"(t:10)->(c:10)":37},"sample":"26a_F040644","score":2738,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgytcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:68:9010:11268#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":63,"pairing_fast_score":0.633,"pairing_mismatches":{"(a:10)->(c:33)":71,"(a:27)->(c:02)":29,"(c:09)->(a:34)":72,"(c:10)->(t:34)":70,"(t:07)->(a:02)":34,"(t:08)->(c:02)":39},"sample":"15a_F730814","score":1642,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacacaagtaattaatataaaaaattatttgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:24:7635:13158#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(c:02)->(a:19)":69,"(c:02)->(a:34)":67,"(c:02)->(t:34)":81,"(c:08)->(t:02)":39,"(c:10)->(t:02)":48,"(t:02)->(g:32)":77,"(t:23)->(g:02)":35},"sample":"15a_F730814","score":564,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatccgccagagcactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:52:16971:11050#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(a:02)->(c:31)":70},"sample":"29a_F260619","score":1128,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaactaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:65:2530:14539#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"sample":"15a_F730814","score":2981,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattamtataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:94:5026:13290#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:33)->(G:02)":52,"(T:34)->(G:02)":25},"sample":"29a_F260619","score":85,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agctcaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:115:9326:3427#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(C:02)->(A:34)":67},"sample":"15a_F730814","score":1917,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacagaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgccttataccctt
>HELIUM_000100422_612GNAAXX:7:18:8799:9720#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(T:32)->(G:02)":21},"sample":"29a_F260619","score":2130,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:74:10246:19861#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":6,"experiment":"wolf_diet","merged_sample":{"26a_F040644":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggactgggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:10:17907:11073#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctc
>HELIUM_000100422_612GNAAXX:7:115:13056:20438#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(a:34)->(g:02)":31,"(c:02)->(a:34)":61,"(c:02)->(g:34)":77,"(t:14)->(g:29)":38},"sample":"13a_F730603","score":703,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctggccttaaacacaaatagttatgaaaacaaaactagtcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:76:11643:4174#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:19:16277:9261#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3956,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcgaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:19:9359:17429#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcgat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:21:1400:5072#0/1_sub[28..127] {"ali_dir":"left","count":11,"experiment":"wolf_diet","merged_sample":{"13a_F730603":11},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:55:7029:21183#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":9,"experiment":"wolf_diet","merged_sample":{"15a_F730814":8,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggtggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:9:12429:12522#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":32,"pairing_fast_overlap":62,"pairing_fast_score":0.542,"pairing_mismatches":{"(A:02)->(C:21)":54,"(A:02)->(G:11)":49,"(A:27)->(N:02)":29,"(C:02)->(A:25)":69,"(C:02)->(G:23)":56,"(C:02)->(T:32)":65,"(C:15)->(T:02)":39},"sample":"29a_F260619","score":220,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatccgccagagtgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:22:4600:12039#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":8,"experiment":"wolf_diet","merged_sample":{"13a_F730603":8},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactactggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:99:3867:10605#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:32:3235:10513#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{},"sample":"13a_F730603","score":3615,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactactcaccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:102:17087:21253#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(A:02)->(C:17)":54,"(A:34)->(G:24)":32,"(C:02)->(T:32)":65,"(T:29)->(G:26)":35},"sample":"29a_F260619","score":926,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattccccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:103:4748:15078#0/1_sub[28..127] {"ali_dir":"left","count":6,"experiment":"wolf_diet","merged_sample":{"13a_F730603":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ctagccttaaacgcaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:41:18449:21122#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"13a_F730603","seq_a_single":45,"seq_b_single":45}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccct
>HELIUM_000100422_612GNAAXX:7:118:5490:20581#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"15a_F730814":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtcctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:108:1985:14831#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(A:28)->(G:02)":27,"(A:34)->(G:02)":29,"(C:28)->(G:06)":42,"(G:02)->(A:07)":56,"(G:34)->(A:02)":20},"sample":"13a_F730603","score":252,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccgacaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:30:5726:7656#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":8,"experiment":"wolf_diet","merged_sample":{"15a_F730814":5,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtacttaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:109:9480:13014#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":2851,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggccgtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:108:10947:13120#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":62,"pairing_fast_score":0.983,"pairing_mismatches":{"(g:02)->(t:34)":81},"sample":"13a_F730603","score":2496,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacgaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:28:6659:19816#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(A:02)->(T:10)":49,"(C:02)->(G:33)":56,"(T:33)->(G:10)":35},"sample":"29a_F260619","score":1600,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagttctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:99:7632:4115#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaacactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:48:15074:1707#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":63,"pairing_fast_score":0.567,"pairing_mismatches":{"(a:02)->(c:34)":71,"(a:10)->(c:02)":56,"(c:02)->(g:31)":76,"(c:02)->(t:12)":62,"(c:24)->(a:02)":41,"(g:10)->(t:02)":38,"(t:24)->(c:02)":37},"sample":"26a_F040644","score":0,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtgcgccagagtactactagaaaca
gtctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:94:12935:19738#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(a:28)->(c:34)":65},"sample":"13a_F730603","score":3295,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaacagttatgcaaacaaaattattcgccagagtactaccggcaac
agcccaaaactcaaaggacttggcggtgcttcacaccctt
>HELIUM_000100422_612GNAAXX:7:92:15623:12608#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{},"sample":"26a_F040644","score":761,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaatcattctataacaaaataattcgccggagaactactagcaaca
gcttaaaactcaaaggacttggcggtgcctcacgtccct
>HELIUM_000100422_612GNAAXX:7:99:9351:13090#0/1_sub[28..127] {"ali_dir":"left","count":260,"experiment":"wolf_diet","merged_sample":{"29a_F260619":260},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"sample":"29a_F260619"}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:27:1622:8578#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":63,"pairing_fast_score":0.683,"pairing_mismatches":{"(A:07)->(G:21)":54,"(A:28)->(G:29)":43,"(C:10)->(A:26)":58,"(G:31)->(A:22)":44},"sample":"26a_F040644","score":2039,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccggagtactactggcaaca
gcctgaaactcaaaggacgtggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:37:19168:8597#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(c:02)->(a:34)":67,"(c:28)->(a:28)":46},"sample":"29a_F260619","score":2237,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagmgtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:37:5037:5790#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"13a_F730603":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactagcggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:8:11931:2229#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:02)->(G:10)":49},"sample":"29a_F260619","score":1402,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatatagcaaaattattcgccagagtgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:9:8337:12329#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacagtcaataaacaaggatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:100:16289:13186#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(a:10)->(c:31)":42,"(t:02)->(g:15)":79},"sample":"26a_F040644","score":1170,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactggcaata
gcttaaaactcaaaggacgtggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:72:12349:20235#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{},"sample":"26a_F040644","score":3140,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctatatatccct
>HELIUM_000100422_612GNAAXX:7:97:14056:17436#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":30,"pairing_fast_overlap":62,"pairing_fast_score":0.508,"pairing_mismatches":{"(A:02)->(G:16)":49,"(A:34)->(G:02)":37,"(C:02)->(T:32)":65,"(G:02)->(A:07)":56,"(G:02)->(T:06)":62,"(T:32)->(G:02)":21,"(T:34)->(G:02)":25},"sample":"15a_F730814","score":268,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccgacaat
atcttaaaactcaaatgacctggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:114:16385:12527#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggctat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:15:12045:12444#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(A:02)->(T:30)":71,"(C:02)->(A:34)":67,"(C:02)->(G:22)":62,"(T:23)->(G:31)":56},"sample":"29a_F260619","score":1876,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
tcagccctaaacacacgtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:17:15759:16994#0/1_sub[28..126] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":64,"pairing_fast_score":0.787,"pairing_mismatches":{"(A:02)->(G:15)":68},"sample":"26a_F040644","score":1365,"score_norm":0.938,"seq_a_single":44,"seq_ab_match":60,"seq_b_single":44}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaagctcaaaggacgtggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:117:2305:16083#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgcttcataccctt
>HELIUM_000100422_612GNAAXX:7:78:12427:4406#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:3:2540:6220#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataataaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:1:8672:21076#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(c:08)->(a:32)":73},"sample":"26a_F040644","score":1652,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcagtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:59:6581:2704#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(A:02)->(C:34)":54,"(A:02)->(T:32)":51,"(C:02)->(G:33)":56},"sample":"15a_F730814","score":1098,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtattaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:18:1967:2789#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:02)->(G:08)":49},"sample":"15a_F730814","score":1360,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat
agcttaaaactcaaaggacttggtggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:98:12969:15041#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(A:02)->(C:29)":54,"(A:22)->(G:31)":34,"(A:34)->(G:02)":28},"sample":"13a_F730603","score":536,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaagctattcgccagagtactaccggcaat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:51:1436:21165#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":63,"pairing_fast_score":0.683,"pairing_mismatches":{"(C:02)->(A:15)":27,"(C:02)->(A:16)":31,"(C:02)->(A:24)":24,"(C:02)->(T:16)":38,"(C:06)->(A:02)":21},"sample":"26a_F040644","score":97,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaccatccaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:45:9633:19662#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(a:02)->(c:32)":72,"(c:02)->(a:31)":73,"(t:02)->(g:18)":71,"(t:24)->(g:24)":35},"sample":"29a_F260619","score":1691,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattacacaaacaaaaktgttcaccagagtactagcggcaac
agcttaaaacgcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:116:9795:5038#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(a:02)->(g:34)":77,"(a:27)->(t:33)":66},"sample":"29a_F260619","score":2740,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agctttaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:114:14572:16729#0/1_sub[27..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":64,"pairing_fast_score":0.852,"pairing_mismatches":{"(A:02)->(G:11)":51},"sample":"26a_F040644","score":1157,"score_norm":0.953,"seq_a_single":44,"seq_ab_match":61,"seq_b_single":44}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactgctagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:116:2017:5785#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":63,"pairing_fast_score":0.667,"pairing_mismatches":{"(A:33)->(G:02)":27,"(G:02)->(A:33)":67,"(T:02)->(C:34)":63,"(T:15)->(G:13)":38},"sample":"26a_F040644","score":1010,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggctgtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:76:4494:16489#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:30)->(C:19)":52,"(C:11)->(A:31)":59,"(C:34)->(A:34)":67},"sample":"29a_F260619","score":2852,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttamaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:118:12921:19594#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(A:02)->(G:07)":58,"(A:34)->(G:22)":33,"(C:02)->(G:17)":50,"(T:34)->(A:02)":21},"sample":"29a_F260619","score":601,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtagtaccggcgat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:70:4158:18787#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":33,"pairing_fast_overlap":62,"pairing_fast_score":0.559,"pairing_mismatches":{"(a:02)->(c:29)":72,"(c:02)->(g:32)":77,"(c:02)->(t:33)":81,"(c:10)->(t:02)":48,"(c:22)->(a:02)":42,"(c:27)->(a:02)":30,"(t:02)->(g:10)":80,"(t:29)->(c:02)":51},"sample":"29a_F260619","score":594,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttagccctaaacacaagaaattaatataacaaaattattcgccagagcactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttatacccgt
>HELIUM_000100422_612GNAAXX:7:60:8067:20227#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:09)->(T:29)":36,"(C:15)->(G:33)":56},"sample":"29a_F260619","score":2364,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaataaaacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:42:4308:2464#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":33,"pairing_fast_overlap":62,"pairing_fast_score":0.559,"pairing_mismatches":{"(a:30)->(c:08)":58,"(a:34)->(g:02)":31,"(t:11)->(a:02)":44,"(t:32)->(g:02)":38},"sample":"15a_F730814","score":1169,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgcctgagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:3:5675:6889#0/1_sub[28..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":61,"pairing_fast_overlap":64,"pairing_fast_score":1,"sample":"26a_F040644","score":1738,"score_norm":1,"seq_a_single":44,"seq_ab_match":64,"seq_b_single":44}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:95:5652:11000#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(c:15)->(a:19)":42,"(g:02)->(t:34)":81},"sample":"29a_F260619","score":3423,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaataattcgacagagaactactagcaac
agattaaacctcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:15:12854:18952#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":10,"experiment":"wolf_diet","merged_sample":{"29a_F260619":10},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"29a_F260619","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaattattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:107:10538:14748#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":62,"pairing_fast_score":0.983,"pairing_mismatches":{},"sample":"13a_F730603","score":1017,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaattattcgccagagtactaccggcaac
agcccaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:47:14501:20981#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaatgacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:119:10342:7620#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(A:02)->(G:09)":52,"(A:02)->(G:12)":37,"(A:02)->(T:34)":65,"(C:02)->(A:30)":59,"(C:02)->(G:33)":56},"sample":"15a_F730814","score":729,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
tcagccctaaacacaagtaattaatataacaaaattgttcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:2:18300:6345#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(a:02)->(c:31)":72,"(a:02)->(g:29)":77,"(c:02)->(t:18)":48,"(c:02)->(t:34)":65,"(t:02)->(c:28)":79,"(t:08)->(g:02)":39},"sample":"29a_F260619","score":117,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatagcctt
>HELIUM_000100422_612GNAAXX:7:9:3051:13944#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":62,"pairing_fast_score":0.593,"pairing_mismatches":{"(A:02)->(C:13)":54,"(A:02)->(C:25)":43,"(A:02)->(T:29)":65,"(A:34)->(N:02)":29,"(G:02)->(C:10)":55},"sample":"13a_F730603","score":255,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagactactacccgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:37:18425:17908#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":2340,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacatgaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatacct
>HELIUM_000100422_612GNAAXX:7:117:13211:1613#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":63,"pairing_fast_score":0.617,"pairing_mismatches":{"(A:22)->(G:02)":57,"(A:29)->(T:02)":48,"(A:34)->(C:02)":30,"(C:13)->(A:02)":58,"(C:26)->(G:02)":49,"(T:32)->(G:02)":38,"(T:34)->(G:02)":20},"sample":"26a_F040644","score":543,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcacca
gcctgaaactcaaaggacctggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:106:13594:15967#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(C:02)->(G:32)":56,"(T:02)->(C:15)":35,"(T:02)->(G:17)":71},"sample":"15a_F730814","score":717,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaactattcgccagagtactaccggcaat
agcttaaaacgcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:110:4537:10123#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(A:34)->(T:02)":32,"(C:24)->(G:32)":56,"(C:34)->(A:02)":26,"(T:32)->(G:02)":21},"sample":"29a_F260619","score":724,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
tcagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:115:1658:12655#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(C:14)->(T:34)":65,"(C:29)->(A:02)":20},"sample":"29a_F260619","score":2818,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagcacttaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:108:12033:7965#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"pairing_mismatches":{},"sample":"26a_F040644","score":3379,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgtcagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacacccct
>HELIUM_000100422_612GNAAXX:7:14:12232:3448#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(T:33)->(G:07)":35},"sample":"15a_F730814","score":1700,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtagttaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctc
>HELIUM_000100422_612GNAAXX:7:12:10601:19345#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(c:21)->(a:34)":59},"sample":"13a_F730603","score":1455,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaataattcgccagaggactactagcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:15:12768:19287#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(G:02)->(T:09)":47,"(T:02)->(G:08)":38,"(T:34)->(G:15)":22},"sample":"26a_F040644","score":1097,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataatgcgccagagtactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:29:6638:16804#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(a:17)->(t:02)":36,"(c:21)->(g:02)":42,"(c:33)->(t:02)":53,"(t:26)->(g:02)":35},"sample":"29a_F260619","score":419,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccccaaacacaagtaattaatataacaaaataattcgccagagtactaccggcaat
agcttaaaactcaaaggacctggcggtgctctataccctt
>HELIUM_000100422_612GNAAXX:7:11:9258:14485#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":18,"experiment":"wolf_diet","merged_sample":{"15a_F730814":9,"29a_F260619":9},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccgccaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:17:6470:21039#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(a:10)->(c:28)":52,"(t:28)->(g:07)":37},"sample":"13a_F730603","score":3020,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ctagcttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:11:15719:20052#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":10,"experiment":"wolf_diet","merged_sample":{"15a_F730814":5,"29a_F260619":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagaggactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:41:9660:12131#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(T:18)":48,"(C:02)->(G:34)":56,"(C:02)->(T:34)":65},"sample":"29a_F260619","score":1472,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggactcggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:83:19093:8407#0/1_sub[28..126] {"ali_dir":"left","count":18,"experiment":"wolf_diet","merged_sample":{"26a_F040644":18},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaacgcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:25:9451:18923#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"13a_F730603":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaagcaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:106:18215:20026#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacagaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:33:13834:11523#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(t:33)->(a:07)":35},"sample":"29a_F260619","score":3273,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:28:13733:6118#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"13a_F730603":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacgaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:55:5666:16200#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcttgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:66:4591:21141#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagcactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:100:4614:15721#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(A:08)->(T:02)":36,"(C:02)->(A:34)":66,"(C:09)->(G:11)":56,"(C:28)->(G:07)":57,"(T:02)->(G:08)":71,"(T:34)->(A:02)":25},"sample":"29a_F260619","score":781,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaataattcgccagagtactaccggcaat
agcttaaaacgcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:7:1913:8838#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(A:02)->(C:25)":54,"(A:02)->(T:08)":52,"(C:02)->(A:31)":59,"(T:02)->(G:09)":36},"sample":"15a_F730814","score":798,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaatgattcgccagagtacttccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:1:4513:20277#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaaccattctataacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:35:4537:1178#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(c:02)->(t:34)":81,"(c:23)->(a:23)":61},"sample":"29a_F260619","score":2730,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
mgcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:77:11129:15983#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(c:11)->(a:02)":45},"sample":"26a_F040644","score":1661,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagcggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatagccct
>HELIUM_000100422_612GNAAXX:7:7:3441:13679#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":63,"pairing_fast_score":0.75,"pairing_mismatches":{"(A:02)->(T:07)":51,"(C:02)->(G:06)":56,"(T:02)->(G:24)":50},"sample":"26a_F040644","score":1668,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtacgtctaggaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:119:14269:18878#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(a:07)->(c:02)":63,"(c:07)->(a:02)":59,"(c:10)->(g:34)":77,"(c:12)->(g:02)":56,"(c:22)->(t:34)":81,"(t:33)->(a:02)":35},"sample":"15a_F730814","score":860,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccgccact
agattaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:119:11055:2347#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":63,"pairing_fast_score":0.75,"pairing_mismatches":{"(A:34)->(T:02)":19,"(C:02)->(A:14)":41},"sample":"26a_F040644","score":1029,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgacagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:14:17760:17476#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcgatgctttataccctt
>HELIUM_000100422_612GNAAXX:7:102:18681:13424#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(c:23)->(a:23)":68},"sample":"26a_F040644","score":3311,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaamctcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:110:13756:17419#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(a:10)->(c:25)":43,"(c:02)->(t:34)":81,"(c:14)->(g:26)":42,"(c:14)->(t:02)":39,"(c:26)->(a:28)":59,"(c:32)->(g:23)":56,"(c:34)->(g:18)":53},"sample":"29a_F260619","score":1029,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatccggcagagtactaccgccaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:110:2972:3403#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3155,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctagacataaacattcaataaacaagaatgttcgtcagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:39:8990:3897#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":4028,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatatcctt
>HELIUM_000100422_612GNAAXX:7:114:17523:6141#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(a:09)->(g:15)":35},"sample":"13a_F730603","score":3631,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaatgattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:50:18708:3148#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"pairing_mismatches":{"(a:02)->(t:22)":65,"(c:02)->(a:34)":68},"sample":"13a_F730603","score":2463,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatactctt
>HELIUM_000100422_612GNAAXX:7:29:5739:1887#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(a:02)->(c:34)":80,"(c:02)->(a:31)":67,"(c:02)->(g:34)":77,"(c:15)->(a:02)":46,"(t:34)->(g:02)":36},"sample":"29a_F260619","score":1030,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagcacactacgagccac
agcttaaaactcaaaggacctggcggtgcttcatatccct
>HELIUM_000100422_612GNAAXX:7:39:3032:19855#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(T:02)->(G:10)":64,"(T:28)->(G:02)":51},"sample":"15a_F730814","score":233,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcgtaaaactcaaaggacttggcggtgctttatacccgt
>HELIUM_000100422_612GNAAXX:7:15:15506:17587#0/1_sub[28..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":64,"pairing_fast_score":0.852,"pairing_mismatches":{"(C:22)->(A:27)":57,"(C:34)->(A:02)":18},"sample":"26a_F040644","score":2873,"score_norm":0.953,"seq_a_single":44,"seq_ab_match":61,"seq_b_single":44}
ttagccctaaacataaacattaataaacaagaatgttcgccagagtactactagcaacag
cctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:22:2466:16619#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(a:06)->(c:34)":63,"(c:02)->(t:31)":81},"sample":"29a_F260619","score":3489,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaatttattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:8:19462:9995#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatkcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:72:17010:13290#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgcgttataccctt
>HELIUM_000100422_612GNAAXX:7:31:9276:14165#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":6,"experiment":"wolf_diet","merged_sample":{"26a_F040644":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactatcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:30:4120:8880#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":14,"experiment":"wolf_diet","merged_sample":{"15a_F730814":11,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatatagcaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:5:19261:19723#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcgaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:57:18237:6765#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatgcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:91:7608:19157#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":62,"pairing_fast_score":0.593,"pairing_mismatches":{"(a:02)->(c:34)":72,"(a:08)->(t:15)":48,"(c:02)->(a:32)":73,"(c:02)->(a:34)":75,"(c:02)->(g:34)":76,"(c:11)->(a:13)":49,"(c:20)->(a:30)":61,"(c:21)->(g:32)":45,"(t:16)->(g:24)":38},"sample":"29a_F260619","score":1356,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattggtcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:70:5074:12547#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:02)->(G:07)":49,"(C:02)->(A:13)":59,"(T:34)->(A:07)":27},"sample":"29a_F260619","score":1235,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtgctaccggcaat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:58:2809:10138#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:02)->(C:10)":52,"(A:02)->(T:07)":58},"sample":"13a_F730603","score":434,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactcccggctat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:116:18987:2074#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:02)->(C:31)":54,"(A:02)->(G:28)":58,"(C:02)->(G:34)":56,"(T:02)->(G:18)":48,"(T:29)->(A:18)":35,"(T:30)->(G:33)":21},"sample":"15a_F730814","score":1250,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaagtaatataacaaaattattcgccagaggactaccggcgat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:100:18302:8172#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(c:10)->(a:02)":33,"(t:02)->(g:07)":80},"sample":"13a_F730603","score":1860,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaacactattcgccagagtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:37:14964:19175#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"15a_F730814":5,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaactaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:6:18373:20472#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":63,"pairing_fast_score":0.667,"pairing_mismatches":{"(c:09)->(a:32)":68,"(c:19)->(t:02)":38,"(c:23)->(a:17)":73,"(c:33)->(a:02)":29},"sample":"26a_F040644","score":1064,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtccgccagagtactactagcaaca
gcctgaaactcacaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:37:13773:8945#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(a:09)->(c:34)":71},"sample":"26a_F040644","score":2639,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggggctttatatccct
>HELIUM_000100422_612GNAAXX:7:7:7531:15196#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:02)->(C:05)":52,"(C:02)->(G:32)":56},"sample":"13a_F730603","score":1601,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactcccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:89:3988:1696#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":31,"pairing_fast_overlap":62,"pairing_fast_score":0.525,"pairing_mismatches":{"(a:29)->(g:02)":52,"(a:34)->(c:02)":33,"(a:34)->(t:02)":31,"(c:02)->(t:30)":81,"(c:30)->(a:02)":42,"(t:24)->(g:02)":48,"(t:32)->(g:06)":71,"(t:33)->(g:02)":35},"sample":"15a_F730814","score":777,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaagggacttggcggtggtttataccctt
>HELIUM_000100422_612GNAAXX:7:42:5254:21274#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:02)->(T:08)":49,"(T:18)->(G:10)":36,"(T:33)->(G:06)":38},"sample":"15a_F730814","score":908,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagttctaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:55:8649:17296#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"26a_F040644","score":1316,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaaggatgttcgcaagagtactactagcaaca
gcctgaagctcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:17:14547:8595#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"13a_F730603","score":3127,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ctagccttaaacacaaatagttatgcaaacaaactattcgccagagtactaccagcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:109:4439:1480#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(a:34)->(g:02)":31,"(c:11)->(a:29)":66},"sample":"29a_F260619","score":2305,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccctaaacacaagtaattaatataacaaaattattcgccagggtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:120:3226:1948#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(a:02)->(c:34)":79,"(c:02)->(t:31)":80,"(t:02)->(g:20)":64},"sample":"13a_F730603","score":556,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcgtaaaactcaaaggacttggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:58:19781:20538#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(A:02)->(G:09)":67,"(C:32)->(A:02)":42,"(G:27)->(C:02)":41},"sample":"13a_F730603","score":414,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttagaactcaaagggcttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:23:10869:10753#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:02)->(G:10)":52},"sample":"13a_F730603","score":1622,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagtcttaaacacaaatagttatgcaaacaaaactattcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:108:10111:9078#0/1_sub[28..127] {"ali_dir":"left","count":7146,"experiment":"wolf_diet","merged_sample":{"13a_F730603":7146},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:9:12177:12899#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":11,"experiment":"wolf_diet","merged_sample":{"15a_F730814":8,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcggcagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:120:6924:19049#0/1_sub[28..127] {"ali_dir":"left","count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"sample":"15a_F730814","seq_ab_match":60}
ttagccctaaacacaagtaattaacataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:10:13591:16078#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":63,"pairing_fast_score":0.917,"pairing_mismatches":{"(a:02)->(g:34)":76},"sample":"26a_F040644","score":1245,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgcttcacatccct
>HELIUM_000100422_612GNAAXX:7:36:4630:21095#0/1_sub[28..126] {"ali_dir":"left","count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataaacattcaataaacaagaatgtacgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:95:4896:20986#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattactataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:8:10649:15896#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggatttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:108:1408:5205#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(a:02)->(c:34)":63,"(a:08)->(c:02)":42,"(a:10)->(c:02)":40,"(g:02)->(t:34)":81},"sample":"13a_F730603","score":734,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattagacagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:92:11785:4442#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3714,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataagcattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcagtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:28:8535:16868#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"29a_F260619","score":3698,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:82:17775:19300#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":63,"pairing_fast_score":0.6,"pairing_mismatches":{"(a:02)->(c:25)":42,"(a:02)->(g:34)":76,"(c:02)->(a:17)":41,"(c:02)->(a:34)":58,"(c:02)->(g:33)":65,"(c:02)->(g:34)":75,"(c:02)->(t:32)":47,"(c:02)->(t:34)":79,"(g:02)->(c:34)":78},"sample":"26a_F040644","score":551,"score_norm":0.841,"seq_a_single":45,"seq_ab_match":53,"seq_b_single":45}
ttagccctaaacatacacattcaataaacaagaatgttcgacagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:59:19791:18286#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":63,"pairing_fast_score":0.917,"pairing_mismatches":{"(c:27)->(t:07)":39,"(t:34)->(g:14)":38},"sample":"13a_F730603","score":3281,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
tagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:44:8162:3761#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(A:02)->(T:06)":49,"(A:02)->(T:20)":52,"(G:02)->(T:16)":55},"sample":"15a_F730814","score":854,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagttcttcctgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:59:15601:2204#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":63,"pairing_fast_score":0.683,"pairing_mismatches":{"(A:25)->(G:02)":51,"(A:34)->(G:02)":34,"(T:27)->(G:02)":38},"sample":"26a_F040644","score":845,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgcttgacatccct
>HELIUM_000100422_612GNAAXX:7:82:18362:4871#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:02)->(G:11)":49,"(C:30)->(A:09)":42,"(T:32)->(G:02)":25},"sample":"29a_F260619","score":404,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctg
>HELIUM_000100422_612GNAAXX:7:120:19262:16019#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":33,"pairing_fast_overlap":63,"pairing_fast_score":0.55,"pairing_mismatches":{"(A:02)->(C:06)":57,"(A:02)->(G:08)":51,"(A:29)->(G:02)":27,"(A:30)->(T:09)":33,"(C:34)->(G:18)":41,"(T:02)->(G:16)":53},"sample":"26a_F040644","score":855,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactgcgagccaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:3:12234:14799#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:21)->(G:09)":29,"(C:02)->(T:34)":65,"(G:02)->(T:09)":55},"sample":"13a_F730603","score":657,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaagactattcgccagagtactacctgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:93:6991:1942#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(a:10)->(g:34)":76,"(c:06)->(a:34)":58},"sample":"26a_F040644","score":2887,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagaactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:2:3479:10632#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:02)->(C:14)":61,"(C:02)->(G:15)":56,"(T:02)->(G:10)":60},"sample":"29a_F260619","score":366,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaag
cgcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:118:2719:7202#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":11,"experiment":"wolf_diet","merged_sample":{"26a_F040644":11},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagggtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:119:8691:15994#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcgat
agcttaaaacgcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:14:8730:13490#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":32,"pairing_fast_overlap":62,"pairing_fast_score":0.542,"pairing_mismatches":{"(A:02)->(G:07)":49,"(C:02)->(G:14)":53,"(G:02)->(T:05)":56,"(T:02)->(A:10)":59,"(T:29)->(G:23)":38},"sample":"13a_F730603","score":1324,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctagcgtcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:52:4499:13385#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(c:02)->(a:34)":74,"(g:02)->(t:34)":65,"(t:02)->(a:13)":35},"sample":"15a_F730814","score":326,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtgattaatataacaaaaatattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:70:7123:4143#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:02)->(T:33)":39,"(G:02)->(A:25)":69},"sample":"15a_F730814","score":448,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
taagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:73:11944:1830#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcagtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:78:19355:19647#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:32)->(T:09)":28,"(G:02)->(T:25)":62,"(T:34)->(A:07)":27},"sample":"15a_F730814","score":1264,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccgccaat
atcttaaaactcaaacgacttggctgtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:76:12991:3807#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":62,"pairing_fast_score":0.593,"pairing_mismatches":{"(a:02)->(t:30)":39,"(c:02)->(a:30)":49,"(c:02)->(a:31)":68,"(c:02)->(t:34)":65,"(t:02)->(g:11)":35},"sample":"29a_F260619","score":216,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagcaattaatataacaaaagtattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:6:1739:11421#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgctagagtactactagcaaca
gcctgacactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:7:17875:18543#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactgctagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:7:10793:12690#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":63,"pairing_fast_score":0.717,"pairing_mismatches":{"(A:02)->(T:05)":51,"(C:02)->(G:15)":41},"sample":"26a_F040644","score":1266,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcggcagagtacttctagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:5:12288:3807#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(T:06)":69,"(T:02)->(G:21)":64},"sample":"13a_F730603","score":444,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcgtaaatctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:13:15639:12348#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":63,"pairing_fast_score":0.95,"pairing_mismatches":{},"sample":"29a_F260619","score":3373,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactactagcaaca
gattaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:83:14921:18416#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(G:02)->(A:10)":40},"sample":"26a_F040644","score":961,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcaccagagtactactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:115:17535:11351#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(C:02)->(G:09)":69},"sample":"26a_F040644","score":1756,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaagtcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:120:5551:16127#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(A:02)->(G:21)":68,"(T:02)->(G:26)":37},"sample":"26a_F040644","score":569,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatggtcgccagagtactactagcaaca
gcctgaagctcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:73:17136:17728#0/1_sub[28..127] {"ali_dir":"left","count":6,"experiment":"wolf_diet","merged_sample":{"13a_F730603":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ctagccttaaacacaaatagttatgcaaacaaaattattcgccagagtactaccggcaac
agcccaaaactcaaaggacttggcggtgcttcacaccctt
>HELIUM_000100422_612GNAAXX:7:109:1305:18519#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(A:02)->(C:14)":54,"(A:02)->(G:08)":58,"(A:02)->(G:29)":69,"(A:08)->(G:02)":32,"(C:02)->(G:14)":56},"sample":"29a_F260619","score":247,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcgat
agcttaaagctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:19:10619:19798#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":10,"experiment":"wolf_diet","merged_sample":{"15a_F730814":6,"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatacgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:76:9874:3117#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":9,"experiment":"wolf_diet","merged_sample":{"29a_F260619":9},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:84:5672:3853#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":8,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3,"29a_F260619":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttacaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:60:18064:19030#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(c:15)->(a:25)":69,"(t:22)->(a:02)":59,"(t:33)->(a:02)":35},"sample":"15a_F730814","score":611,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcatt
agcttaaaactcaaaggactttgcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:107:3221:2939#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":10,"experiment":"wolf_diet","merged_sample":{"26a_F040644":10},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
tcagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:63:4254:3270#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"sample":"15a_F730814","score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaagaacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:96:8243:20095#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(c:15)->(a:20)":58,"(c:25)->(a:09)":68},"sample":"26a_F040644","score":2138,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaacctcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:82:13194:16629#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":31,"pairing_fast_overlap":63,"pairing_fast_score":0.517,"pairing_mismatches":{"(A:02)->(C:25)":69,"(A:31)->(G:02)":25,"(C:02)->(A:22)":66,"(C:02)->(A:27)":54,"(C:19)->(A:32)":31,"(T:16)->(G:18)":38},"sample":"29a_F260619","score":441,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataatgcgccagagaactactagcaaca
gattaaacctcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:45:17560:4454#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"15a_F730814","score":3446,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccaaagtactaccggcaat
agcttaaaactcaaaggacttggcgatgctttataccctt
>HELIUM_000100422_612GNAAXX:7:104:15020:15985#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(a:02)->(c:30)":72,"(a:06)->(c:02)":43,"(c:02)->(t:34)":65,"(c:07)->(a:02)":49,"(c:09)->(a:02)":59,"(g:02)->(a:30)":69},"sample":"13a_F730603","score":136,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgcaagagtcctaccggcact
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:70:13186:10352#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(c:02)->(t:34)":81,"(c:27)->(a:02)":42,"(g:05)->(c:02)":43},"sample":"15a_F730814","score":1552,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgcgagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:40:18035:2498#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":63,"pairing_fast_score":0.65,"pairing_mismatches":{"(A:34)->(C:02)":28,"(C:34)->(A:02)":29,"(T:34)->(A:02)":25,"(T:34)->(G:02)":35},"sample":"26a_F040644","score":1135,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacgtgtcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:106:1343:2725#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":63,"pairing_fast_score":0.567,"pairing_mismatches":{"(a:34)->(g:02)":30,"(c:02)->(t:34)":80,"(c:08)->(g:10)":62,"(c:34)->(g:02)":41,"(t:02)->(c:34)":69,"(t:24)->(c:02)":42,"(t:34)->(c:02)":37},"sample":"26a_F040644","score":988,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgctagagtactactagcaaca
ggctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:38:14204:12725#0/1_sub[28..126] {"ali_dir":"left","count":87,"experiment":"wolf_diet","merged_sample":{"26a_F040644":87},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:115:14734:13932#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactcgcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:88:1454:7056#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":27,"experiment":"wolf_diet","merged_sample":{"26a_F040644":27},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:18:16679:15889#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:26:1285:7395#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"13a_F730603":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaacccaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:37:7732:19414#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatacaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:71:13208:4118#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctgtataccctt
>HELIUM_000100422_612GNAAXX:7:104:19324:15970#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(c:02)->(a:29)":69,"(t:02)->(g:20)":64,"(t:31)->(g:02)":39},"sample":"13a_F730603","score":368,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcgtaaaactcaaaggacttggcggtgctgtataccctt
>HELIUM_000100422_612GNAAXX:7:52:6908:8410#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataagctattctataacaaaataattcgccagagaactactagcaaca
gcttaaaactcaaaggacttggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:34:13559:8594#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":63,"pairing_fast_score":0.6,"pairing_mismatches":{"(C:02)->(G:23)":62,"(G:02)->(C:31)":69,"(G:02)->(T:09)":61,"(G:34)->(T:02)":40,"(T:34)->(A:02)":25},"sample":"26a_F040644","score":744,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
tgctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:25:12750:4181#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"13a_F730603":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgctagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:71:13461:7411#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagaagactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:41:14359:6582#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(A:21)->(T:18)":22,"(T:32)->(A:15)":21,"(T:32)->(G:10)":35},"sample":"29a_F260619","score":783,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaaataataacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:82:15275:9377#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:02)->(G:10)":49,"(C:02)->(A:32)":67},"sample":"29a_F260619","score":1218,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat
agcttaaagctcaaaggactgggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:84:9576:6338#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtgcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:63:5399:1187#0/1_sub[28..128] {"ali_dir":"left","ali_length":61,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":61,"pairing_fast_score":0.845,"pairing_mismatches":{"(c:02)->(t:34)":65,"(c:15)->(t:23)":48},"sample":"13a_F730603","score":2122,"score_norm":0.951,"seq_a_single":47,"seq_ab_match":58,"seq_b_single":47}
ctagccttaaacacaaataattacacaaacaaaattgttcgccagagtactagcggcaac
agcttaaaactcaaaaggacttagcggtgctttatatcctt
>HELIUM_000100422_612GNAAXX:7:97:15874:5643#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"26a_F040644","score":3031,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacagtcaataaacaaggatgttcgccagagtactactagcagca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:117:7781:7096#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(C:02)->(T:34)":65},"sample":"29a_F260619","score":1722,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttttaccctt
>HELIUM_000100422_612GNAAXX:7:18:9949:9646#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(a:02)->(c:29)":63,"(a:06)->(c:02)":40,"(a:08)->(t:02)":48,"(a:33)->(c:02)":31,"(c:02)->(a:23)":68},"sample":"13a_F730603","score":144,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattagccagagaactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:104:4460:12230#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(c:02)->(a:33)":59},"sample":"13a_F730603","score":678,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaagactattcgccagaggactactagcaat
agcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:118:19502:10502#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":63,"pairing_fast_score":0.567,"pairing_mismatches":{"(a:23)->(t:02)":33,"(a:31)->(g:02)":30,"(c:02)->(a:31)":74,"(c:02)->(a:32)":77,"(c:07)->(t:02)":38,"(c:30)->(a:08)":67},"sample":"26a_F040644","score":454,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
tcagccctaaacataaacattcaataaacaagaatgtccgccagagtactactagcaaca
gcctgacactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:109:3394:1948#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(A:02)->(T:34)":65,"(A:14)->(T:02)":39,"(C:02)->(A:31)":59,"(T:07)->(A:02)":24,"(T:29)->(A:02)":21,"(T:34)->(G:02)":25},"sample":"29a_F260619","score":693,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattatacgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:68:10909:8875#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(C:06)->(G:31)":56},"sample":"29a_F260619","score":2521,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagcccaaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:81:11211:1294#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccgacaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:119:9330:4596#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcagacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:107:12463:15953#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":15,"experiment":"wolf_diet","merged_sample":{"26a_F040644":15},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaagctcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:55:14812:10754#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaatagacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:108:2009:1402#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(A:02)->(G:07)":49,"(A:30)->(C:02)":28,"(A:33)->(G:02)":33,"(C:02)->(A:17)":59,"(T:34)->(A:02)":21},"sample":"15a_F730814","score":195,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat
agcttaaaactcaaaggatttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:106:11105:5936#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"15a_F730814":6,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacaggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:74:15149:15543#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(C:02)->(A:32)":59,"(C:02)->(A:34)":67,"(C:02)->(T:34)":65,"(C:07)->(G:07)":39,"(C:34)->(A:02)":30},"sample":"15a_F730814","score":1140,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatscgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:59:18867:1292#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(a:02)->(c:34)":79,"(a:06)->(t:02)":51,"(c:02)->(g:34)":77,"(c:14)->(a:18)":59,"(c:14)->(a:30)":61,"(t:16)->(g:02)":35},"sample":"29a_F260619","score":488,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagcaattattataacaaaattattcgccagagtacaaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:81:8186:3652#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(c:10)->(g:16)":66},"sample":"29a_F260619","score":2537,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttgaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:84:15960:2191#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(c:02)->(a:31)":69},"sample":"15a_F730814","score":2584,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaaataatataacaaaattattcgccagagtactatcggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:114:16193:1846#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(c:34)->(a:02)":41},"sample":"26a_F040644","score":1244,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgttttatatccct
>HELIUM_000100422_612GNAAXX:7:60:9143:10595#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":63,"pairing_fast_score":0.983,"pairing_mismatches":{},"sample":"26a_F040644","score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagagggctactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:53:9784:13276#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(C:02)->(A:34)":59,"(C:07)->(T:34)":21},"sample":"13a_F730603","score":1777,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatcgttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:55:11038:6117#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3816,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaaaattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:50:15740:5461#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:31)->(G:15)":29,"(T:33)->(G:07)":35},"sample":"15a_F730814","score":1405,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggcgctttataccctt
>HELIUM_000100422_612GNAAXX:7:13:15177:5668#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":21,"pairing_fast_overlap":62,"pairing_fast_score":0.356,"pairing_mismatches":{"(A:02)->(C:25)":54,"(A:02)->(T:24)":65,"(A:15)->(C:02)":24,"(A:18)->(G:30)":28,"(C:09)->(A:30)":32,"(C:20)->(G:07)":39,"(C:29)->(A:14)":42,"(G:02)->(T:07)":55,"(T:02)->(A:19)":68,"(T:02)->(C:16)":57,"(T:32)->(A:02)":21},"sample":"15a_F730814","score":409,"score_norm":0.806,"seq_a_single":46,"seq_ab_match":50,"seq_b_single":46}
ttagccctaaccacaagtaattaatatgacaaaattatccgccagagtactacctgcaat
agcttaaaactcaaaggacttcgcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:30:9772:13523#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(A:32)->(G:02)":23,"(C:02)->(T:34)":65,"(G:02)->(C:23)":55,"(T:02)->(G:07)":39},"sample":"15a_F730814","score":337,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatgcgccagagtactacccgcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:98:16207:5869#0/1_sub[78..81] {"ali_dir":"right","count":2007,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2007},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"sample":"29a_F260619"}
tttt
>HELIUM_000100422_612GNAAXX:7:7:9773:5107#0/1_sub[28..126] {"ali_dir":"left","count":77,"experiment":"wolf_diet","merged_sample":{"26a_F040644":77},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactgctagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:104:6086:21264#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(c:02)->(a:34)":67,"(n:02)->(a:23)":49,"(n:02)->(a:30)":52,"(n:02)->(a:32)":46,"(n:02)->(c:30)":50,"(n:02)->(g:28)":47,"(n:02)->(g:32)":45,"(n:02)->(t:18)":51,"(n:02)->(t:30)":48},"sample":"15a_F730814","score":1220,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttggccctaaacacaggtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:41:2602:12928#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
ggcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:94:11241:2652#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:34)->(G:21)":46,"(G:02)->(A:23)":55},"sample":"29a_F260619","score":1683,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccagcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:116:6287:17844#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(G:02)->(T:10)":41,"(T:02)->(C:11)":51},"sample":"15a_F730814","score":1197,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatatagcaaaattattctccagagtaccaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:72:6179:9538#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacatggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:46:14852:3420#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggccttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:22:4787:10960#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":63,"pairing_fast_score":0.6,"pairing_mismatches":{"(A:02)->(G:09)":58,"(A:32)->(G:02)":24,"(C:18)->(A:02)":28,"(C:34)->(A:02)":22,"(T:02)->(G:20)":50,"(T:34)->(A:02)":20},"sample":"26a_F040644","score":577,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacataaacattcaataaccaagaatgttcgccagagtacgactagcagca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:99:10069:20699#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":27,"pairing_fast_overlap":62,"pairing_fast_score":0.458,"pairing_mismatches":{"(C:02)->(A:20)":69,"(C:02)->(A:34)":67,"(C:07)->(G:21)":56,"(C:28)->(G:02)":42,"(G:17)->(C:02)":41,"(T:02)->(G:16)":71,"(T:18)->(G:23)":48,"(T:32)->(G:02)":22,"(T:34)->(A:02)":25},"sample":"29a_F260619","score":711,"score_norm":0.823,"seq_a_single":46,"seq_ab_match":51,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagaggactaccggcaat
agcttaaaacgcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:83:1773:4951#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(a:02)->(c:32)":72,"(a:02)->(g:09)":69,"(a:02)->(g:32)":77},"sample":"29a_F260619","score":680,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaagctcaaaggacttggcgatgctttataccctt
>HELIUM_000100422_612GNAAXX:7:87:13701:11209#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(a:34)->(g:02)":32,"(g:02)->(c:02)":70},"sample":"13a_F730603","score":14,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaastcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:91:3697:12219#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(A:02)->(G:17)":54},"sample":"26a_F040644","score":1303,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactggcaaca
gcctgaaactcaaaggacttggcggtgttttacatccct
>HELIUM_000100422_612GNAAXX:7:115:11299:18312#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(a:02)->(c:02)":40,"(c:02)->(t:33)":81,"(c:02)->(t:34)":65},"sample":"15a_F730814","score":12,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattmgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:92:4704:1882#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(a:10)->(c:02)":52,"(c:25)->(a:33)":73},"sample":"26a_F040644","score":1475,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactaatagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:96:19089:16041#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":32,"pairing_fast_overlap":63,"pairing_fast_score":0.533,"pairing_mismatches":{"(C:02)->(A:31)":68,"(C:34)->(G:02)":22,"(G:02)->(A:17)":55,"(T:18)->(G:29)":35,"(T:23)->(G:07)":38,"(T:33)->(G:02)":20},"sample":"26a_F040644","score":954,"score_norm":0.857,"seq_a_single":45,"seq_ab_match":54,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaaggttcgccagagtactactaacaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:53:3504:1354#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":63,"pairing_fast_score":0.75,"pairing_mismatches":{"(a:34)->(t:02)":33,"(c:02)->(t:34)":80,"(c:17)->(a:02)":45,"(c:32)->(a:02)":41,"(g:34)->(t:02)":32},"sample":"26a_F040644","score":1674,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagcgtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:84:19360:15853#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":63,"pairing_fast_score":0.6,"pairing_mismatches":{"(A:26)->(G:02)":26,"(A:27)->(G:02)":43,"(A:34)->(C:02)":28,"(C:26)->(A:11)":58,"(C:34)->(G:02)":29},"sample":"26a_F040644","score":72,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcacca
gcctgaaactcaaagtacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:47:2641:2767#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(C:02)->(A:33)":58},"sample":"26a_F040644","score":1298,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggcgctttacatccct
>HELIUM_000100422_612GNAAXX:7:70:3974:10779#0/1_sub[28..127] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":63,"pairing_fast_score":0.783,"pairing_mismatches":{"(C:10)->(G:27)":56,"(C:14)->(A:28)":59,"(C:21)->(G:25)":54,"(T:25)->(G:24)":39},"sample":"29a_F260619","score":2354,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacgggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:42:16736:2480#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":63,"pairing_fast_score":0.567,"pairing_mismatches":{"(A:34)->(C:02)":23,"(A:34)->(G:02)":31,"(C:35)->(A:02)":22,"(G:33)->(T:02)":36,"(G:34)->(A:02)":32,"(T:22)->(G:12)":53,"(T:34)->(A:02)":25,"(T:34)->(G:02)":35},"sample":"26a_F040644","score":175,"score_norm":0.841,"seq_a_single":45,"seq_ab_match":53,"seq_b_single":45}
ttagccctaaacataagcattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:64:13700:17615#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":32,"pairing_fast_overlap":62,"pairing_fast_score":0.542,"pairing_mismatches":{"(A:26)->(G:20)":34,"(A:32)->(G:08)":46,"(C:02)->(G:07)":57,"(C:02)->(G:10)":56,"(T:02)->(G:16)":48,"(T:02)->(G:24)":51},"sample":"13a_F730603","score":751,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagaggacgaccgggaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:50:5172:18320#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(G:02)->(T:10)":62},"sample":"15a_F730814","score":881,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
atcttaaaactcaaaggacttggcggcgctttataccctt
>HELIUM_000100422_612GNAAXX:7:97:5079:15960#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":63,"pairing_fast_score":0.95,"pairing_mismatches":{},"sample":"26a_F040644","score":3492,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaaccattctataacaaaataattcgccagagaactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:7:18055:4363#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":19,"experiment":"wolf_diet","merged_sample":{"13a_F730603":19},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactatccgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(A:02)->(G:23)":49,"(C:02)->(A:33)":59,"(C:02)->(G:33)":56,"(T:24)->(A:21)":35},"sample":"29a_F260619","score":1609,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttatacgctt
>HELIUM_000100422_612GNAAXX:7:58:13909:7316#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(A:09)->(C:02)":53,"(A:22)->(G:02)":57},"sample":"26a_F040644","score":534,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacaagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:103:11671:4782#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagaactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:47:6989:13864#0/1_sub[28..126] {"ali_dir":"left","count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_mismatches":{},"sample":"26a_F040644","score_norm":0.984}
ttagccctaaacataaaccattctataacaaaataattcgccagagaactactagcaaca
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:110:17423:14909#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccgccaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:114:13578:18434#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(C:02)->(G:09)":42,"(C:02)->(G:34)":56,"(C:02)->(T:19)":48,"(C:21)->(A:02)":24,"(T:24)->(G:23)":35},"sample":"29a_F260619","score":77,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattactataacaaaattattcggcagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:65:7341:13598#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agctttaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:37:10373:13876#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(c:02)->(a:31)":73,"(c:02)->(t:31)":80,"(c:10)->(t:28)":47,"(c:17)->(a:32)":31},"sample":"26a_F040644","score":1867,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacacaaacattcaataaacaagaatgttcgccagagtactactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:66:2848:7484#0/1_sub[28..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":64,"pairing_fast_score":0.869,"pairing_mismatches":{"(t:33)->(c:07)":38,"(t:34)->(g:02)":35},"sample":"26a_F040644","score":2312,"score_norm":0.953,"seq_a_single":44,"seq_ab_match":61,"seq_b_single":44}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:75:6465:13395#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgmcagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:69:1724:16634#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":16,"experiment":"wolf_diet","merged_sample":{"15a_F730814":12,"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccggagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:105:7990:5630#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaatcaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:58:8807:1247#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"pairing_mismatches":{},"sample":"26a_F040644","score":3218,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gactgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:95:17788:6129#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3,"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacctggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:31:1735:7805#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":33,"pairing_fast_overlap":62,"pairing_fast_score":0.559,"pairing_mismatches":{"(a:02)->(g:31)":77,"(a:02)->(t:18)":51,"(c:02)->(a:18)":74,"(c:02)->(a:32)":67,"(c:02)->(g:32)":56,"(t:02)->(g:09)":80,"(t:18)->(g:02)":35},"sample":"29a_F260619","score":65,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagcaattaatataacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:44:2411:13640#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"13a_F730603":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcggcagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:2:4711:16056#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":63,"pairing_fast_score":0.983,"pairing_mismatches":{},"sample":"26a_F040644","score":845,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagagtactactagcaata
gcttaaaactcaaaggacttggcggtgctttacagccct
>HELIUM_000100422_612GNAAXX:7:83:15749:6663#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:20)->(G:11)":34,"(C:02)->(A:34)":67,"(T:02)->(G:16)":51,"(T:17)->(G:32)":39},"sample":"13a_F730603","score":1390,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactatgcgccagagtacgaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:72:8941:18482#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaatg
gcctaaaactcaaaggacttggtggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:37:1991:3577#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(C:02)->(A:07)":69,"(C:02)->(G:09)":70,"(C:02)->(G:34)":56},"sample":"29a_F260619","score":1512,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaagtcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:96:8516:2664#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"sample":"13a_F730603","score":1990,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctacccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:64:8656:19252#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3289,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaaaataattcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:45:10882:15771#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"sample":"15a_F730814","score":1906,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacggggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:108:3837:12693#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{},"sample":"29a_F260619","score":1463,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactactagcaaca
gattaaacctcaaaggacttgtcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:56:1679:20868#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgcttgataccctt
>HELIUM_000100422_612GNAAXX:7:14:14762:14485#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(a:09)->(c:32)":72,"(t:31)->(a:06)":35},"sample":"29a_F260619","score":2800,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacgcaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaagctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:28:3969:15209#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaagactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":63,"pairing_fast_score":0.7,"pairing_mismatches":{"(A:02)->(G:08)":66,"(T:02)->(G:09)":70,"(T:34)->(G:02)":20},"sample":"26a_F040644","score":193,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctggaacgcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:74:12517:10397#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcgaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:13:11410:10890#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":10,"experiment":"wolf_diet","merged_sample":{"26a_F040644":10},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccaaagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:17:3035:4831#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{},"sample":"29a_F260619","score":1578,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
tcagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcgaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:25:10818:13742#0/1_sub[28..133] {"ali_dir":"left","ali_length":56,"count":8,"experiment":"wolf_diet","merged_sample":{"26a_F040644":8},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":56,"sample":"26a_F040644","seq_a_single":52,"seq_b_single":52}
ttagccctaaacgtaaactgcaaactattccataataaaataattcgcccaagaactact
agcaacagcttaaaactcaaaggacttggtggtgctttctacccct
>HELIUM_000100422_612GNAAXX:7:3:10582:8570#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":63,"pairing_fast_score":0.75,"pairing_mismatches":{"(a:02)->(c:32)":63,"(c:02)->(a:32)":68,"(c:02)->(g:16)":62},"sample":"26a_F040644","score":97,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaccaagaatgttcgccagagtactactagcaaca
ggctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:106:18178:3009#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(C:02)->(G:10)":42,"(C:02)->(G:13)":56},"sample":"15a_F730814","score":609,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcggcagagtactaccggcaat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:117:5948:7120#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttgaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:47:2502:16294#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagtcctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:60:9112:14337#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaagcacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:114:9937:9668#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(c:02)->(a:34)":74,"(c:02)->(t:34)":81,"(c:15)->(g:02)":56,"(t:29)->(g:02)":35},"sample":"15a_F730814","score":895,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccgccaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:6:9266:16064#0/1_sub[28..127] {"ali_dir":"left","count":10,"experiment":"wolf_diet","merged_sample":{"15a_F730814":6,"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0}
ttagccctaaacgcaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:85:4839:3905#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"26a_F040644","score":1339,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:5:1399:6458#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(C:02)->(A:33)":59,"(T:18)->(G:18)":39},"sample":"13a_F730603","score":1433,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactatkcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:25:9692:10209#0/1_sub[28..127] {"ali_dir":"left","count":5,"experiment":"wolf_diet","merged_sample":{"13a_F730603":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603","score_norm":0.984}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggtggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:96:12491:8972#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacgaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:37:2595:1170#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(G:10)->(C:16)":40},"sample":"26a_F040644","score":1625,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagtcctaaacataaacattcaataaacaagaatgttccccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:82:9331:3023#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(C:02)->(A:31)":69,"(C:17)->(T:02)":36,"(T:29)->(G:02)":35},"sample":"29a_F260619","score":663,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaatcattcgccagagtactaccggcaat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:39:19114:20117#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":63,"pairing_fast_score":0.817,"pairing_mismatches":{"(G:02)->(T:13)":61,"(T:34)->(A:02)":21},"sample":"26a_F040644","score":1678,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
tcctaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:64:11561:14364#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(C:02)->(G:06)":53,"(T:02)->(G:07)":51},"sample":"15a_F730814","score":1782,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtacgagcggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:28:8017:12717#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":62,"pairing_fast_score":0.949,"pairing_mismatches":{"(g:02)->(t:34)":81},"sample":"15a_F730814","score":2276,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcaccagagtactaccggcaat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:65:16979:18377#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":63,"pairing_fast_score":0.7,"pairing_mismatches":{"(C:02)->(A:30)":68,"(C:02)->(G:20)":41,"(T:02)->(G:15)":37},"sample":"26a_F040644","score":430,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatggtcggcagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:70:11509:6042#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagaaattaatataacaaaaatattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:30:9942:4495#0/1_sub[28..126] {"ali_dir":"left","count":95,"experiment":"wolf_diet","merged_sample":{"26a_F040644":11,"29a_F260619":84},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0}
ttagccctaaacataagctattccataacaaaataattcgccagagaactactagcaaca
gattaaacctcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:119:9582:19349#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":7,"experiment":"wolf_diet","merged_sample":{"26a_F040644":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgtcagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:107:16154:20228#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":62,"pairing_fast_score":0.593,"pairing_mismatches":{"(A:02)->(G:12)":49,"(A:02)->(G:14)":29,"(C:02)->(A:22)":59,"(G:02)->(T:10)":55,"(T:02)->(G:23)":22},"sample":"13a_F730603","score":28,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ctagccttaaacacaaatagtgatgcaagcaaaactattcgccagagtgctacctgcaat
agcttaaaactcaaagtacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:114:4438:10974#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(C:02)->(T:06)":56,"(C:02)->(T:30)":65,"(C:28)->(G:18)":50,"(T:30)->(G:20)":38},"sample":"29a_F260619","score":705,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcgtcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:32:10356:2372#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(A:02)->(C:24)":54,"(A:02)->(T:16)":49,"(T:02)->(G:16)":71,"(T:10)->(G:12)":36,"(T:10)->(G:16)":39},"sample":"15a_F730814","score":394,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaatgatgcgccagagttctaccggcaat
agcttaaaacgcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:13:2815:5012#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatgcct
>HELIUM_000100422_612GNAAXX:7:109:18788:16169#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaagtgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:110:17902:19766#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcgccaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:62:15459:19744#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(a:26)->(g:27)":58,"(c:02)->(t:34)":81,"(c:07)->(a:32)":59,"(c:20)->(a:31)":69,"(c:22)->(t:34)":65,"(t:24)->(g:32)":64},"sample":"15a_F730814","score":1401,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcgat
agcgtaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:46:4342:17317#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaatcattctataacaaaataattcgccggagaactactaggaaca
gcttaaaactcaaaggacttggcggtgccttacgtccct
>HELIUM_000100422_612GNAAXX:7:98:16267:16369#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:21)->(G:02)":52,"(C:21)->(G:02)":50,"(T:27)->(G:02)":36},"sample":"29a_F260619","score":40,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaagggcttggcggtgctttattccctt
>HELIUM_000100422_612GNAAXX:7:75:18428:11763#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"seq_a_single":45,"seq_b_single":45}
ttagccctaaacacaagtaattaatatacaaaattattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:107:15352:1099#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(C:02)->(A:20)":59,"(C:02)->(A:33)":67,"(C:02)->(T:33)":65,"(T:02)->(G:12)":51,"(T:16)->(A:10)":38},"sample":"15a_F730814","score":1372,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtacgaccggcaat
agcttaaaactcaaaggccttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:116:12749:12270#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(a:27)->(c:24)":43,"(t:33)->(g:06)":35},"sample":"29a_F260619","score":3251,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaaatgattacacaaacaaaattgttcacaagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:67:7784:4802#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:02)->(T:08)":52,"(A:02)->(T:13)":49,"(C:02)->(G:07)":53,"(G:02)->(C:15)":54,"(T:25)->(A:11)":24},"sample":"13a_F730603","score":499,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagttcttgcggcaat
agcttaaaactcaaagtgcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:14:19739:17759#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaacctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:89:16257:2583#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(a:34)->(g:02)":32,"(c:28)->(a:28)":75,"(t:29)->(g:02)":35},"sample":"15a_F730814","score":393,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaamggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:6:11980:16222#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(A:29)->(G:13)":31,"(C:02)->(G:10)":42,"(C:02)->(G:24)":56,"(G:02)->(T:11)":55,"(T:31)->(A:17)":21},"sample":"29a_F260619","score":819,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatatgacaaaattattcagcagagtactacctgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:119:8165:12959#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(a:34)->(t:02)":33,"(c:11)->(t:02)":47},"sample":"26a_F040644","score":711,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagcactactagcaaca
gcctgaaactcaaaggacttgacggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:111:17844:17230#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":13,"experiment":"wolf_diet","merged_sample":{"26a_F040644":13},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaatcagtctataacaaaataattcgccagagaactactagcaaca
gcttaaaactcaaaggacgtggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:22:19215:4162#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttacccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:102:10271:1869#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":8,"experiment":"wolf_diet","merged_sample":{"15a_F730814":5,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatgtaacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:55:7856:13124#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactaaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:36:5536:3657#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
tcagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:117:6592:7203#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgcaagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:24:8240:18658#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgccttataccctt
>HELIUM_000100422_612GNAAXX:7:56:8381:16038#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(T:23)->(G:18)":35},"sample":"29a_F260619","score":807,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
tcagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggccttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:34:17680:16952#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":15,"experiment":"wolf_diet","merged_sample":{"13a_F730603":15},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgcttcacaccctt
>HELIUM_000100422_612GNAAXX:7:47:3523:5665#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(a:33)->(g:09)":52,"(c:14)->(a:34)":67,"(c:17)->(a:30)":32,"(g:02)->(t:30)":81},"sample":"29a_F260619","score":3026,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaaataattacacacacaaaattgttcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:113:8334:20321#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"sample":"29a_F260619","score":1939,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaataattcgccagagaactactagcaac
agattaaacctcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:12:6438:18474#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattgatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:48:17600:16606#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(T:34)->(G:02)":25},"sample":"29a_F260619","score":142,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaagcacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggactgggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:108:11923:13475#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":63,"pairing_fast_score":0.783,"pairing_mismatches":{"(A:02)->(G:07)":51,"(T:02)->(G:18)":53},"sample":"26a_F040644","score":267,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgctgcgagcaaca
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:14:9416:19823#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(C:02)->(G:09)":42},"sample":"15a_F730814","score":719,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcggcagagtactaccggcaat
agcttagaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:105:13670:14357#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(c:18)->(t:02)":48,"(c:28)->(a:21)":74,"(t:27)->(c:02)":38},"sample":"13a_F730603","score":1241,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagcactaccggcaat
agcttaaaactcacaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:107:8317:20887#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(A:02)->(G:10)":52,"(C:09)->(A:02)":33,"(T:23)->(G:02)":21},"sample":"13a_F730603","score":477,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaacactattcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:35:15881:19297#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(A:07)->(C:31)":63,"(A:34)->(G:02)":46,"(G:06)->(A:17)":56,"(T:34)->(A:02)":27},"sample":"29a_F260619","score":839,"score_norm":0.839,"seq_a_single":46,"seq_ab_match":52,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccgacaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:27:10336:14847#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":63,"pairing_fast_score":0.717,"pairing_mismatches":{"(A:28)->(G:18)":30,"(C:02)->(G:23)":49,"(G:02)->(C:34)":63},"sample":"26a_F040644","score":713,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtactagcaaca
gcctgaaactcaaagggcttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:102:2995:21011#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(A:02)->(C:30)":54,"(A:02)->(G:07)":52,"(A:34)->(G:02)":23,"(T:13)->(G:27)":39,"(T:20)->(G:25)":35,"(T:30)->(A:02)":22},"sample":"15a_F730814","score":965,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtatgcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:43:2111:20465#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":26,"experiment":"wolf_diet","merged_sample":{"13a_F730603":26},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:94:10057:19854#0/1_sub[28..126] {"ali_dir":"left","count":8,"experiment":"wolf_diet","merged_sample":{"26a_F040644":8},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataaacattcactaaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:93:8014:15314#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:02)->(G:08)":49,"(A:33)->(G:18)":32,"(T:02)->(G:08)":38},"sample":"15a_F730814","score":784,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattagtcgccagagtgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:62:16632:12812#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(C:02)->(T:33)":65,"(T:33)->(A:02)":25,"(T:33)->(G:34)":36,"(T:34)->(G:20)":48},"sample":"29a_F260619","score":1863,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagcaattaatataacaaaatgattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:34:2640:2539#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":19,"experiment":"wolf_diet","merged_sample":{"29a_F260619":19},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:118:14245:17216#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":28,"pairing_fast_overlap":62,"pairing_fast_score":0.475,"pairing_mismatches":{"(A:02)->(G:18)":69,"(A:02)->(G:19)":58,"(C:02)->(G:20)":42,"(C:02)->(G:34)":56,"(T:02)->(G:22)":39,"(T:32)->(G:02)":27,"(T:34)->(A:02)":21},"sample":"15a_F730814","score":370,"score_norm":0.823,"seq_a_single":46,"seq_ab_match":51,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatgcggcagagtactaccggcgat
agcttaaagctcagaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:58:9204:20158#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":2255,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactatttgccagagtactaccggcaat
agcttaaaactcaaagtacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:21:10843:15862#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:09)->(T:02)":36,"(C:02)->(T:34)":65,"(C:14)->(A:02)":33},"sample":"15a_F730814","score":1073,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaacataattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:91:13867:20722#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:25)->(G:07)":34,"(T:10)->(G:13)":36,"(T:20)->(G:21)":48},"sample":"29a_F260619","score":1450,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaatgattcgccagaggactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:103:1908:4722#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagcctcaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:39:18931:6754#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(C:02)->(T:34)":65},"sample":"29a_F260619","score":1821,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:77:12598:19691#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"15a_F730814","score":3547,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacacaagtaattaatataacaaaattattcgcagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:47:14947:6092#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":32,"pairing_fast_overlap":62,"pairing_fast_score":0.542,"pairing_mismatches":{"(a:33)->(g:02)":34,"(a:34)->(g:22)":67,"(c:34)->(g:02)":30,"(t:33)->(g:14)":71},"sample":"29a_F260619","score":1190,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctgtagaccctt
>HELIUM_000100422_612GNAAXX:7:101:9901:16402#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":63,"pairing_fast_score":0.817,"pairing_mismatches":{"(C:02)->(T:34)":64,"(G:02)->(T:07)":61},"sample":"26a_F040644","score":1640,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
tcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:30:2313:12529#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaatacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:24:6702:9290#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"pairing_mismatches":{"(a:02)->(c:31)":79,"(c:02)->(a:32)":74},"sample":"29a_F260619","score":3627,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgcttcatatccct
>HELIUM_000100422_612GNAAXX:7:118:10305:18508#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":63,"pairing_fast_score":0.583,"pairing_mismatches":{"(a:34)->(g:02)":33,"(c:02)->(t:27)":80,"(c:07)->(a:02)":51,"(c:11)->(g:27)":61,"(c:23)->(a:33)":68,"(c:34)->(g:02)":41,"(t:09)->(c:02)":52},"sample":"26a_F040644","score":732,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactcttagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:40:17437:20291#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:14)->(G:02)":46,"(A:26)->(C:02)":49},"sample":"15a_F730814","score":149,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttagaccctt
>HELIUM_000100422_612GNAAXX:7:42:6619:20583#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3966,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggatttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:85:8279:10569#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":4245,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaaccattctataacaaaataattcgccagagaactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:19:7161:8436#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"26a_F040644","score":1793,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaaagttcaccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:91:16692:2647#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(A:26)->(T:02)":33,"(C:02)->(A:23)":69,"(C:28)->(A:02)":26,"(T:24)->(G:08)":51},"sample":"13a_F730603","score":776,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaatgacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:115:12881:7741#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:31)->(G:10)":32,"(C:02)->(G:33)":56,"(G:02)->(A:18)":55,"(T:14)->(G:14)":36},"sample":"15a_F730814","score":1428,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaatkattcgccagagtactaccagcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:89:15784:12674#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(A:25)->(G:18)":52,"(C:02)->(A:34)":67,"(T:29)->(G:31)":35},"sample":"29a_F260619","score":1973,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaagtattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:9:2180:19755#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":63,"pairing_fast_score":0.7,"pairing_mismatches":{"(A:02)->(G:18)":54,"(A:34)->(N:02)":28,"(C:02)->(G:10)":49,"(C:02)->(G:34)":61},"sample":"26a_F040644","score":157,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtactggcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:81:11278:15492#0/1_sub[28..127] {"ali_dir":"left","count":101,"experiment":"wolf_diet","merged_sample":{"15a_F730814":63,"29a_F260619":38},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:2:10593:17264#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgcgagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:60:16581:16640#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctagacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:47:11329:13481#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggactgggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:57:9321:20590#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(A:12)->(T:10)":39},"sample":"29a_F260619","score":2275,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatacgccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:89:16475:16136#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaataagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:81:10525:11143#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:64:16078:3183#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"13a_F730603":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtactttataccctt
>HELIUM_000100422_612GNAAXX:7:37:6735:14104#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"sample":"13a_F730603","score":1697,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattggccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:2:13507:2431#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:02)->(C:07)":52,"(T:02)->(G:15)":48},"sample":"15a_F730814","score":899,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagaggactcccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:5:2892:3036#0/1_sub[78..81] {"ali_dir":"right","ali_length":58,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":58,"pairing_fast_score":1,"sample":"29a_F260619","score":4036,"score_norm":1,"seq_a_single":50,"seq_ab_match":58,"seq_b_single":50}
tctt
>HELIUM_000100422_612GNAAXX:7:68:1480:14965#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"26a_F040644","score":1973,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:106:9586:15140#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:02)->(G:15)":52,"(T:02)->(G:10)":71},"sample":"15a_F730814","score":1491,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat
agcttaaaacgcaaaggacttggcggtcctttataccctt
>HELIUM_000100422_612GNAAXX:7:116:2504:9182#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(A:22)->(G:23)":61,"(T:34)->(A:02)":21},"sample":"29a_F260619","score":2256,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
ggcttaaaactcaaaggacttggcggtgccttataccctt
>HELIUM_000100422_612GNAAXX:7:101:3027:8331#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":63,"pairing_fast_score":0.75,"pairing_mismatches":{"(A:02)->(G:19)":51,"(G:02)->(T:10)":61,"(T:34)->(A:02)":21},"sample":"26a_F040644","score":1064,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactgctagcaaca
tcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:9:3376:6420#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(n:02)->(c:34)":72},"sample":"13a_F730603","score":2368,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatacttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:3:10295:3334#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(G:15)->(T:15)":55,"(T:34)->(G:02)":21},"sample":"15a_F730814","score":651,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacckgcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:89:13177:17379#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(A:02)->(G:07)":49,"(C:02)->(A:30)":69,"(G:02)->(T:05)":47,"(G:34)->(T:02)":25},"sample":"13a_F730603","score":519,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagattgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:37:13494:12286#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(c:16)->(a:34)":75,"(c:21)->(t:21)":39},"sample":"29a_F260619","score":2902,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatycgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:104:6645:11437#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacacccct
>HELIUM_000100422_612GNAAXX:7:111:9491:17972#0/1_sub[28..127] {"ali_dir":"left","count":68,"experiment":"wolf_diet","merged_sample":{"15a_F730814":18,"29a_F260619":50},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:86:6487:3901#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":8,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4,"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgtcagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:28:16164:11230#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaatkattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:3:10054:20858#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattagtcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:19:16017:4858#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":14,"experiment":"wolf_diet","merged_sample":{"13a_F730603":14},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:47:13430:17021#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctatacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:89:10213:5150#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaccacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:46:2545:4779#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(c:02)->(g:32)":77},"sample":"29a_F260619","score":2829,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcactagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:118:12735:20140#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacgagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:83:18977:19319#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:27:14342:5020#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"29a_F260619","score":3613,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttcgccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:55:8678:8913#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"sample":"13a_F730603","score":1512,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaacagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:102:19226:6041#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(c:02)->(a:34)":68,"(c:02)->(t:33)":65},"sample":"29a_F260619","score":1838,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgctcaccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:71:19429:2964#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(A:02)->(C:25)":63,"(A:02)->(G:13)":58,"(C:02)->(A:15)":59,"(C:02)->(T:12)":39},"sample":"29a_F260619","score":123,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacacgtaattaatataacaaaattattcgccagagtactaccggcgat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:71:16725:1631#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccaaagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:58:19422:6968#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacatacacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:92:6658:12126#0/1_sub[28..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":64,"pairing_fast_score":0.885,"pairing_mismatches":{},"sample":"26a_F040644","score":921,"score_norm":0.969,"seq_a_single":44,"seq_ab_match":62,"seq_b_single":44}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgcttacatccct
>HELIUM_000100422_612GNAAXX:7:118:3356:12668#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(C:02)->(A:26)":69,"(C:02)->(G:27)":56},"sample":"29a_F260619","score":1408,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctg
>HELIUM_000100422_612GNAAXX:7:106:3271:20089#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":63,"pairing_fast_score":0.717,"pairing_mismatches":{"(a:02)->(t:34)":80,"(a:06)->(c:30)":56,"(c:08)->(a:02)":51,"(c:09)->(a:02)":55,"(c:10)->(t:02)":47},"sample":"26a_F040644","score":382,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagcactcctaccaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:13:11471:17488#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(A:02)->(C:32)":63,"(A:02)->(T:34)":48,"(C:02)->(G:23)":56,"(C:15)->(A:30)":20},"sample":"29a_F260619","score":731,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctacacacacgtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:51:10360:14965#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"sample":"13a_F730603","score":2085,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcaccagaggactactagcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:92:16157:9417#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(T:33)->(G:15)":35},"sample":"29a_F260619","score":3284,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaacccaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:10:7689:8944#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"26a_F040644","score":1526,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgctcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgccttacatccct
>HELIUM_000100422_612GNAAXX:7:116:4181:14261#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(a:02)->(t:28)":70,"(c:09)->(t:02)":47,"(c:16)->(t:02)":38},"sample":"26a_F040644","score":204,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtccgccagagcactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:1:17280:20040#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"13a_F730603":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaagtagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:47:8422:15680#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(t:34)->(c:06)":38},"sample":"15a_F730814","score":3802,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactactggcaat
agcttaaaactcaaaggacttggcggtactttataccctt
>HELIUM_000100422_612GNAAXX:7:28:12954:19654#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"29a_F260619":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:56:7914:10663#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaatattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:109:14260:17793#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(C:02)->(A:32)":59,"(C:02)->(T:17)":56,"(C:02)->(T:34)":65,"(G:02)->(T:21)":62,"(T:18)->(G:07)":36},"sample":"15a_F730814","score":1039,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccgtcaat
atcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:88:6095:12972#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaaktaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:114:19121:6376#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(c:02)->(a:34)":74,"(c:02)->(g:34)":77,"(c:02)->(t:34)":81,"(t:33)->(c:02)":38},"sample":"13a_F730603","score":917,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaagcacaaatagttatgcaaacaaagctattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:23:12512:15980#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"26a_F040644","score":1663,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactggcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:66:16653:9134#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgttttataccctt
>HELIUM_000100422_612GNAAXX:7:92:12237:21089#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(G:07)":49,"(C:02)->(A:29)":69,"(C:34)->(G:02)":30,"(G:02)->(A:10)":53},"sample":"29a_F260619","score":799,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtgctaacggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:53:7946:10617#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:02)->(C:32)":54,"(G:18)->(A:18)":28},"sample":"29a_F260619","score":1774,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatatracaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:18944:3220#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(A:02)->(C:10)":51,"(T:34)->(A:02)":20},"sample":"26a_F040644","score":967,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactcctagcaatg
gcctaaaactcaaaggacttggtggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:73:4957:13951#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcataaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:44:15230:12038#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaaagttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:85:6179:10780#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaata
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:21:5236:7070#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(A:02)->(C:34)":42,"(A:02)->(T:07)":61,"(C:02)->(G:29)":41,"(G:02)->(C:22)":54,"(T:02)->(G:14)":60},"sample":"29a_F260619","score":219,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaag
tgcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:16:11705:10512#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(C:02)->(G:27)":56},"sample":"29a_F260619","score":1853,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgccttataccctt
>HELIUM_000100422_612GNAAXX:7:11:9411:6694#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"29a_F260619","score":3217,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaggaacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:2343:18143#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:02)->(T:05)":52},"sample":"29a_F260619","score":556,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtacttccggcaat
agcttaaaactcaaaggacttggcggtgccttataccctt
>HELIUM_000100422_612GNAAXX:7:31:11366:14671#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(c:10)->(g:28)":62},"sample":"15a_F730814","score":927,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtacttaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttagcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:8172:11523#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(a:14)->(c:02)":43,"(g:05)->(a:02)":49,"(t:05)->(a:02)":44},"sample":"13a_F730603","score":1683,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgcatgagtgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:1888:16975#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(c:10)->(t:02)":47,"(c:26)->(a:02)":41,"(g:07)->(c:02)":49,"(t:27)->(g:02)":37},"sample":"26a_F040644","score":683,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagcagtactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:10:16070:5070#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacactagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:71:18891:9661#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaatcagtctataacaaaataattcgccagagaactactagcaaca
gcttaaaactcaaaggacgtggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:35:7796:8741#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(a:02)->(c:30)":54,"(c:02)->(a:31)":68,"(c:02)->(a:34)":75,"(c:02)->(t:29)":38},"sample":"15a_F730814","score":380,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagcaattaatataacaaaattattcgccagagtactaccagcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:53:8236:2171#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":63,"pairing_fast_score":0.717,"pairing_mismatches":{"(A:32)->(G:02)":28,"(C:34)->(G:02)":39,"(G:34)->(A:02)":40,"(T:30)->(G:02)":37},"sample":"26a_F040644","score":340,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggactgtgcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:15:16742:19808#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(G:25)":58,"(A:28)->(G:09)":34},"sample":"13a_F730603","score":1060,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcgat
agcttaaaactcaaaggactgggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:36:10022:5082#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":3333,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatagcctt
>HELIUM_000100422_612GNAAXX:7:26:16553:1613#0/1_sub[77..81] {"ali_dir":"right","count":38,"experiment":"wolf_diet","merged_sample":{"13a_F730603":38},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
caata
>HELIUM_000100422_612GNAAXX:7:32:13510:14461#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":63,"pairing_fast_score":0.883,"pairing_mismatches":{"(A:34)->(G:02)":34},"sample":"26a_F040644","score":1190,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacgtggcggtgctttacacccct
>HELIUM_000100422_612GNAAXX:7:118:10152:18423#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":8,"experiment":"wolf_diet","merged_sample":{"26a_F040644":8},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcccgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:73:4869:6151#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtactttacatccct
>HELIUM_000100422_612GNAAXX:7:45:5732:11220#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggtggtgctttctacccct
>HELIUM_000100422_612GNAAXX:7:31:12013:10776#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
ggcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:67:16437:7992#0/1_sub[78..81] {"ali_dir":"right","ali_length":58,"count":4,"experiment":"wolf_diet","merged_sample":{"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":58,"pairing_mismatches":{},"sample":"29a_F260619","score_norm":0.966,"seq_a_single":50,"seq_ab_match":56,"seq_b_single":50}
gttt
>HELIUM_000100422_612GNAAXX:7:41:18256:13201#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":6,"experiment":"wolf_diet","merged_sample":{"29a_F260619":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"29a_F260619","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactactagcaaca
gattaaacctcaaaggacttggcagtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:1:14254:1103#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":8,"experiment":"wolf_diet","merged_sample":{"26a_F040644":8},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:48:18855:7635#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:02)->(C:34)":54,"(C:02)->(A:35)":59},"sample":"29a_F260619","score":1602,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccagcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:61:14664:2454#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"pairing_mismatches":{},"sample":"26a_F040644","score":2141,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactctaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:119:2511:8424#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(a:09)->(g:02)":41,"(a:11)->(c:02)":43,"(a:34)->(g:02)":31},"sample":"29a_F260619","score":1350,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcacaagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:73:3108:1968#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":63,"pairing_fast_score":0.667,"pairing_mismatches":{"(A:02)->(T:06)":58,"(A:02)->(T:07)":60,"(C:02)->(A:23)":54,"(C:34)->(G:02)":22},"sample":"26a_F040644","score":367,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcatct
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:64:10041:1613#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(C:08)->(A:02)":34,"(T:32)->(G:02)":35},"sample":"29a_F260619","score":1593,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaacttgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:70:17593:17783#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:02)->(C:34)":54,"(A:02)->(G:17)":52,"(A:06)->(C:32)":43},"sample":"29a_F260619","score":925,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcagtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:54:5490:5209#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":63,"pairing_fast_score":0.917,"pairing_mismatches":{"(c:34)->(a:02)":41},"sample":"26a_F040644","score":1646,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcaccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:38:10423:6374#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(T:34)->(G:07)":25},"sample":"26a_F040644","score":3373,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttggccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:6:5561:21179#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(A:14)->(G:19)":32,"(C:02)->(A:26)":59,"(C:02)->(A:30)":69,"(T:02)->(A:32)":67,"(T:30)->(G:09)":38},"sample":"29a_F260619","score":1933,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattattataacagaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:77:5449:16101#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":63,"pairing_fast_score":0.783,"pairing_mismatches":{"(c:02)->(a:30)":75,"(c:02)->(g:27)":62,"(t:26)->(g:02)":35},"sample":"29a_F260619","score":58,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttgcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:101:14725:4165#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(c:35)->(a:02)":41,"(t:33)->(c:02)":37},"sample":"26a_F040644","score":1834,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaagggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:90:8148:16626#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:02)->(T:34)":65,"(C:02)->(A:34)":59,"(C:29)->(G:10)":42},"sample":"29a_F260619","score":2127,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagaactactagcaac
agattaaacctcaaaggacttggcagtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:35:9921:7526#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":63,"pairing_fast_score":0.6,"pairing_mismatches":{"(A:02)->(G:09)":48,"(C:14)->(A:02)":28,"(G:02)->(C:25)":63,"(G:02)->(T:13)":55,"(T:28)->(G:02)":35},"sample":"26a_F040644","score":297,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaccaagaatgttcgccagagtgctactatcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:45:14887:13484#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"sample":"15a_F730814","score":1584,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtatttaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:43:11543:12894#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"sample":"29a_F260619","score":1728,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagggtactatcggcaat
agcttaaaactcaaaggacttggcggtgctttatatcctt
>HELIUM_000100422_612GNAAXX:7:37:2253:17636#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(c:02)->(t:25)":81,"(c:06)->(a:23)":69,"(t:02)->(g:10)":80},"sample":"13a_F730603","score":715,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttattcccgt
>HELIUM_000100422_612GNAAXX:7:103:17280:14253#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"pairing_mismatches":{},"sample":"26a_F040644","score":2665,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaaca
gcctgaaactcaaaggactgggcggtgctttacatcccc
>HELIUM_000100422_612GNAAXX:7:18:14622:14689#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":2398,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataagctattctataacaaaataattcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:7:14405:19348#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":63,"experiment":"wolf_diet","merged_sample":{"15a_F730814":38,"29a_F260619":25},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:1:12207:14400#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagctctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:108:2855:5985#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":14,"experiment":"wolf_diet","merged_sample":{"15a_F730814":11,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
aacttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:68:6976:3533#0/1_sub[27..126] {"ali_dir":"left","count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ttagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:107:7402:12373#0/1_sub[28..126] {"ali_dir":"left","count":11,"experiment":"wolf_diet","merged_sample":{"26a_F040644":11},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataaacattcaataaacaagaatgttcgctagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:35:16127:7152#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
aggttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:101:3925:10542#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(G:02)->(T:09)":61},"sample":"26a_F040644","score":192,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
tcctgaaactcaaaggactgggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:120:19194:8541#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":62,"pairing_fast_score":0.593,"pairing_mismatches":{"(A:07)->(T:02)":36,"(C:02)->(A:29)":69,"(C:16)->(T:02)":39,"(T:22)->(G:02)":22},"sample":"29a_F260619","score":149,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacacgtaattaatataacaaaataatccgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:41:7113:13306#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacgtaaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:119:14728:19318#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactgcgagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:33:8265:17718#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":63,"pairing_fast_score":0.683,"pairing_mismatches":{"(A:02)->(G:07)":51,"(A:30)->(G:07)":48,"(C:02)->(A:32)":58,"(C:02)->(G:10)":49,"(G:34)->(A:05)":32},"sample":"26a_F040644","score":1605,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtgctagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:49:12637:16989#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(a:02)->(c:32)":30,"(a:02)->(c:34)":70,"(a:02)->(t:34)":48,"(c:02)->(a:21)":42,"(g:02)->(c:30)":40},"sample":"29a_F260619","score":369,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaacagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:45:11254:12864#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{},"sample":"29a_F260619","score":3578,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagctctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:118:17090:10774#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:115:3353:16838#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(C:02)->(G:25)":56,"(G:02)->(C:29)":55},"sample":"29a_F260619","score":1506,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacccgcaat
agcttaaaactcaaaggacttggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:103:3749:19664#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:32)->(T:02)":34},"sample":"13a_F730603","score":912,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctaccggcaat
agcttaaaactcaaaggacttggcggtactttataccctt
>HELIUM_000100422_612GNAAXX:7:77:11866:13558#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(a:16)->(c:15)":42,"(a:34)->(g:02)":30},"sample":"26a_F040644","score":2828,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatattcgcaagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:1:4439:16308#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(a:32)->(c:32)":72,"(c:06)->(a:02)":42,"(g:02)->(t:34)":81,"(t:20)->(a:02)":35},"sample":"29a_F260619","score":877,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactmaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:76:8984:11823#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":28,"pairing_fast_overlap":62,"pairing_fast_score":0.475,"pairing_mismatches":{"(A:02)->(G:21)":52,"(A:31)->(C:02)":29,"(C:02)->(A:34)":67,"(C:02)->(G:11)":53,"(C:02)->(G:29)":56,"(C:02)->(T:31)":65,"(C:34)->(A:02)":30,"(T:22)->(G:02)":39},"sample":"29a_F260619","score":67,"score_norm":0.823,"seq_a_single":46,"seq_ab_match":51,"seq_b_single":46}
tcagccctaaacacaagtaattaatataacaaaattattcgccagagtgctggcggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:67:16428:2936#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"pairing_mismatches":{},"sample":"26a_F040644","score":3295,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaaggatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:120:3255:11738#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaacactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:65:19135:19068#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(c:02)->(t:35)":81,"(c:21)->(a:15)":58,"(c:26)->(a:33)":75,"(t:34)->(g:10)":39},"sample":"13a_F730603","score":2365,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggccat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:4:12329:3041#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":21,"experiment":"wolf_diet","merged_sample":{"15a_F730814":20,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactatcggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:115:3679:17919#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(G:02)->(T:09)":55,"(T:34)->(A:31)":21},"sample":"15a_F730814","score":1285,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaat
agcttaaaactcaaaggtcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:21:4904:4958#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":63,"pairing_fast_score":0.7,"pairing_mismatches":{"(a:02)->(c:34)":71,"(a:02)->(g:34)":76,"(a:09)->(c:32)":63,"(a:30)->(t:02)":33},"sample":"26a_F040644","score":1502,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataacaattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:40:14767:20097#0/1_sub[28..127] {"ali_dir":"left","count":11,"experiment":"wolf_diet","merged_sample":{"13a_F730603":11},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccagcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:61:13780:14696#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagaactactagcaac
agattaaacctcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:94:11433:12153#0/1_sub[28..127] {"ali_dir":"left","count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatacccta
>HELIUM_000100422_612GNAAXX:7:14:8480:20772#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttgtaccctt
>HELIUM_000100422_612GNAAXX:7:50:17151:20608#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:111:8748:5111#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(A:15)->(C:28)":72,"(T:10)->(A:33)":68,"(T:20)->(A:07)":35,"(T:30)->(G:17)":51},"sample":"29a_F260619","score":2596,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcgttgctttataccctt
>HELIUM_000100422_612GNAAXX:7:108:3401:14124#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctacacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:4:14278:6334#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(T:02)->(G:08)":39},"sample":"13a_F730603","score":970,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
caagccttaaacacaaatagttatgcaaacaaaactatgcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:96:13203:2879#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"13a_F730603":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaac
agcccaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:103:10067:19003#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"sample":"13a_F730603","score":1427,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaagtagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:25:7271:19962#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(c:06)->(t:09)":48,"(t:33)->(a:07)":35},"sample":"29a_F260619","score":3122,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaatttatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:77:3185:3942#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(c:20)->(a:34)":68,"(c:28)->(a:34)":74,"(c:31)->(a:31)":32},"sample":"13a_F730603","score":3343,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacamaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:20:17837:1390#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"26a_F040644","score":1564,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaata
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:14:5781:17228#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":26,"pairing_fast_overlap":62,"pairing_fast_score":0.441,"pairing_mismatches":{"(A:02)->(C:32)":43,"(A:02)->(G:15)":49,"(A:02)->(T:34)":36,"(A:02)->(T:35)":65,"(C:02)->(A:28)":69,"(C:02)->(G:07)":56,"(C:02)->(T:32)":39,"(T:02)->(C:26)":50},"sample":"29a_F260619","score":769,"score_norm":0.839,"seq_a_single":46,"seq_ab_match":52,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat
agcttaaaactcaagggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:35:10370:17180#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattaktcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:38:7815:6122#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(t:34)->(g:02)":38},"sample":"15a_F730814","score":2489,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacatgtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:78:18041:18996#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":13,"experiment":"wolf_diet","merged_sample":{"13a_F730603":9,"15a_F730814":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"seq_a_single":45,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:80:18387:10166#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaacgcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:111:17285:9660#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{},"sample":"29a_F260619","score":1652,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaagattattcgccagagaactactagcaac
agattaaacctcaaaggacttggcagtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:3:6465:20859#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{"(c:08)->(a:34)":73},"sample":"26a_F040644","score":2650,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccgt
>HELIUM_000100422_612GNAAXX:7:51:11736:18985#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(a:02)->(g:33)":77,"(a:02)->(t:10)":49,"(a:02)->(t:15)":69},"sample":"29a_F260619","score":965,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagttctaccggcaat
agcttaaatctcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:45:5221:16363#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":13,"experiment":"wolf_diet","merged_sample":{"15a_F730814":8,"29a_F260619":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaagcacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:10:15821:13492#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactaaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:64:17166:20424#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(A:02)->(G:09)":49,"(T:34)->(G:02)":21},"sample":"15a_F730814","score":747,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacgagtaattaatataacaaaattattcgccagagtgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttacaccctt
>HELIUM_000100422_612GNAAXX:7:27:6926:16557#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(A:15)->(G:02)":49,"(C:18)->(T:02)":39,"(C:22)->(T:17)":65,"(C:29)->(A:19)":67,"(T:22)->(A:02)":21},"sample":"29a_F260619","score":815,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacacgtaattaatataacaaaattatccgccagagtactaccggcaat
agctcacaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:89:6167:3127#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaactaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:109:1574:4169#0/1_sub[28..127] {"ali_dir":"left","count":8,"experiment":"wolf_diet","merged_sample":{"13a_F730603":8},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ctagccttaaacacaaatagttatgcaaacaaaactatacgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:15:14406:14205#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"15a_F730814","score":1763,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactctaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:98:18725:9928#0/1_sub[28..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":64,"pairing_fast_score":0.934,"pairing_mismatches":{"(C:10)->(A:33)":67},"sample":"26a_F040644","score":4026,"score_norm":0.984,"seq_a_single":44,"seq_ab_match":63,"seq_b_single":44}
ttagcctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacag
cctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:61:6786:9191#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"29a_F260619","score":2020,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaacttaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:110:8881:8672#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(A:02)->(T:09)":54,"(T:02)->(G:13)":53},"sample":"26a_F040644","score":1352,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacgtgcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:18:17533:5304#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":62,"pairing_fast_score":0.949,"pairing_mismatches":{},"sample":"15a_F730814","score":3481,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaatgaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:39:11059:12749#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":63,"pairing_fast_score":0.817,"pairing_mismatches":{"(c:20)->(a:31)":68,"(c:21)->(t:02)":38,"(c:26)->(a:02)":41},"sample":"26a_F040644","score":1039,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaatataaacattcaataaacaagaatgtccgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:4:9303:16896#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":63,"pairing_fast_score":0.983,"pairing_mismatches":{},"sample":"26a_F040644","score":2232,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagagtactactagcaaca
gcctaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:63:17517:2795#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"sample":"29a_F260619","score":2091,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatccgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:54:10113:12172#0/1_sub[28..127] {"ali_dir":"left","count":7,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:53:2796:5395#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":7,"experiment":"wolf_diet","merged_sample":{"26a_F040644":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccttaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:116:15644:10347#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"pairing_mismatches":{},"sample":"29a_F260619","score":3483,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaacacaagtaattaatataacaaaattattcgccagagtactaccggcaata
gtttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:113:7291:4066#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagctctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:105:19634:10078#0/1_sub[28..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":64,"pairing_fast_score":0.656,"pairing_mismatches":{"(a:02)->(g:34)":75,"(a:32)->(t:02)":34,"(c:02)->(g:34)":81,"(c:08)->(a:31)":40,"(c:10)->(t:34)":63,"(c:25)->(a:02)":32,"(t:27)->(g:02)":36},"sample":"26a_F040644","score":1868,"score_norm":0.891,"seq_a_single":44,"seq_ab_match":57,"seq_b_single":44}
ttagccctaaacataaacattctataacaaactaattcgacagagaactactagcaacag
cttaaaactcaaaggacttggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:115:11627:7430#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:02)->(C:30)":54},"sample":"13a_F730603","score":784,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaatagttatgcatacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:39:10651:3891#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":8,"experiment":"wolf_diet","merged_sample":{"15a_F730814":6,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttacaccctt
>HELIUM_000100422_612GNAAXX:7:117:13156:8661#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":1794,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaac
agcccaaaactcaaaggacttggtggtgcttcacaccctt
>HELIUM_000100422_612GNAAXX:7:88:11489:4463#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(G:27)->(A:13)":33},"sample":"29a_F260619","score":2512,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaagattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:7:10951:7589#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(a:07)->(c:02)":50,"(c:05)->(a:02)":49,"(t:09)->(a:02)":44,"(t:33)->(a:02)":35},"sample":"15a_F730814","score":1648,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgcctgagtcataccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:101:17340:4476#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaacttaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:112:7264:14296#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(c:16)->(t:33)":65,"(c:22)->(a:22)":74},"sample":"13a_F730603","score":560,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcamaggacttggcggtgcgttataccctt
>HELIUM_000100422_612GNAAXX:7:29:19216:14639#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":63,"pairing_fast_score":0.717,"pairing_mismatches":{"(A:32)->(G:15)":51,"(C:02)->(A:32)":58,"(G:02)->(A:10)":55,"(T:30)->(G:13)":38},"sample":"26a_F040644","score":1071,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactaacaaca
gcctgaaactcaaaggacttgggggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:41:13107:8094#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
tcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:14:13051:2456#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(c:06)->(g:34)":56,"(c:34)->(a:02)":42,"(t:33)->(g:02)":35},"sample":"29a_F260619","score":1453,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:93:1967:6635#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(c:09)->(t:23)":62,"(t:33)->(c:02)":37},"sample":"26a_F040644","score":2115,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gtctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:5:18747:12870#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(a:06)->(c:02)":43,"(c:02)->(a:15)":75,"(t:02)->(g:08)":80},"sample":"13a_F730603","score":261,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgcaagagtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:85:9809:16636#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(c:11)->(t:02)":47},"sample":"26a_F040644","score":1706,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagcactactagcaaca
gcctgaaactcaaaggacgtggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:62:4250:9317#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(C:06)":49,"(A:02)->(G:07)":58,"(G:26)->(C:02)":41},"sample":"13a_F730603","score":187,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtcctaccggcgat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:10:6099:10165#0/1_sub[27..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":64,"pairing_fast_score":0.738,"pairing_mismatches":{"(A:02)->(T:07)":57,"(G:02)->(T:07)":55},"sample":"26a_F040644","score":1690,"score_norm":0.891,"seq_a_single":44,"seq_ab_match":57,"seq_b_single":44}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactatctaca
gcctgaaactcaaagggcttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:113:17232:21012#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtatttaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:99:9430:20842#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":17,"experiment":"wolf_diet","merged_sample":{"15a_F730814":14,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattagtataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:11:15998:7539#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":12,"experiment":"wolf_diet","merged_sample":{"26a_F040644":12},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:71:4701:18905#0/1_sub[28..127] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(a:25)->(t:02)":49},"sample":"29a_F260619","score":28,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacacaagaaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:63:13445:5120#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":11,"experiment":"wolf_diet","merged_sample":{"26a_F040644":11},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactaacaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:80:13210:20670#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":9,"experiment":"wolf_diet","merged_sample":{"15a_F730814":8,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttaaccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:19:15763:17447#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(A:02)->(G:07)":52,"(C:02)->(G:15)":56,"(C:02)->(T:34)":65,"(G:02)->(A:10)":55,"(T:34)->(A:15)":27},"sample":"15a_F730814","score":263,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccagcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:50:5388:9307#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":63,"pairing_fast_score":0.667,"pairing_mismatches":{"(a:29)->(c:29)":63,"(a:34)->(g:02)":33,"(c:02)->(t:34)":80,"(t:22)->(g:02)":37},"sample":"26a_F040644","score":1738,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcmtgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:3:6620:11576#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacagtcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:27:5515:20557#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(a:13)->(t:02)":47,"(a:14)->(c:02)":42},"sample":"26a_F040644","score":692,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgcaagagaactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:97:17822:18365#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:73:10569:21225#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaaggttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:76:7624:17225#0/1_sub[78..81] {"ali_dir":"right","ali_length":58,"count":3,"experiment":"wolf_diet","merged_sample":{"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":58,"pairing_mismatches":{},"sample":"29a_F260619","seq_a_single":50,"seq_b_single":50}
ttct
>HELIUM_000100422_612GNAAXX:7:86:13552:17152#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":1,"obimultiplex_reverse_match":"tagaacaggctcctctcg","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":63,"pairing_fast_score":0.717,"pairing_mismatches":{"(c:02)->(a:34)":58,"(c:13)->(a:02)":48,"(t:26)->(g:02)":34},"sample":"26a_F040644","score":220,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggcctactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:51:8186:6303#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(a:02)->(t:26)":48,"(c:02)->(a:34)":75,"(c:02)->(g:28)":47,"(c:02)->(g:32)":53,"(c:02)->(g:33)":45,"(t:02)->(g:29)":56},"sample":"29a_F260619","score":1106,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaactaattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:92:3562:12237#0/1_sub[27..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(c:22)->(t:34)":81},"sample":"13a_F730603","score":3741,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttcgcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:33:15193:4157#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(a:24)->(c:24)":43},"sample":"15a_F730814","score":2881,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgcmagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctc
>HELIUM_000100422_612GNAAXX:7:108:14522:1643#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":63,"pairing_fast_score":0.733,"pairing_mismatches":{"(A:02)->(C:18)":57,"(C:02)->(A:34)":58,"(G:02)->(C:34)":63,"(T:34)->(A:02)":21},"sample":"26a_F040644","score":419,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagccaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:39:16341:5957#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(A:02)->(T:10)":58,"(C:33)->(A:02)":30,"(G:02)->(T:09)":56,"(T:30)->(G:02)":27},"sample":"29a_F260619","score":913,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
tcagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccgtctat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:15:3673:6055#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(A:02)->(G:08)":52},"sample":"15a_F730814","score":1092,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcgat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:22:1548:20854#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(C:02)->(G:10)":53,"(T:31)->(A:09)":24},"sample":"13a_F730603","score":1255,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactagcggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:23:13701:17041#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":7,"experiment":"wolf_diet","merged_sample":{"26a_F040644":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcgataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:92:16215:17298#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":17,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4,"29a_F260619":13},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgacagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:98:4349:2021#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:41:11214:2738#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":17,"experiment":"wolf_diet","merged_sample":{"13a_F730603":17},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgtcagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:120:4975:15399#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(A:02)->(G:15)":51,"(C:02)->(G:28)":62},"sample":"26a_F040644","score":1831,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactgctagcaaca
ggctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:16:7840:15604#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaagactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:32:1980:4009#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagaggactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:18:9748:19834#0/1_sub[28..127] {"ali_dir":"left","count":6,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0}
ttagccctaaacacaagtaattaatataacaaaattattcgctagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:111:17532:1250#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(C:34)":54,"(C:02)->(A:31)":59,"(C:02)->(T:34)":65},"sample":"13a_F730603","score":737,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggttgtttataccctt
>HELIUM_000100422_612GNAAXX:7:73:16096:5953#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3219,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacatgaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctctacatccct
>HELIUM_000100422_612GNAAXX:7:93:5576:4583#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":63,"pairing_fast_score":0.65,"pairing_mismatches":{"(A:02)->(C:30)":42,"(C:02)->(A:17)":58,"(G:02)->(T:09)":61,"(T:02)->(C:34)":63,"(T:02)->(G:21)":38},"sample":"26a_F040644","score":763,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtgcgccagagtactactagcaaca
tcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:105:17460:15581#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3560,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactaccagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:104:12948:6375#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttttaccctt
>HELIUM_000100422_612GNAAXX:7:118:6838:2776#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(C:02)->(G:34)":56,"(T:34)->(G:06)":21},"sample":"13a_F730603","score":1628,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaaataattatataaacaaaattattcgccagagtactaccggcaac
agctcaaaactcaaaggacttggcggtgcttcacaccctt
>HELIUM_000100422_612GNAAXX:7:95:18422:18757#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:18:10702:9498#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":2543,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaatg
gcctaaaactcaaaggacttggtggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:55:9949:20170#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
atcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:5:16299:13461#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(a:02)->(g:18)":78,"(a:07)->(t:02)":48,"(c:02)->(t:32)":81,"(c:23)->(g:02)":42,"(c:30)->(a:02)":53,"(t:24)->(a:02)":35,"(t:28)->(a:02)":38},"sample":"15a_F730814","score":150,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagaactaccggcaat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:70:5335:3060#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(C:02)->(A:26)":59,"(T:26)->(A:02)":24},"sample":"13a_F730603","score":1138,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgcttgatcccctt
>HELIUM_000100422_612GNAAXX:7:107:18026:11098#0/1_sub[27..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(a:02)->(g:31)":77,"(a:15)->(t:15)":48},"sample":"29a_F260619","score":1795,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
tcagccctaaacacaagtaattaatataacaaaattattcgccagagwactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:120:6249:11331#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(C:02)->(G:12)":50,"(C:27)->(G:02)":26},"sample":"13a_F730603","score":1113,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtagtaccggcaat
agcttaatactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:58:19025:10053#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(a:02)->(c:27)":43,"(t:02)->(a:10)":38},"sample":"15a_F730814","score":579,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaaataatataacaaaattaatcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:39:19044:19392#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":63,"pairing_fast_score":0.583,"pairing_mismatches":{"(A:30)->(G:02)":48,"(A:32)->(G:02)":31,"(C:32)->(G:02)":42,"(C:33)->(A:02)":22,"(G:32)->(T:09)":65,"(T:32)->(G:06)":64},"sample":"26a_F040644","score":1211,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcagtgctttatacctct
>HELIUM_000100422_612GNAAXX:7:87:6328:12406#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":6,"experiment":"wolf_diet","merged_sample":{"26a_F040644":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:44:11757:20006#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(C:02)->(A:34)":59},"sample":"13a_F730603","score":1856,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaagcacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
aacttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:113:19475:7252#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(a:34)->(g:02)":31,"(c:02)->(a:34)":74,"(c:14)->(a:02)":34,"(c:28)->(a:34)":68,"(g:02)->(t:34)":81,"(t:02)->(g:26)":80,"(t:29)->(g:02)":38},"sample":"13a_F730603","score":1016,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaacctattcgccagagtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:62:16550:12892#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":21,"experiment":"wolf_diet","merged_sample":{"26a_F040644":21},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggtggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:82:14722:2961#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"13a_F730603":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaacgcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:4611:13145#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtacgtccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:94:5109:10098#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":11,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3,"29a_F260619":8},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactactggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:1:10863:9819#0/1_sub[28..127] {"ali_dir":"left","count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ctagccttaaacacaaatagktatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:3:4930:6412#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(g:02)->(c:33)":79,"(t:09)->(a:02)":59},"sample":"15a_F730814","score":629,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcatt
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:33:5763:11589#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(T:02)->(C:08)":38,"(T:02)->(G:08)":48},"sample":"15a_F730814","score":1077,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattactcgccagaggactaccggcaat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:115:5193:11386#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacttgcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:3:19417:19441#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(A:02)->(G:10)":67,"(A:02)->(T:14)":65,"(C:02)->(A:14)":59,"(T:02)->(G:09)":48,"(T:33)->(C:06)":38,"(T:34)->(A:21)":21},"sample":"15a_F730814","score":1331,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagaggactaccggcaat
agcttagaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:89:10093:21198#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(C:02)->(A:34)":59,"(C:17)->(T:02)":36,"(C:32)->(G:02)":42,"(G:32)->(T:02)":41,"(T:02)->(G:09)":71},"sample":"29a_F260619","score":784,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaatcattcgccagagtactaccggcaat
agcttaaaacgcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:88:2542:2726#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggtcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:45:11239:15851#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"26a_F040644","score":1912,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaaccattctataacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:71:7003:2425#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":27,"experiment":"wolf_diet","merged_sample":{"15a_F730814":13,"29a_F260619":14},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaagggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:102:7548:5526#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaacctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:88:5230:16215#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":3855,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacataaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:78:8867:3762#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(c:02)->(a:34)":74,"(c:19)->(a:34)":67},"sample":"29a_F260619","score":2232,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagaaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:97:17532:15049#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"sample":"15a_F730814","score":1251,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcgtggctttataccctt
>HELIUM_000100422_612GNAAXX:7:53:9807:10455#0/1_sub[28..127] {"ali_dir":"left","count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603","seq_ab_match":62}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcagtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:78:9269:16255#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaacttattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:77:5256:9828#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgcttcacatccct
>HELIUM_000100422_612GNAAXX:7:104:18765:4925#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(c:02)->(a:21)":69,"(c:02)->(t:34)":81,"(t:02)->(g:10)":64},"sample":"29a_F260619","score":566,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcgtaaaactcaaaggacttggcggcgctttataccctt
>HELIUM_000100422_612GNAAXX:7:92:18634:15687#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":29,"pairing_fast_overlap":62,"pairing_fast_score":0.492,"pairing_mismatches":{"(a:34)->(g:02)":29,"(a:34)->(t:07)":58,"(c:02)->(a:22)":59,"(c:02)->(a:24)":75,"(c:02)->(t:32)":81,"(c:34)->(g:02)":42,"(t:02)->(g:20)":80,"(t:29)->(g:02)":35},"sample":"29a_F260619","score":414,"score_norm":0.823,"seq_a_single":46,"seq_ab_match":51,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttatacccgt
>HELIUM_000100422_612GNAAXX:7:90:7340:4675#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(c:13)->(g:02)":44},"sample":"26a_F040644","score":1550,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccacaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:56:9848:6178#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":63,"pairing_fast_score":0.667,"pairing_mismatches":{"(A:21)->(C:02)":34,"(C:07)->(A:02)":58,"(C:09)->(T:02)":38,"(C:34)->(A:02)":22,"(T:09)->(C:02)":37,"(T:23)->(G:02)":53},"sample":"26a_F040644","score":192,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtccgccagagtactactagcacca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:115:18867:6048#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(c:30)->(a:15)":42},"sample":"13a_F730603","score":1663,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttgcataaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:104:13489:17538#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(C:02)->(A:29)":59,"(C:02)->(T:34)":65,"(G:02)->(T:24)":56,"(T:02)->(G:22)":39,"(T:34)->(G:02)":21},"sample":"15a_F730814","score":736,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatgcgccagagtactaccgtcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:114:11301:15600#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(c:02)->(t:34)":81,"(c:07)->(g:02)":45},"sample":"13a_F730603","score":269,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccacagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:109:3431:18588#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":63,"pairing_fast_score":0.583,"pairing_mismatches":{"(a:31)->(g:02)":34,"(a:34)->(g:02)":30,"(c:23)->(t:14)":80,"(c:31)->(g:02)":41,"(t:15)->(c:02)":63,"(t:31)->(g:02)":37},"sample":"26a_F040644","score":267,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcttgaaactcaaaggactcggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:54:17007:10875#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":62,"pairing_fast_score":0.949,"pairing_mismatches":{"(T:34)->(G:02)":21},"sample":"15a_F730814","score":891,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagggtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:110:17951:16037#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:02)->(C:34)":54,"(C:02)->(G:15)":42,"(C:28)->(G:06)":26,"(G:02)->(A:07)":41},"sample":"13a_F730603","score":645,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcagcagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:51:16702:19393#0/1_sub[28..127] {"ali_dir":"left","count":12004,"experiment":"wolf_diet","merged_sample":{"15a_F730814":7465,"29a_F260619":4539},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(c:27)->(a:25)":67,"(c:28)->(a:27)":59,"(t:28)->(g:23)":38,"(t:29)->(g:16)":80},"sample":"13a_F730603","score":2491,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcact
agcttacaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:14:6042:19322#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(A:02)->(C:23)":54,"(A:02)->(G:06)":52,"(C:02)->(A:34)":68,"(G:02)->(T:16)":62,"(T:25)->(A:02)":21},"sample":"29a_F260619","score":612,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat
atcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:78:6346:5817#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"13a_F730603":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaaataattatataaacaaaattattcgccagagtactaccggcaac
agcccaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:15122:17310#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtacttcctgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:115:19404:7379#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":63,"pairing_fast_score":0.567,"pairing_mismatches":{"(a:33)->(g:02)":30,"(c:02)->(a:30)":73,"(c:02)->(t:32)":80,"(c:06)->(t:02)":47,"(t:02)->(g:07)":79,"(t:28)->(c:02)":37},"sample":"26a_F040644","score":862,"score_norm":0.857,"seq_a_single":45,"seq_ab_match":54,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagcactactagcaaca
gcctgaaactcaaaggacgtggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:27:7695:17738#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":7,"experiment":"wolf_diet","merged_sample":{"26a_F040644":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:116:2647:5120#0/1_sub[28..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":64,"pairing_fast_score":0.557,"pairing_mismatches":{"(A:19)->(C:09)":58,"(A:27)->(G:02)":48,"(A:34)->(G:02)":31,"(G:32)->(T:02)":36,"(T:02)->(C:06)":70,"(T:32)->(G:02)":35},"sample":"26a_F040644","score":182,"score_norm":0.859,"seq_a_single":44,"seq_ab_match":55,"seq_b_single":44}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaacccaaaggcctgggcggtgcttacatccct
>HELIUM_000100422_612GNAAXX:7:9:2930:13877#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(A:14)->(N:02)":28,"(G:02)->(A:02)":46},"sample":"26a_F040644","score":5,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagargactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:97:2916:4790#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{"(c:02)->(a:32)":77},"sample":"26a_F040644","score":2974,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagctctaaacataaacattcaataaacaagaatgttcgccagagtactactagcatca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:113:5095:16800#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:02)->(G:25)":34,"(G:02)->(T:10)":56},"sample":"13a_F730603","score":712,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaagctattcgccagagtactaccgtcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:24:18870:19644#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(A:02)->(C:08)":52,"(C:02)->(A:29)":59,"(C:34)->(A:02)":30,"(G:06)->(T:02)":35,"(T:34)->(A:02)":21,"(T:34)->(G:02)":27},"sample":"15a_F730814","score":498,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtattcgccagagtactcccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:35:1680:19224#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(a:11)->(t:23)":71,"(a:25)->(g:02)":33,"(t:16)->(g:02)":35,"(t:28)->(c:02)":38},"sample":"29a_F260619","score":2210,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacctggcggtgcttcatatccct
>HELIUM_000100422_612GNAAXX:7:24:5186:1448#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:31)->(T:02)":23,"(C:31)->(A:02)":32},"sample":"29a_F260619","score":430,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacacaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:56:19358:6898#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(G:34)->(A:02)":32},"sample":"26a_F040644","score":1475,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatatacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:34:14477:20365#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(T:02)->(G:10)":50},"sample":"26a_F040644","score":1682,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaatcattctataacaaaataattcgccggagaacgactaggaaca
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:47:1914:4052#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3516,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagagaactactagcaaca
gcttaaaactcaaaggacttggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:64:19363:5772#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":23,"pairing_fast_overlap":63,"pairing_fast_score":0.383,"pairing_mismatches":{"(a:22)->(g:02)":51,"(a:23)->(g:07)":73,"(c:07)->(a:24)":68,"(c:15)->(a:32)":66,"(c:23)->(a:02)":33,"(c:31)->(a:02)":41,"(t:27)->(g:02)":37,"(t:28)->(g:19)":79},"sample":"26a_F040644","score":621,"score_norm":0.81,"seq_a_single":45,"seq_ab_match":51,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagcatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:112:7859:1190#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"sample":"29a_F260619","score":1671,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctagacacaagtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:29:9075:19187#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":63,"pairing_fast_score":0.667,"pairing_mismatches":{"(A:13)->(G:06)":58,"(A:32)->(G:02)":26,"(A:34)->(G:02)":31,"(C:28)->(G:28)":62,"(T:33)->(G:10)":38},"sample":"26a_F040644","score":1745,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gsctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:22:5058:9164#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(C:02)->(A:25)":59,"(C:13)->(A:02)":32},"sample":"29a_F260619","score":321,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacacaattattcgccagagtactaccggtaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:18:15367:17940#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(A:02)->(C:34)":63},"sample":"15a_F730814","score":1324,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctcaacacacgtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:1:13467:8688#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":63,"pairing_fast_score":0.7,"pairing_mismatches":{"(a:15)->(c:02)":42,"(a:28)->(g:02)":30,"(a:32)->(g:02)":33,"(t:06)->(a:02)":58},"sample":"26a_F040644","score":1131,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcatca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:23:1418:7833#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":63,"pairing_fast_score":0.733,"pairing_mismatches":{"(C:02)->(A:27)":58,"(T:02)->(G:15)":53},"sample":"29a_F260619","score":503,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactacgagcaaca
gattaaacctcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:12:17700:2681#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaacccaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:87:18462:8148#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(T:34)->(G:02)":20,"(T:34)->(G:06)":35},"sample":"26a_F040644","score":2460,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgctactagcaaca
gcctgaaactcaaaggacttggcggtgctttacgtccct
>HELIUM_000100422_612GNAAXX:7:21:6756:20138#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":15,"experiment":"wolf_diet","merged_sample":{"26a_F040644":15},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataagcaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:51:17768:19838#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtggtttataccctt
>HELIUM_000100422_612GNAAXX:7:98:2721:11411#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":11,"experiment":"wolf_diet","merged_sample":{"15a_F730814":7,"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctagacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:117:15910:10176#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(C:32)->(A:02)":22,"(T:34)->(G:02)":20},"sample":"26a_F040644","score":1331,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggactaggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:82:11206:1620#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":63,"pairing_fast_score":0.633,"pairing_mismatches":{"(C:02)->(A:14)":58,"(C:02)->(A:32)":68,"(T:33)->(G:02)":25,"(T:34)->(G:02)":21},"sample":"26a_F040644","score":635,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gccagaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:93:8074:11114#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagctctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:59:1822:11863#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"26a_F040644","score":1706,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gccggaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:97:14740:3107#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(A:02)->(G:08)":69,"(A:02)->(T:07)":49,"(T:02)->(G:10)":71},"sample":"15a_F730814","score":901,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagttctaccggcaat
agcttaaagcgcagaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:5:1466:9914#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(a:15)->(t:34)":81,"(a:33)->(g:20)":33,"(c:10)->(g:34)":77,"(c:21)->(a:07)":69,"(c:22)->(a:34)":75,"(t:29)->(g:10)":35},"sample":"29a_F260619","score":2678,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgctagagtactaccggcaat
agcttaaacctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:114:5854:17578#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(a:16)->(c:02)":43,"(t:33)->(a:02)":35},"sample":"15a_F730814","score":535,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgcaagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:98:19275:16178#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":31,"pairing_fast_overlap":63,"pairing_fast_score":0.517,"pairing_mismatches":{"(a:28)->(g:02)":33,"(c:02)->(a:34)":74,"(c:02)->(g:34)":76,"(c:18)->(a:02)":34,"(c:22)->(a:15)":66,"(c:28)->(t:09)":62,"(c:29)->(a:02)":41,"(t:26)->(g:02)":38},"sample":"26a_F040644","score":729,"score_norm":0.857,"seq_a_single":45,"seq_ab_match":54,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagactgttcgccagagtactactagcaaca
gcctgcaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:74:19706:7811#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":30,"pairing_fast_overlap":63,"pairing_fast_score":0.5,"pairing_mismatches":{"(a:07)->(c:02)":42,"(c:02)->(a:25)":68,"(c:02)->(g:34)":76,"(c:02)->(t:32)":80,"(c:22)->(t:02)":38,"(g:02)->(c:28)":69,"(g:02)->(c:32)":63,"(t:02)->(g:09)":70},"sample":"26a_F040644","score":165,"score_norm":0.81,"seq_a_single":45,"seq_ab_match":51,"seq_b_single":45}
ttagccctaaacataaacattaaataaacaagaatgtccgcaagagtactactagcaaca
gcctgaaacgcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:23:15940:15154#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":63,"pairing_fast_score":0.717,"pairing_mismatches":{"(a:34)->(g:02)":33,"(c:25)->(a:02)":58,"(c:34)->(a:02)":41,"(t:02)->(g:09)":79},"sample":"26a_F040644","score":479,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcacca
gcctgaaactcaaaggacgtggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:76:18066:19593#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaacggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:86:8429:13723#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"15a_F730814":5,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggactcggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:42:18875:8727#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(C:10)->(A:28)":68,"(T:20)->(G:02)":35,"(T:34)->(A:02)":21},"sample":"29a_F260619","score":599,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttacccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:76:7055:12333#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{},"sample":"26a_F040644","score":2638,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccgtaaacataaatcattctacaacaaaataattcgccagagaactattagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:100:17918:10327#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"13a_F730603":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactagtcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:48:15379:13773#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacatagataattttacaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:7:1735:19736#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3,"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctg
>HELIUM_000100422_612GNAAXX:7:69:15411:19751#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(c:02)->(t:32)":81,"(c:26)->(g:02)":42,"(t:18)->(g:02)":39},"sample":"13a_F730603","score":467,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttagaccctt
>HELIUM_000100422_612GNAAXX:7:118:2634:4776#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":63,"pairing_fast_score":0.6,"pairing_mismatches":{"(A:24)->(C:02)":30,"(C:02)->(A:23)":58,"(C:06)->(A:02)":24,"(G:28)->(T:19)":36,"(T:25)->(G:31)":37,"(T:26)->(G:18)":35},"sample":"26a_F040644","score":634,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacataaacattcactaaacaaaaatggtcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:10:18771:20660#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactacctgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:23:12040:7242#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":8,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4,"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccttaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:73:12897:11007#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(a:02)->(g:33)":76},"sample":"26a_F040644","score":2553,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcatca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:117:16246:17184#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"13a_F730603":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagaggactactagcaat
agcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:108:15741:20168#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:02)->(C:24)":54,"(G:02)->(T:13)":55},"sample":"29a_F260619","score":1035,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:55:15924:4682#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(A:10)->(C:02)":42,"(T:34)->(A:02)":20},"sample":"26a_F040644","score":336,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaaca
gcctgaaactcaaaggacgtggcggtgctttacatcccg
>HELIUM_000100422_612GNAAXX:7:9:1900:13770#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":63,"pairing_fast_score":0.733,"pairing_mismatches":{"(A:32)->(N:02)":28,"(C:02)->(A:30)":67,"(T:02)->(G:16)":37,"(T:34)->(G:12)":35},"sample":"26a_F040644","score":189,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatggtcgcccgagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:70:11590:7666#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(C:02)->(G:02)":49,"(G:29)->(A:02)":32,"(T:32)->(G:02)":38},"sample":"26a_F040644","score":7,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtastactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:95:18573:13686#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgctgctagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:87:11044:6323#0/1_sub[28..126] {"ali_dir":"left","count":69,"experiment":"wolf_diet","merged_sample":{"26a_F040644":69},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:35:12606:18312#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":20,"experiment":"wolf_diet","merged_sample":{"15a_F730814":12,"29a_F260619":8},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatccgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:17360:10821#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggtaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:40:12248:18615#0/1_sub[28..126] {"ali_dir":"left","count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataagctattctataacaaaataattcgccagagaactactagcaaca
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:7:6470:13562#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtacctccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:119:3969:16506#0/1_sub[28..127] {"ali_dir":"left","count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:95:3398:2166#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcgtaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:18:18091:9066#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(A:02)->(C:31)":72,"(A:02)->(G:08)":52,"(C:02)->(A:24)":69,"(C:02)->(A:33)":67,"(C:02)->(G:06)":56,"(C:02)->(T:31)":65,"(T:02)->(G:10)":64},"sample":"15a_F730814","score":45,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat
agcgtaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:70:19318:3248#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(a:27)->(g:02)":34,"(c:02)->(a:32)":68,"(c:02)->(t:30)":65,"(c:22)->(a:02)":33,"(t:02)->(g:18)":71,"(t:02)->(g:34)":77,"(t:34)->(g:02)":39},"sample":"29a_F260619","score":162,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaccacaagtaattaatataacaacattattcgccagagtactaccggcaat
agcttaaaacgcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:117:16880:16992#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:02)->(C:14)":54,"(A:02)->(G:15)":58,"(T:02)->(G:16)":38},"sample":"15a_F730814","score":862,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattagtcgccagagtactaccggcgat
agcttaaaactcaaagtacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:75:3736:4689#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:28)->(G:02)":31,"(C:31)->(A:02)":30,"(T:20)->(G:22)":36},"sample":"29a_F260619","score":597,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaatgattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:93:1751:13433#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(A:34)->(G:02)":33,"(C:09)->(T:34)":65,"(C:19)->(A:02)":32,"(T:17)->(G:02)":39},"sample":"13a_F730603","score":1359,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacacaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:54:16515:19743#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(a:02)->(c:34)":79,"(a:34)->(g:02)":33,"(c:02)->(a:32)":68,"(c:02)->(a:34)":75,"(c:02)->(g:18)":80,"(c:13)->(a:26)":52,"(t:02)->(g:09)":71},"sample":"29a_F260619","score":857,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaacgcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:70:4473:11827#0/1_sub[27..126] {"ali_dir":"left","count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtaccaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:54:17543:21186#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(a:02)->(g:34)":77,"(a:09)->(c:02)":40,"(c:02)->(a:30)":61,"(c:02)->(a:34)":67,"(t:02)->(a:34)":74,"(t:22)->(c:02)":38},"sample":"29a_F260619","score":315,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattagccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:67:11420:4663#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3452,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccaggggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:24:17247:14366#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaagggcttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:78:15166:19673#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(c:02)->(a:34)":75,"(c:02)->(g:34)":77,"(c:02)->(t:34)":81,"(c:14)->(t:02)":39,"(c:23)->(g:26)":56,"(t:02)->(g:20)":64},"sample":"13a_F730603","score":577,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactatccgccagagtactaccggcaat
agcgtaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:68:3713:9432#0/1_sub[28..127] {"ali_dir":"left","count":11,"experiment":"wolf_diet","merged_sample":{"15a_F730814":5,"29a_F260619":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggcgctttataccctt
>HELIUM_000100422_612GNAAXX:7:72:11850:15705#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:91:19258:12097#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(A:02)->(C:15)":49,"(C:02)->(A:17)":61,"(C:02)->(G:07)":42,"(C:02)->(G:08)":69,"(T:02)->(G:28)":35},"sample":"29a_F260619","score":326,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtattcggcagagtcctaccggcaat
agcttaaagctcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:91:5667:20623#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(c:02)->(g:34)":77,"(c:17)->(a:02)":44,"(t:20)->(a:02)":35},"sample":"29a_F260619","score":131,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgcccgagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:33:19700:1110#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":30,"pairing_fast_overlap":63,"pairing_fast_score":0.5,"pairing_mismatches":{"(a:31)->(t:02)":33,"(c:02)->(a:25)":68,"(c:07)->(g:02)":46,"(c:15)->(a:02)":41,"(t:02)->(g:13)":70,"(t:02)->(g:31)":75,"(t:29)->(g:02)":53},"sample":"26a_F040644","score":458,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacatcaacattcactaaacaagaatgttcgccagactactactagcaaca
gcctgaaacgcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:82:8566:4827#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":6,"experiment":"wolf_diet","merged_sample":{"26a_F040644":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaaggatgttcgcaagagtactactagcaatg
gcctaaaactcaaaggacttggtggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:21:8628:1493#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactactagcaaca
gattaaacctcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:48:3126:7436#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:02)->(G:09)":52,"(A:12)->(T:02)":32},"sample":"13a_F730603","score":623,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:108:5996:3220#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":3311,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaataattatataaacaaaattattcgccagagtactaccggcaac
agcccaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:82:14102:9440#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(a:02)->(c:31)":63,"(a:02)->(c:32)":54,"(a:02)->(g:34)":77,"(a:02)->(t:28)":48,"(c:02)->(a:06)":42},"sample":"29a_F260619","score":365,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagaaattaatataacaaaattattcgacagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:116:3794:7045#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(A:28)->(G:06)":52,"(C:02)->(T:34)":65,"(C:23)->(G:24)":42,"(T:11)->(G:07)":35,"(T:34)->(A:02)":21},"sample":"29a_F260619","score":1078,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcggcagagtactaccggcaat
agcttaaaactcaaagggcgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:28:5713:13195#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
tcagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:95:4492:1521#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":63,"pairing_fast_score":0.883,"pairing_mismatches":{"(c:06)->(g:34)":76,"(c:24)->(a:34)":74},"sample":"13a_F730603","score":2353,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctctatatccct
>HELIUM_000100422_612GNAAXX:7:38:7385:19847#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagacttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:87:11368:15924#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"29a_F260619","score":1639,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:104:3963:1962#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:02)->(G:06)":52,"(C:02)->(A:33)":59,"(C:02)->(T:34)":65},"sample":"29a_F260619","score":1163,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:73:11173:18517#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(A:34)->(G:09)":30},"sample":"26a_F040644","score":1269,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcataggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:98:13831:3688#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(a:20)->(t:22)":58,"(c:17)->(a:34)":74},"sample":"29a_F260619","score":3044,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattattcgccagagtactaccggctat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:26:14745:19127#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":62,"pairing_fast_score":0.983,"pairing_mismatches":{"(c:02)->(t:34)":81},"sample":"29a_F260619","score":3490,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaataattcgccagagaactactagcaac
agattaaacctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:78:6876:12995#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(A:02)->(C:10)":52,"(C:02)->(A:23)":59,"(C:02)->(G:23)":56},"sample":"29a_F260619","score":196,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactcccggcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:115:5817:2006#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":18,"experiment":"wolf_diet","merged_sample":{"15a_F730814":11,"29a_F260619":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccagcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:72:19103:2288#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":31,"pairing_fast_overlap":62,"pairing_fast_score":0.525,"pairing_mismatches":{"(a:02)->(g:32)":77,"(a:15)->(t:02)":48,"(c:11)->(a:02)":59,"(c:34)->(g:02)":42,"(t:02)->(g:16)":71,"(t:30)->(g:02)":38},"sample":"29a_F260619","score":337,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagaactaccggcact
agcttaaaacgcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:55:10755:16757#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctcaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:65:7728:8793#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(C:02)->(A:34)":59},"sample":"29a_F260619","score":1751,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaacctcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:35:4900:9406#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":63,"pairing_fast_score":0.7,"pairing_mismatches":{"(a:02)->(c:27)":69,"(a:02)->(c:29)":63,"(a:02)->(g:10)":48,"(a:28)->(c:02)":33},"sample":"26a_F040644","score":348,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaaaaatgttcgccagagtgctactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:78:15647:12722#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":8,"experiment":"wolf_diet","merged_sample":{"15a_F730814":7,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttggccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:6:7893:17320#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcctaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:17:6908:13134#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":63,"pairing_fast_score":0.983,"pairing_mismatches":{},"sample":"26a_F040644","score":2034,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatattcct
>HELIUM_000100422_612GNAAXX:7:100:14658:18499#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":32,"pairing_fast_overlap":62,"pairing_fast_score":0.542,"pairing_mismatches":{"(A:24)->(G:16)":34,"(A:30)->(G:16)":37,"(C:02)->(A:33)":59,"(C:02)->(G:32)":56,"(C:02)->(T:27)":65,"(T:07)->(G:20)":39,"(T:33)->(G:11)":21},"sample":"15a_F730814","score":1200,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatgcgccagagtactaccggcaat
agcttaaaactcaaaggatttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:47:3759:8030#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtcctactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:62:17054:16978#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":22,"pairing_fast_overlap":62,"pairing_fast_score":0.373,"pairing_mismatches":{"(a:33)->(g:09)":52,"(a:34)->(g:06)":67,"(a:34)->(g:30)":33,"(c:33)->(g:13)":42,"(t:31)->(g:34)":35,"(t:32)->(g:10)":71,"(t:33)->(g:07)":64,"(t:34)->(g:31)":38},"sample":"29a_F260619","score":1667,"score_norm":0.806,"seq_a_single":46,"seq_ab_match":50,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatagcctt
>HELIUM_000100422_612GNAAXX:7:92:19419:19550#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":26,"pairing_fast_overlap":63,"pairing_fast_score":0.433,"pairing_mismatches":{"(a:06)->(c:30)":59,"(a:31)->(g:02)":33,"(c:07)->(a:32)":73,"(c:15)->(t:28)":70,"(c:22)->(a:26)":58,"(t:27)->(g:18)":53,"(t:31)->(g:32)":79},"sample":"26a_F040644","score":763,"score_norm":0.825,"seq_a_single":45,"seq_ab_match":52,"seq_b_single":45}
ttagccctaaacataaacattcactaaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacgtggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:15:1320:12422#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(a:08)->(c:23)":54,"(c:20)->(a:31)":32},"sample":"13a_F730603","score":1851,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatacccgt
>HELIUM_000100422_612GNAAXX:7:90:15320:19098#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtaataccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:46:17162:3642#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3490,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaact
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:7:17797:6876#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{"(c:07)->(t:02)":50,"(g:05)->(c:02)":49},"sample":"26a_F040644","score":1803,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggagcactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:119:16203:16489#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(a:34)->(c:28)":43,"(t:10)->(c:26)":50},"sample":"29a_F260619","score":3068,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcacaagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:56:8753:17490#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactgccggcgat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:72:4174:5225#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":22,"experiment":"wolf_diet","merged_sample":{"26a_F040644":22},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatcccc
>HELIUM_000100422_612GNAAXX:7:1:4932:11834#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":63,"pairing_fast_score":0.717,"pairing_mismatches":{"(a:02)->(g:34)":76,"(a:11)->(c:34)":69,"(c:02)->(a:31)":73,"(t:31)->(c:02)":37},"sample":"26a_F040644","score":1562,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagaactactagcaaca
gattaaacctcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:39:13686:12866#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"26a_F040644","score":1336,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttgcatccct
>HELIUM_000100422_612GNAAXX:7:68:5535:2001#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctgaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:2:11195:9790#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(C:02)->(T:34)":65,"(T:02)->(G:15)":39},"sample":"13a_F730603","score":1116,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaagactatgcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:11:8690:7798#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(a:02)->(t:14)":73,"(c:02)->(t:34)":81},"sample":"15a_F730814","score":2442,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactctaaggacttggcggtgctttttaccctt
>HELIUM_000100422_612GNAAXX:7:66:18851:18405#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(a:02)->(t:14)":80,"(c:02)->(a:14)":68,"(g:02)->(t:09)":76},"sample":"29a_F260619","score":237,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaatgactttgcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:93:17612:18857#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":63,"pairing_fast_score":0.783,"pairing_mismatches":{"(C:22)->(G:10)":58,"(T:34)->(G:02)":25},"sample":"26a_F040644","score":2180,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcacca
gcctgaaactcaaagggcttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:98:10635:6429#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(c:02)->(a:34)":66,"(c:02)->(t:34)":81},"sample":"29a_F260619","score":521,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgtcagagtactaccggcaat
agcttaaaactcaagggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:5:9517:19729#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":63,"pairing_fast_score":0.633,"pairing_mismatches":{"(A:02)->(G:13)":54,"(A:16)->(G:02)":24,"(A:33)->(G:02)":26,"(T:02)->(G:17)":70,"(T:33)->(A:02)":20},"sample":"26a_F040644","score":1169,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactggcaaca
gcctgaaacgcaaaggacttggcggtgctttacagccct
>HELIUM_000100422_612GNAAXX:7:17:14327:16732#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(A:02)->(G:11)":52,"(C:02)->(A:33)":69,"(C:14)->(G:10)":42,"(G:02)->(C:15)":50},"sample":"29a_F260619","score":1193,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcagt
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:29:14163:1673#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"sample":"15a_F730814","score":663,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatttgccagagtactatcggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:108:1707:8059#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(A:02)->(T:13)":66,"(A:34)->(G:02)":31,"(T:27)->(G:02)":36,"(T:31)->(G:02)":38,"(T:34)->(A:02)":27},"sample":"29a_F260619","score":210,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
agctttaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:38:7344:13615#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:02)->(T:06)":49},"sample":"13a_F730603","score":1332,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagttctaccggcaat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:6:16907:7460#0/1_sub[28..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":64,"pairing_fast_score":0.59,"pairing_mismatches":{"(a:02)->(c:15)":48,"(a:02)->(c:28)":51,"(a:02)->(c:34)":68,"(a:02)->(g:14)":43,"(a:02)->(g:15)":45,"(a:02)->(g:32)":60,"(c:02)->(g:25)":39,"(g:02)->(a:15)":44,"(t:02)->(a:26)":59},"sample":"26a_F040644","score":765,"score_norm":0.859,"seq_a_single":44,"seq_ab_match":55,"seq_b_single":44}
ttagccctaaacatagataattttacaacaaataattcgccagaggactactagcaatag
cttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:74:10186:14799#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(C:02)->(A:34)":59,"(T:32)->(G:08)":38},"sample":"13a_F730603","score":1531,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaagggcttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:11:15141:8667#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"pairing_mismatches":{"(c:27)->(g:06)":79,"(t:22)->(g:14)":77},"sample":"29a_F260619","score":3302,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaagtacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:54:10704:2877#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactagtagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:114:10746:6501#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagaatactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:117:19056:16291#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":30,"pairing_fast_overlap":63,"pairing_fast_score":0.5,"pairing_mismatches":{"(C:02)->(A:22)":51,"(C:02)->(A:29)":67,"(C:02)->(A:34)":58,"(C:02)->(G:08)":38,"(T:02)->(G:11)":64,"(T:02)->(G:26)":70},"sample":"26a_F040644","score":481,"score_norm":0.857,"seq_a_single":45,"seq_ab_match":54,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtgcgccagagtactactagcaaca
gccggaaacgcgaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:109:6220:2532#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":21,"experiment":"wolf_diet","merged_sample":{"15a_F730814":10,"29a_F260619":11},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagttctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:47:18710:11410#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"15a_F730814","score":1593,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agctcaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:24:10010:5775#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":9,"experiment":"wolf_diet","merged_sample":{"26a_F040644":9},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacatccaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:16:17663:18818#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(A:34)->(G:09)":29},"sample":"15a_F730814","score":857,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgcttcatgccctt
>HELIUM_000100422_612GNAAXX:7:9:11083:12648#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":63,"pairing_fast_score":0.733,"pairing_mismatches":{"(c:07)->(t:02)":47,"(c:20)->(a:31)":58,"(n:05)->(a:34)":72,"(t:06)->(c:02)":42},"sample":"26a_F040644","score":1487,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgctagagcactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:94:14287:20288#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":32,"pairing_fast_overlap":62,"pairing_fast_score":0.542,"pairing_mismatches":{"(a:27)->(g:02)":46,"(a:32)->(g:26)":58,"(c:02)->(g:32)":77,"(c:02)->(t:34)":81,"(c:14)->(g:02)":48,"(c:17)->(g:02)":42,"(c:26)->(a:30)":59,"(c:28)->(g:02)":53,"(g:26)->(a:02)":47,"(t:02)->(g:18)":80},"sample":"29a_F260619","score":355,"score_norm":0.806,"seq_a_single":46,"seq_ab_match":50,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagcactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:34:18733:7365#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(C:02)->(A:18)":42,"(C:02)->(G:34)":56,"(T:26)->(G:02)":22},"sample":"29a_F260619","score":531,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgacagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:105:8099:6835#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(c:06)->(a:34)":59,"(c:20)->(a:32)":66,"(c:21)->(a:18)":68,"(g:02)->(t:34)":81},"sample":"29a_F260619","score":2264,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
agcttaacactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:43:16297:17399#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"29a_F260619","score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattgttcaccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:8:9528:13218#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(t:33)->(a:09)":35},"sample":"29a_F260619","score":3001,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:69:9159:2356#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":63,"pairing_fast_score":0.983,"pairing_mismatches":{},"sample":"26a_F040644","score":4259,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaaca
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:16:15754:16899#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(A:02)->(G:15)":54,"(G:02)->(T:11)":55},"sample":"26a_F040644","score":1042,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactgtcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:50:18189:19071#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":1611,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccagcatt
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:3:17725:17195#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":62,"pairing_fast_score":0.593,"pairing_mismatches":{"(A:02)->(G:09)":49,"(A:02)->(G:10)":52,"(A:02)->(T:32)":65,"(C:02)->(G:32)":56,"(T:02)->(G:08)":71},"sample":"15a_F730814","score":587,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctgccggcaat
agcttaaaacgcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:77:19149:7092#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":62,"pairing_fast_score":0.593,"pairing_mismatches":{"(A:02)->(G:08)":33,"(A:34)->(G:02)":23,"(C:02)->(A:23)":69,"(T:02)->(G:10)":64},"sample":"13a_F730603","score":352,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaagactattcgccagagtactaccggcaat
agcgtaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:113:10533:13841#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(a:02)->(c:32)":72,"(c:25)->(t:30)":39},"sample":"29a_F260619","score":1220,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttcaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:3:1339:7213#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":63,"pairing_fast_score":0.65,"pairing_mismatches":{"(A:02)->(G:09)":48,"(C:02)->(G:13)":58,"(C:02)->(G:17)":41,"(T:34)->(G:02)":21},"sample":"26a_F040644","score":649,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcggcagagtgctactagcagca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:23:4252:12258#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":63,"pairing_fast_score":0.683,"pairing_mismatches":{"(A:07)->(C:25)":42,"(A:23)->(T:12)":48,"(C:02)->(A:33)":58,"(T:18)->(G:10)":47},"sample":"26a_F040644","score":1466,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggccgtgctttacctccct
>HELIUM_000100422_612GNAAXX:7:116:5989:5057#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(C:02)->(A:34)":67},"sample":"29a_F260619","score":1524,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcataggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:14:5884:15338#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(C:02)->(G:21)":62,"(T:02)->(G:32)":61},"sample":"26a_F040644","score":1779,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacagtcaataaacaaggatgttcgccagagtactactagcaatg
ggctaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:102:14145:8288#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(A:02)->(C:32)":54,"(A:02)->(G:10)":68,"(A:02)->(G:14)":66,"(C:02)->(A:14)":59,"(C:02)->(T:14)":65},"sample":"29a_F260619","score":1155,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttgagactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:38:19837:9316#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":63,"pairing_fast_score":0.817,"pairing_mismatches":{"(A:02)->(G:15)":58},"sample":"26a_F040644","score":1268,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcagca
gcctgaaactcaaaggacttggcggtgctttacatcccc
>HELIUM_000100422_612GNAAXX:7:116:7147:8317#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttattccctt
>HELIUM_000100422_612GNAAXX:7:117:12125:5682#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":9,"experiment":"wolf_diet","merged_sample":{"15a_F730814":5,"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:106:19425:18472#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":32,"pairing_fast_overlap":63,"pairing_fast_score":0.533,"pairing_mismatches":{"(A:02)->(G:09)":51,"(A:02)->(G:16)":48,"(C:02)->(A:07)":66,"(C:02)->(A:25)":58,"(C:34)->(G:02)":22,"(T:02)->(G:19)":70,"(T:34)->(G:02)":25},"sample":"26a_F040644","score":277,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgctgctagcaaca
gcctgaaacgcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:7:15117:2564#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtacctccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:74:16359:5324#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":9,"experiment":"wolf_diet","merged_sample":{"15a_F730814":6,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgccttataccctt
>HELIUM_000100422_612GNAAXX:7:68:13733:12120#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{},"sample":"29a_F260619","score":3408,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattactcaaacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatagcctt
>HELIUM_000100422_612GNAAXX:7:8:18430:20137#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:02)->(C:27)":54,"(A:02)->(T:34)":65,"(G:02)->(A:09)":55,"(T:34)->(G:02)":25},"sample":"15a_F730814","score":405,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccagcaat
agcttaaaactcaaaggacttggcggtgctttataccctc
>HELIUM_000100422_612GNAAXX:7:87:4048:17326#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaacaaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:101:17970:5884#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(t:02)->(a:07)":35},"sample":"15a_F730814","score":45,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaaatattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttattccctt
>HELIUM_000100422_612GNAAXX:7:4:12902:11612#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":32,"pairing_fast_overlap":62,"pairing_fast_score":0.542,"pairing_mismatches":{"(a:10)->(c:34)":54,"(c:02)->(a:31)":75,"(c:02)->(a:34)":78,"(c:02)->(t:34)":81,"(c:06)->(g:28)":62,"(c:13)->(t:07)":35,"(c:16)->(a:22)":42,"(t:02)->(c:33)":79,"(t:18)->(g:31)":56},"sample":"29a_F260619","score":1918,"score_norm":0.839,"seq_a_single":46,"seq_ab_match":52,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaactattcgacagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:115:1646:14814#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(c:02)->(a:02)":46,"(c:02)->(a:34)":74},"sample":"13a_F730603","score":10,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagmgtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:37:2111:2365#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(c:02)->(a:31)":77,"(c:02)->(t:28)":79,"(c:08)->(t:08)":41,"(t:02)->(c:32)":78},"sample":"26a_F040644","score":3486,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgycagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:82:8777:12717#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":3746,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaataattatataaacaaaattattcgccagagtactaccggcaac
agcccaaaactcaaaggacttggcggtgcttcacaccctt
>HELIUM_000100422_612GNAAXX:7:9:15894:17312#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(a:32)->(g:02)":30,"(c:34)->(a:34)":67,"(n:02)->(a:34)":72},"sample":"26a_F040644","score":1366,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgamactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:42:9039:18363#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"sample":"13a_F730603","score":804,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaactaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:106:16666:6706#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"pairing_mismatches":{"(c:02)->(t:34)":80},"sample":"26a_F040644","score":3057,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataagcaagaatgttcgccagaggactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:71:14364:8944#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":63,"pairing_fast_score":0.783,"pairing_mismatches":{"(a:13)->(c:02)":42,"(c:02)->(t:34)":80,"(c:10)->(a:02)":33},"sample":"26a_F040644","score":1088,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
tcagccctaaacataaacatccaataaacaaactaattcgcaagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:63:4784:11927#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":25,"experiment":"wolf_diet","merged_sample":{"15a_F730814":18,"29a_F260619":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaggtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:83:5561:2946#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3,"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaag
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:116:3412:11268#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":28,"pairing_fast_overlap":63,"pairing_fast_score":0.467,"pairing_mismatches":{"(A:02)->(G:07)":57,"(A:02)->(G:09)":54,"(A:02)->(G:19)":66,"(A:28)->(T:09)":51,"(A:29)->(G:26)":48,"(C:02)->(A:26)":68,"(C:17)->(T:13)":38,"(T:25)->(G:06)":35},"sample":"26a_F040644","score":823,"score_norm":0.825,"seq_a_single":45,"seq_ab_match":52,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtccgccagagtactactggcgaca
gcctggaactcaaagggcttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:6:19686:10505#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":25,"experiment":"wolf_diet","merged_sample":{"15a_F730814":16,"29a_F260619":9},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatgcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:100:14685:15065#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":28,"experiment":"wolf_diet","merged_sample":{"15a_F730814":12,"29a_F260619":16},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaacgcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:83:1732:5746#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":18,"experiment":"wolf_diet","merged_sample":{"15a_F730814":8,"29a_F260619":10},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaatgattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:34:4428:8624#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcagt
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:90:4058:17862#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagaggactactagcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:116:14653:17984#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:13)->(C:02)":43,"(C:23)->(T:02)":36,"(T:02)->(C:34)":70,"(T:23)->(G:02)":39,"(T:34)->(G:02)":21},"sample":"29a_F260619","score":52,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaatcgttcacaagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:55:18233:8570#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacgtggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:117:6925:20389#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(c:16)->(a:34)":66},"sample":"15a_F730814","score":2740,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaattattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:74:2414:20030#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"13a_F730603":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggactgggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:92:1633:11210#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggccat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:5:11975:16314#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(a:09)->(c:32)":54},"sample":"29a_F260619","score":2100,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattattcgccagagtactaccggcaat
agcttaaaactcaagggacttggcggtgctttataccctc
>HELIUM_000100422_612GNAAXX:7:19:8205:10846#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(c:31)->(g:02)":41,"(t:34)->(c:02)":37},"sample":"26a_F040644","score":1940,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaataagaatgttcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:118:2135:12859#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctcaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:46:4643:6425#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacagaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:65:18501:13769#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(g:08)->(a:08)":33},"sample":"13a_F730603","score":3118,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaractattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:111:17508:14521#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(a:02)->(g:30)":76,"(g:02)->(a:08)":75,"(g:16)->(c:02)":42},"sample":"26a_F040644","score":417,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgcgagagtactactagcaaca
gcctgaaactcaaaagacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:83:12697:9138#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(c:15)->(a:31)":68,"(c:25)->(a:32)":73},"sample":"26a_F040644","score":2925,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaataaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggtggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:25:15314:19017#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:02)->(G:18)":49,"(A:28)->(G:02)":27,"(C:02)->(A:34)":67,"(C:28)->(A:02)":26},"sample":"13a_F730603","score":282,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagcctcaaacacaaatagttatgcaaacaaaactattcgccagagtgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:16:18913:1239#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(G:02)->(C:10)":47,"(T:10)->(G:07)":39,"(T:14)->(G:19)":36},"sample":"15a_F730814","score":1154,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaatgattcgccagactactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:79:7957:9421#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(a:31)->(g:02)":33,"(c:02)->(a:30)":75,"(c:02)->(g:30)":77},"sample":"15a_F730814","score":295,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
tcagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgcgttataccctt
>HELIUM_000100422_612GNAAXX:7:58:12046:1587#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(a:02)->(c:02)":71,"(c:02)->(a:02)":68},"sample":"26a_F040644","score":2,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaaggatgttcgccagagtactactagcaaca
gcctgaamctmaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:13:6325:4377#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":6,"experiment":"wolf_diet","merged_sample":{"26a_F040644":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactattagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:100:15836:9304#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{"(T:34)->(G:02)":21},"sample":"29a_F260619","score":3076,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacatagataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:2:14358:16102#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(c:18)->(a:10)":42},"sample":"15a_F730814","score":3191,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagcaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:20:12330:11596#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttgtaccctt
>HELIUM_000100422_612GNAAXX:7:86:3769:5584#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(t:34)->(g:02)":35},"sample":"15a_F730814","score":1552,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatatgacaaaattattcgccagggtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:72:17610:8902#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaacacaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:75:4783:19300#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:34)->(G:02)":23,"(T:33)->(G:02)":21},"sample":"13a_F730603","score":1130,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgcgttataccctt
>HELIUM_000100422_612GNAAXX:7:29:2197:2024#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(g:02)->(t:34)":81,"(t:12)->(g:15)":39},"sample":"29a_F260619","score":2844,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttacccctaaacacaagtaattaatataacaaaattatgcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:89:1656:17597#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(A:02)->(G:08)":52},"sample":"15a_F730814","score":1332,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat
agcttaaagctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:5:14680:6634#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(c:02)->(a:30)":73,"(c:02)->(t:34)":80,"(c:28)->(a:02)":31,"(c:28)->(a:32)":58},"sample":"26a_F040644","score":1009,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctacacataaacattcaataaacacgaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:7:5486:6949#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":63,"pairing_fast_score":0.75,"pairing_mismatches":{"(A:02)->(T:05)":51,"(C:34)->(A:02)":22,"(T:02)->(G:06)":50},"sample":"26a_F040644","score":931,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtacgtctagcaaca
gcctgaaactcaaaggacttggcggcgctttacatccct
>HELIUM_000100422_612GNAAXX:7:102:15002:2560#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":26,"pairing_fast_overlap":63,"pairing_fast_score":0.433,"pairing_mismatches":{"(a:15)->(c:34)":52,"(a:34)->(g:02)":33,"(c:07)->(t:02)":47,"(c:09)->(a:30)":67,"(c:25)->(a:02)":51,"(g:33)->(t:02)":32,"(t:02)->(g:18)":79,"(t:30)->(g:02)":38},"sample":"26a_F040644","score":786,"score_norm":0.825,"seq_a_single":45,"seq_ab_match":52,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagcactcctagcaaca
gcctgaaactcaaaggacgtggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:92:13253:18228#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"pairing_mismatches":{"(c:21)->(t:34)":80},"sample":"26a_F040644","score":3731,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataagcattcaataaacaagaatgttcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:91:15092:21198#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":63,"pairing_fast_score":0.783,"pairing_mismatches":{"(a:31)->(g:02)":30,"(c:19)->(a:02)":31,"(g:02)->(c:33)":78,"(t:32)->(g:02)":37},"sample":"26a_F040644","score":905,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacacaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:24:17438:15641#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":40,"experiment":"wolf_diet","merged_sample":{"26a_F040644":40},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgctactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:66:5007:15261#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(G:10)":49,"(C:02)->(A:34)":59,"(C:02)->(G:34)":56},"sample":"29a_F260619","score":1902,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtgctagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:43:13909:1377#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":10,"experiment":"wolf_diet","merged_sample":{"26a_F040644":10},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaatg
gcctaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:101:6322:4903#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(T:12)":58,"(A:31)->(G:02)":31},"sample":"13a_F730603","score":70,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgtcagagtactaccggctat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:1:18764:3345#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"13a_F730603":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttagacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:96:18994:20895#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(C:02)->(A:30)":68,"(C:13)->(G:02)":39,"(T:02)->(G:26)":51,"(T:20)->(A:02)":35},"sample":"15a_F730814","score":30,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatccgccagagtacgaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:111:16947:18167#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(G:02)->(T:08)":41,"(G:34)->(A:21)":20},"sample":"13a_F730603","score":981,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattctccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:112:15020:14229#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":31,"pairing_fast_overlap":62,"pairing_fast_score":0.525,"pairing_mismatches":{"(a:02)->(c:34)":80,"(a:34)->(g:02)":33,"(c:25)->(a:12)":57,"(t:02)->(a:09)":74,"(t:28)->(g:02)":51,"(t:31)->(g:02)":35,"(t:34)->(g:02)":39},"sample":"29a_F260619","score":469,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagaacactacgagccac
agcttaaaactcaaaggacctggcggtgcttcatatccct
>HELIUM_000100422_612GNAAXX:7:107:7820:10618#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{},"sample":"13a_F730603","score":2794,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactatcggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:77:7554:16270#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":62,"pairing_fast_score":0.593,"pairing_mismatches":{"(A:02)->(T:06)":49,"(A:02)->(T:13)":48,"(C:02)->(A:08)":34,"(C:02)->(A:28)":69,"(G:02)->(C:08)":54},"sample":"29a_F260619","score":488,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagttctaccggcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:25:2569:3180#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(a:19)->(c:14)":63,"(a:34)->(c:02)":31,"(a:34)->(g:02)":33,"(a:34)->(t:02)":52},"sample":"29a_F260619","score":1279,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
agattaaaactcaaaggacttggcggcgctttataccctt
>HELIUM_000100422_612GNAAXX:7:36:3584:21256#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaagctattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:57:4707:18208#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":30,"pairing_fast_overlap":63,"pairing_fast_score":0.5,"pairing_mismatches":{"(a:02)->(c:34)":71,"(a:02)->(g:34)":76,"(a:02)->(t:23)":47,"(c:02)->(a:09)":41,"(c:02)->(a:17)":51,"(c:02)->(a:34)":58,"(c:02)->(g:27)":55,"(c:02)->(t:09)":48,"(c:02)->(t:34)":70},"sample":"26a_F040644","score":872,"score_norm":0.825,"seq_a_single":45,"seq_ab_match":52,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgacagagttctactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:3:14302:11316#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(A:02)->(C:28)":54,"(A:25)->(G:02)":24,"(C:02)->(G:22)":56,"(G:02)->(T:09)":62,"(T:02)->(G:10)":35},"sample":"15a_F730814","score":249,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtattcgccagagtactaccggcaat
atcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:54:4821:19968#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(A:02)->(C:34)":70,"(C:02)->(A:34)":66,"(T:02)->(A:26)":34,"(T:23)->(G:02)":22},"sample":"15a_F730814","score":195,"score_norm":0.823,"seq_a_single":46,"seq_ab_match":51,"seq_b_single":46}
ttagccctaaccacacgtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:18:13925:14880#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":7,"experiment":"wolf_diet","merged_sample":{"26a_F040644":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:70:11798:2668#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccttt
>HELIUM_000100422_612GNAAXX:7:79:11248:11815#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":9,"experiment":"wolf_diet","merged_sample":{"26a_F040644":9},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtaccactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:67:9849:19098#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":63,"pairing_fast_score":0.883,"pairing_mismatches":{"(A:26)->(G:23)":48,"(T:33)->(G:02)":21},"sample":"26a_F040644","score":2624,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:40:11726:14487#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":1267,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataagctattctataacaaaataattcgccagagaactactagcaaca
gcttaaaactcaaaagacttggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:18:17263:13373#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccggagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:118:6595:16048#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggcgctttataccctt
>HELIUM_000100422_612GNAAXX:7:101:8966:2415#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:29)->(G:02)":28,"(C:02)->(A:32)":68,"(G:02)->(T:09)":55,"(T:33)->(A:02)":27},"sample":"29a_F260619","score":561,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
tcagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:14371:15275#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":63,"pairing_fast_score":0.733,"pairing_mismatches":{"(A:02)->(T:07)":51,"(C:02)->(T:13)":56,"(T:26)->(G:23)":37},"sample":"26a_F040644","score":830,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtacttctagtaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:54:5891:6596#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"29a_F260619","score":2874,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgccttataccctt
>HELIUM_000100422_612GNAAXX:7:64:19383:6994#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":33,"pairing_fast_overlap":62,"pairing_fast_score":0.559,"pairing_mismatches":{"(a:33)->(g:02)":31,"(c:02)->(a:27)":69,"(c:18)->(t:09)":48,"(c:33)->(a:02)":30,"(c:33)->(a:32)":67,"(t:02)->(g:28)":80,"(t:26)->(a:02)":35,"(t:33)->(c:02)":38},"sample":"15a_F730814","score":1226,"score_norm":0.839,"seq_a_single":46,"seq_ab_match":52,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagcactaccggcaat
agcttacaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:14:1894:20471#0/1_sub[27..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":61,"pairing_fast_overlap":64,"pairing_fast_score":1,"sample":"13a_F730603","score":2310,"score_norm":1,"seq_a_single":44,"seq_ab_match":64,"seq_b_single":44}
ctagccttaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:2:2472:3175#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":2955,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagagtactactagcaaca
gcctgaaactcaaaggacttggtggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:9:5975:6484#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":63,"pairing_fast_score":0.7,"pairing_mismatches":{"(A:16)->(N:02)":28,"(C:02)->(A:21)":68,"(C:02)->(A:28)":58,"(T:02)->(G:09)":70},"sample":"26a_F040644","score":449,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaacgcaaagggcttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:77:7113:2190#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{},"sample":"15a_F730814","score":2767,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagcaattaatataacaaaattattcgccagagtaccactggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:102:15353:19491#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(A:32)->(G:02)":29,"(C:02)->(T:26)":65,"(T:02)->(G:18)":39,"(T:29)->(G:02)":21},"sample":"15a_F730814","score":157,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatgcgtcagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:29:17185:12718#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"29a_F260619","score":3832,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctagacataagctattccataacaaaattattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:115:15644:2651#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(c:28)->(a:32)":59,"(c:28)->(a:34)":68,"(t:02)->(g:34)":77},"sample":"29a_F260619","score":2621,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:80:13357:2959#0/1_sub[74..81] {"ali_dir":"right","count":12,"experiment":"wolf_diet","merged_sample":{"29a_F260619":12},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"sample":"29a_F260619"}
ccgatagg
>HELIUM_000100422_612GNAAXX:7:118:3950:17821#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(A:02)->(G:09)":52,"(C:02)->(T:34)":65,"(G:02)->(C:09)":55},"sample":"13a_F730603","score":339,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactgcccgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:49:3523:15424#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(T:02)->(G:12)":36},"sample":"13a_F730603","score":132,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctaaccttaaacacaaatagttatgcaaacaaaacgattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:14:5694:20745#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3,"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaacattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:17:3790:11309#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagttctactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:99:13155:5608#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(c:11)->(t:02)":65,"(c:34)->(a:02)":53,"(c:34)->(g:02)":43,"(t:33)->(g:02)":35},"sample":"29a_F260619","score":243,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agctcaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:116:18507:10844#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(a:32)->(t:33)":66},"sample":"29a_F260619","score":3007,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agctttaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:70:8097:4516#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaagaaacaagaatgttcaccagagtactactagcaatg
gcctaaaactcaaaggacttggcagtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:4:9681:16322#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(c:02)->(a:31)":78,"(c:34)->(g:02)":42,"(t:30)->(g:30)":64},"sample":"29a_F260619","score":1104,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcktaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:103:18504:7144#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(a:02)->(t:29)":65,"(c:02)->(a:21)":69,"(c:31)->(g:28)":56,"(g:06)->(c:29)":54,"(t:02)->(c:26)":70,"(t:34)->(g:02)":38},"sample":"29a_F260619","score":588,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttggccctaaacacaagtaattaatataacaaaattattcgccagagtactaccgccaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:20:11921:19272#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:02)->(G:09)":52,"(A:30)->(G:07)":37,"(C:02)->(G:13)":42},"sample":"13a_F730603","score":784,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcggcagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:5:14556:7029#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":63,"pairing_fast_score":0.883,"pairing_mismatches":{"(C:02)->(A:23)":68,"(T:34)->(G:10)":21},"sample":"29a_F260619","score":1812,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttatacctt
>HELIUM_000100422_612GNAAXX:7:16:5892:7877#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"sample":"29a_F260619","score":1019,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccttaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:8746:5790#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"sample":"26a_F040644","score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtacctctagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:99:6113:20429#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(a:02)->(g:34)":77,"(a:12)->(c:02)":43,"(a:28)->(g:02)":33,"(c:02)->(t:32)":65,"(c:02)->(t:34)":81},"sample":"29a_F260619","score":315,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattgttcacaagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:80:10376:19451#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":41,"experiment":"wolf_diet","merged_sample":{"15a_F730814":19,"29a_F260619":22},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:34:7467:21323#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":8,"experiment":"wolf_diet","merged_sample":{"15a_F730814":7,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatatgacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:9973:7608#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":63,"pairing_fast_score":0.75,"pairing_mismatches":{"(c:06)->(g:02)":44,"(c:10)->(a:02)":48,"(c:12)->(a:02)":67,"(g:05)->(c:02)":49},"sample":"26a_F040644","score":198,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccacagtcgtactagcaaca
gcctgacactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:73:10454:20975#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":31,"pairing_fast_overlap":62,"pairing_fast_score":0.525,"pairing_mismatches":{"(A:02)->(C:05)":66,"(A:02)->(G:08)":52,"(A:23)->(G:15)":34,"(C:02)->(A:30)":59,"(C:31)->(A:02)":26,"(T:34)->(A:02)":24,"(T:34)->(G:02)":22},"sample":"13a_F730603","score":864,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactgccggcaat
agcttcaaactcaaaggactgggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:86:14775:2223#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":12,"experiment":"wolf_diet","merged_sample":{"15a_F730814":10,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcagaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:29:10121:15634#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(c:02)->(a:26)":59,"(c:02)->(a:34)":74},"sample":"15a_F730814","score":1714,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggactcggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:61:12137:13430#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":63,"pairing_fast_score":0.733,"pairing_mismatches":{"(A:30)->(T:02)":48,"(C:28)->(G:02)":49,"(T:15)->(G:02)":47,"(T:29)->(A:02)":25},"sample":"26a_F040644","score":582,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacatagacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaagggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:8:16552:13379#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaagacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:96:12058:18750#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(c:31)->(a:02)":41},"sample":"13a_F730603","score":2223,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ctagccttaaacacaatagttatgcaaacaaaactattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:52:17727:20783#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacatcaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:36:3364:10052#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"sample":"29a_F260619","score":2912,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccctaagcacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:55:1419:14053#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(A:34)->(G:02)":27,"(C:02)->(A:32)":67,"(C:19)->(A:02)":32,"(C:32)->(A:02)":26,"(T:27)->(G:02)":21},"sample":"29a_F260619","score":1279,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacacaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:15:17486:15802#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":63,"pairing_fast_score":0.65,"pairing_mismatches":{"(c:07)->(a:02)":31,"(c:10)->(g:33)":76,"(c:18)->(a:31)":67,"(t:34)->(g:02)":50},"sample":"26a_F040644","score":578,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacattcaataaacacgaatgttcgccagagtactactagcaaca
gcctaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:81:7224:10199#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaacgcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:66:8964:2904#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(A:15)->(T:02)":52,"(C:10)->(A:02)":46,"(T:27)->(G:02)":38},"sample":"29a_F260619","score":477,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagcgtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:37:17398:13429#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:02)->(T:10)":37,"(C:02)->(A:23)":59},"sample":"15a_F730814","score":816,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaatttttcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:45:12663:16677#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(t:14)->(g:31)":76},"sample":"26a_F040644","score":1716,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacagcaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:118:14711:18853#0/1_sub[78..81] {"ali_dir":"right","ali_length":58,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":58,"pairing_fast_score":0.673,"pairing_mismatches":{},"sample":"29a_F260619","score":1404,"score_norm":0.914,"seq_a_single":50,"seq_ab_match":53,"seq_b_single":50}
ttgt
>HELIUM_000100422_612GNAAXX:7:30:11004:4740#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(A:02)->(G:10)":52,"(C:02)->(A:09)":53,"(C:02)->(G:22)":56,"(T:02)->(G:06)":39,"(T:02)->(G:07)":60,"(T:02)->(G:10)":35},"sample":"29a_F260619","score":588,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtatgcgccagagtactgacggcaag
agcttaaaactcaaaggacttggcggtgctttattccctt
>HELIUM_000100422_612GNAAXX:7:26:18498:1529#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":31,"pairing_fast_overlap":62,"pairing_fast_score":0.525,"pairing_mismatches":{"(a:02)->(c:30)":43,"(a:34)->(g:02)":29,"(c:02)->(a:23)":58,"(c:02)->(a:32)":78,"(c:02)->(t:10)":80,"(c:02)->(t:28)":51,"(g:02)->(c:34)":79,"(t:31)->(g:02)":39},"sample":"29a_F260619","score":105,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttagccctaaacacaagtaattcatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:98:9882:15208#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatacccgt
>HELIUM_000100422_612GNAAXX:7:87:18211:13787#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":15,"experiment":"wolf_diet","merged_sample":{"15a_F730814":5,"29a_F260619":10},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagcaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:119:17078:7351#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(A:02)->(G:10)":49},"sample":"29a_F260619","score":1670,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaagcacaagtaattaatataacaaaattattcgccagagtgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:2:9705:17964#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(C:02)->(A:31)":58,"(C:02)->(G:10)":62,"(T:32)->(A:02)":20},"sample":"26a_F040644","score":279,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaaca
ggctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:8:9165:18915#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactatgaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:65:12419:15574#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":6,"experiment":"wolf_diet","merged_sample":{"26a_F040644":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgttttacatccct
>HELIUM_000100422_612GNAAXX:7:31:6921:17632#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(c:02)->(a:27)":74,"(c:08)->(t:02)":48,"(c:10)->(a:02)":32,"(g:02)->(t:34)":81},"sample":"13a_F730603","score":704,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacacaactattcgccagagcactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:3:3344:2201#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":63,"pairing_fast_score":0.667,"pairing_mismatches":{"(a:02)->(c:34)":69,"(a:02)->(g:34)":76,"(c:02)->(t:28)":70,"(c:02)->(t:34)":80,"(c:14)->(a:32)":58},"sample":"29a_F260619","score":433,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactactagcaaca
gattaaacctcaaaggacttggcagcgctttatacccct
>HELIUM_000100422_612GNAAXX:7:62:19588:8344#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(a:02)->(c:32)":72,"(a:02)->(c:34)":54,"(c:02)->(g:32)":56,"(c:02)->(t:34)":81,"(t:02)->(g:24)":64},"sample":"15a_F730814","score":351,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctagacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcgtaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:30:4350:11703#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{"(a:34)->(g:18)":33},"sample":"26a_F040644","score":3363,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatacct
>HELIUM_000100422_612GNAAXX:7:19:9088:2132#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(T:33)->(A:05)":35},"sample":"15a_F730814","score":2059,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaagggcgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:112:15182:13301#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaccataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:92:13886:17277#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":63,"pairing_fast_score":0.95,"pairing_mismatches":{"(c:02)->(t:34)":80},"sample":"26a_F040644","score":1483,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaagaacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:34:12272:11244#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggcgctttacatccct
>HELIUM_000100422_612GNAAXX:7:104:18545:12154#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":32,"pairing_fast_overlap":62,"pairing_fast_score":0.542,"pairing_mismatches":{"(C:02)->(G:11)":63,"(C:14)->(A:29)":32,"(G:02)->(A:11)":56,"(T:18)->(G:18)":36,"(T:26)->(G:08)":38,"(T:32)->(G:31)":24},"sample":"29a_F260619","score":752,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaatkattcgccagagtactaccgacaat
aggttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:46:9421:7982#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":3873,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaagaacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:111:10915:12767#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":6,"experiment":"wolf_diet","merged_sample":{"26a_F040644":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacacaaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:100:12470:5788#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agtttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:101:19391:4303#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":27,"pairing_fast_overlap":63,"pairing_fast_score":0.45,"pairing_mismatches":{"(C:17)->(A:30)":34,"(C:32)->(A:19)":58,"(G:02)->(A:34)":67,"(G:02)->(C:34)":63,"(T:23)->(G:15)":53,"(T:27)->(G:02)":25},"sample":"26a_F040644","score":1416,"score_norm":0.857,"seq_a_single":45,"seq_ab_match":54,"seq_b_single":45}
tcagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcacca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:101:19319:7074#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(c:10)->(a:02)":46,"(c:21)->(a:28)":59,"(g:02)->(t:28)":81},"sample":"13a_F730603","score":779,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagcgtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:23:15204:19923#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(a:10)->(c:02)":30,"(t:02)->(c:29)":79,"(t:02)->(g:22)":80,"(t:10)->(c:02)":42,"(t:30)->(c:02)":38},"sample":"29a_F260619","score":1049,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataaaaaaattattcgtcagagtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:92:7930:1530#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(A:27)->(G:02)":52,"(A:34)->(C:02)":32,"(A:34)->(G:02)":37,"(C:02)->(G:24)":63,"(T:33)->(G:02)":35,"(T:34)->(G:02)":39},"sample":"29a_F260619","score":39,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
aggttaaaactcaagggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:21:1751:17201#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(c:02)->(g:07)":72,"(g:02)->(t:34)":81,"(t:29)->(g:16)":35},"sample":"29a_F260619","score":2067,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactgaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:79:2999:20265#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggttctttataccctt
>HELIUM_000100422_612GNAAXX:7:69:13975:12790#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(c:02)->(t:34)":65},"sample":"13a_F730603","score":1031,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcacacaaaactattcgccagagtactaccggcaat
agcttaaaactcataggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:12:11072:5516#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{},"sample":"29a_F260619","score":1421,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccagcaat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:119:10913:20597#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":62,"pairing_fast_score":0.593,"pairing_mismatches":{"(a:02)->(c:34)":70,"(c:02)->(g:34)":56,"(c:18)->(g:15)":38},"sample":"29a_F260619","score":727,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattcatataacaaaattactcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:101:6360:10835#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(a:14)->(g:17)":31,"(a:34)->(g:17)":73,"(c:13)->(t:34)":65},"sample":"29a_F260619","score":2745,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatatmacgaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:25:7110:16539#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(c:02)->(a:34)":74},"sample":"26a_F040644","score":3173,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacactcaataaacaaggatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:38:3953:18202#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(c:32)->(a:32)":43},"sample":"26a_F040644","score":3681,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccmgagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:2:15723:5445#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaccaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:120:17681:16007#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":19,"experiment":"wolf_diet","merged_sample":{"26a_F040644":19},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacacgaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:26:12470:6451#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{},"sample":"26a_F040644","score":1202,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctgaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatcccg
>HELIUM_000100422_612GNAAXX:7:31:17204:19175#0/1_sub[28..127] {"ali_dir":"left","count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"sample":"29a_F260619"}
ttagccctaaacacaagtaattaatataacaaaattatttgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:116:16497:8917#0/1_sub[28..127] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(C:02)->(G:14)":53},"sample":"13a_F730603","score":991,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactagcggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:85:7323:6139#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":9,"experiment":"wolf_diet","merged_sample":{"26a_F040644":9},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:10:1438:21106#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgktcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:107:17567:18765#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:32)->(G:21)":33,"(C:02)->(A:34)":59,"(G:02)->(A:04)":56,"(T:02)->(G:10)":36},"sample":"15a_F730814","score":716,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaatgattcgccagagtactaccgacaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:3:1768:19236#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":63,"pairing_fast_score":0.65,"pairing_mismatches":{"(C:02)->(A:10)":41,"(C:02)->(A:30)":58,"(T:02)->(G:28)":37},"sample":"26a_F040644","score":233,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatggtcgacagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:92:11939:12371#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(a:23)->(c:02)":43,"(c:06)->(t:02)":48},"sample":"15a_F730814","score":560,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgcaagagcactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:72:4084:3378#0/1_sub[28..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":64,"pairing_fast_score":0.885,"pairing_mismatches":{"(T:26)->(G:25)":36},"sample":"26a_F040644","score":1787,"score_norm":0.953,"seq_a_single":44,"seq_ab_match":61,"seq_b_single":44}
ttagccctaacatagataattttacaacaaaataattcgccagaggactactagcaatag
cttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:68:14425:5030#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(C:34)->(G:02)":30,"(T:02)->(G:15)":71},"sample":"29a_F260619","score":1536,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaacgcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:27:11553:9694#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":8,"experiment":"wolf_diet","merged_sample":{"26a_F040644":8},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaagggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:31:14834:1961#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacttaaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:102:15865:6698#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaagggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:105:8631:4802#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagctgcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:115:10883:15557#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(A:02)->(G:09)":34,"(C:02)->(A:34)":59,"(G:02)->(C:10)":55,"(T:02)->(G:18)":39},"sample":"13a_F730603","score":889,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaagctatgcgccagagtactacccgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:108:19458:4760#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":27,"pairing_fast_overlap":62,"pairing_fast_score":0.458,"pairing_mismatches":{"(a:19)->(g:02)":49,"(a:26)->(g:02)":33,"(c:02)->(t:34)":81,"(c:25)->(a:34)":75,"(c:30)->(a:02)":42,"(g:30)->(c:02)":45,"(g:32)->(t:02)":55,"(t:20)->(g:33)":77,"(t:21)->(c:02)":51,"(t:25)->(c:02)":38},"sample":"13a_F730603","score":488,"score_norm":0.839,"seq_a_single":46,"seq_ab_match":52,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccttt
>HELIUM_000100422_612GNAAXX:7:76:13250:18841#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(C:26)->(A:26)":59,"(T:31)->(G:15)":35},"sample":"29a_F260619","score":2947,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcamt
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:42:18456:2813#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(T:31)->(G:09)":70},"sample":"26a_F040644","score":3135,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttccatccct
>HELIUM_000100422_612GNAAXX:7:115:9394:14043#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(a:06)->(g:34)":77,"(c:15)->(t:02)":48},"sample":"29a_F260619","score":1100,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccctaaacacaagtaattaatataacaaaattattcgccagagcactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:6:19243:13423#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:28)->(G:02)":52,"(A:34)->(T:02)":32,"(C:02)->(A:31)":59,"(C:02)->(T:34)":65,"(C:33)->(G:02)":54},"sample":"13a_F730603","score":48,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctaccggcaat
agcttaaaactcgaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:114:5952:7883#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(T:24)->(G:29)":35,"(T:30)->(G:02)":27},"sample":"29a_F260619","score":966,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttagaccctt
>HELIUM_000100422_612GNAAXX:7:50:1357:8953#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":63,"pairing_fast_score":0.75,"pairing_mismatches":{"(c:02)->(a:32)":77,"(n:04)->(a:27)":51,"(n:04)->(c:34)":52,"(n:04)->(t:31)":53,"(n:05)->(t:32)":50},"sample":"26a_F040644","score":1171,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacattcaataaccaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:23:6873:9774#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":28,"pairing_fast_overlap":62,"pairing_fast_score":0.475,"pairing_mismatches":{"(C:02)->(A:33)":68,"(C:02)->(T:31)":65,"(C:06)->(A:34)":44,"(C:16)->(T:34)":48,"(C:18)->(A:28)":29,"(T:02)->(G:32)":56},"sample":"29a_F260619","score":1556,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaccacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:109:5074:12821#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":31,"pairing_fast_overlap":63,"pairing_fast_score":0.517,"pairing_mismatches":{"(a:26)->(g:02)":33,"(a:30)->(g:02)":30,"(c:02)->(a:32)":74,"(c:10)->(g:09)":62,"(c:10)->(t:02)":38,"(c:21)->(g:02)":41,"(t:02)->(g:13)":70,"(t:16)->(c:02)":37},"sample":"26a_F040644","score":310,"score_norm":0.857,"seq_a_single":45,"seq_ab_match":54,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtccgccagagtactactagcaaca
gcctgaaacgcaaaggacttggcggtgctttacagccct
>HELIUM_000100422_612GNAAXX:7:43:10405:17704#0/1_sub[28..127] {"ali_dir":"left","count":11,"experiment":"wolf_diet","merged_sample":{"13a_F730603":11},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ctagccttaaacacaaatagctatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:74:7842:18269#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:97:7993:6078#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"13a_F730603":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccctaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:90:3084:16108#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":32,"experiment":"wolf_diet","merged_sample":{"26a_F040644":32},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaagggcttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:93:6051:12458#0/1_sub[28..127] {"ali_dir":"left","count":27,"experiment":"wolf_diet","merged_sample":{"15a_F730814":16,"29a_F260619":11},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaagctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:53:7051:5623#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgatcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:53:15169:15350#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":12,"experiment":"wolf_diet","merged_sample":{"13a_F730603":12},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaagctattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:12:19602:12300#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(C:02)->(A:24)":58},"sample":"26a_F040644","score":641,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagtaaca
gcctgaaactcagaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:4:16910:11013#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(a:21)->(t:02)":39,"(c:02)->(a:32)":75,"(g:02)->(a:15)":76,"(g:02)->(t:34)":81},"sample":"13a_F730603","score":1600,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactatacgccagagtactaccggcaat
agcttaaaactcaaaagacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:106:2362:17222#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{"(G:02)->(C:15)":40},"sample":"26a_F040644","score":955,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttccccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:73:13423:6867#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"13a_F730603":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccgacaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:109:19367:11741#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(A:02)->(C:26)":54,"(A:02)->(T:34)":65,"(C:02)->(A:30)":59,"(C:02)->(G:27)":56,"(T:24)->(G:07)":35},"sample":"15a_F730814","score":1309,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcagaggacttggcggtgcttgataccctt
>HELIUM_000100422_612GNAAXX:7:23:6103:3418#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:47:16435:10123#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaga
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:9:2970:2232#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(a:08)->(c:30)":43,"(n:02)->(c:34)":72},"sample":"15a_F730814","score":1783,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagcaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtactttataccctt
>HELIUM_000100422_612GNAAXX:7:72:18337:8148#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":62,"pairing_fast_score":0.983,"pairing_mismatches":{},"sample":"15a_F730814","score":1692,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaaataatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcagaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:110:19182:18902#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(c:31)->(g:22)":42,"(c:34)->(a:31)":75,"(c:34)->(g:34)":77,"(t:02)->(g:18)":80,"(t:30)->(a:09)":38},"sample":"13a_F730603","score":2256,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaacgsacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:102:15249:17398#0/1_sub[28..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":61,"pairing_fast_overlap":64,"pairing_fast_score":1,"sample":"26a_F040644","score":2115,"score_norm":1,"seq_a_single":44,"seq_ab_match":64,"seq_b_single":44}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacagcaacag
cctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:120:19121:17559#0/1_sub[28..127] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":63,"pairing_fast_score":0.6,"pairing_mismatches":{"(A:02)->(G:11)":49,"(C:02)->(A:32)":59,"(C:02)->(T:34)":65,"(C:21)->(A:08)":32},"sample":"13a_F730603","score":134,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ctagccttaaacacaaatagttatgcaaacacaactattcgccagagtgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:119:14424:17028#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:02)->(G:23)":58,"(C:02)->(G:11)":53,"(C:02)->(G:30)":56},"sample":"15a_F730814","score":1516,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactagcggcgat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:76:4066:21027#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":63,"pairing_fast_score":0.667,"pairing_mismatches":{"(a:08)->(c:02)":56,"(a:34)->(g:02)":34,"(c:34)->(g:02)":29,"(t:02)->(g:15)":79,"(t:31)->(c:02)":37},"sample":"26a_F040644","score":728,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagaaaca
gcctgaaactcaaaggacgtggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctacctgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:85:8043:4624#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{"(t:35)->(g:18)":37},"sample":"26a_F040644","score":3186,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaaaaatgttcgccagagtactactaacaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:70:8301:14823#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"13a_F730603":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttagcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:103:8878:18405#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"15a_F730814","seq_a_single":45,"seq_b_single":45}
tagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:18:18798:5806#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":4130,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggggctttacatccct
>HELIUM_000100422_612GNAAXX:7:58:3691:18582#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"29a_F260619","score":1591,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaactgaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:10:10003:18815#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(a:06)->(t:32)":48,"(a:34)->(g:02)":31,"(c:08)->(a:34)":75,"(g:02)->(t:34)":81},"sample":"29a_F260619","score":1883,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacaaaagtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:93:18144:4836#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":63,"pairing_fast_score":0.583,"pairing_mismatches":{"(a:24)->(t:02)":47,"(c:06)->(a:31)":58,"(c:30)->(g:02)":41,"(g:02)->(t:30)":80,"(g:08)->(a:02)":34,"(g:10)->(c:31)":63},"sample":"26a_F040644","score":472,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacataaacatacaataaacaagagtgtgcgccagagaactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:28:10296:21171#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":63,"pairing_fast_score":0.567,"pairing_mismatches":{"(C:06)->(A:31)":58,"(C:16)->(A:02)":31,"(G:12)->(C:30)":63,"(G:19)->(A:28)":61,"(T:21)->(G:02)":38,"(T:33)->(A:02)":21},"sample":"26a_F040644","score":1280,"score_norm":0.857,"seq_a_single":45,"seq_ab_match":54,"seq_b_single":45}
ttagccctaaacataaacattcaataaacacgaatgttcgccagagtactactagcaaca
acctgaaactcaagggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:115:3218:7489#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"sample":"13a_F730603","score":983,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttgggggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:71:2666:17763#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":3896,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagtcttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:70:4933:4560#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:02)->(T:33)":65},"sample":"29a_F260619","score":2289,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtattaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:73:2959:18329#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctaaccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:15:8108:8983#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(T:22)->(G:29)":35},"sample":"15a_F730814","score":1259,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:97:18179:9968#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"sample":"29a_F260619","score":2086,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaaataattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcgttgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:109:12823:5853#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"sample":"29a_F260619","score":1362,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttggccctaaacacaagtaattaatataacaagattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:68:19723:4321#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":26,"pairing_fast_overlap":62,"pairing_fast_score":0.441,"pairing_mismatches":{"(a:02)->(g:31)":77,"(a:16)->(t:10)":33,"(a:21)->(c:12)":31,"(a:30)->(t:10)":52,"(c:02)->(a:21)":66,"(c:02)->(a:23)":69,"(c:02)->(a:34)":74,"(t:02)->(g:11)":64,"(t:13)->(g:22)":39},"sample":"13a_F730603","score":1072,"score_norm":0.806,"seq_a_single":46,"seq_ab_match":50,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactatgcgccagagtactaccggcaat
agcgtaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:32:15168:13756#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":4172,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaccacaaatagttatgcaaacaaaactattcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:45:8094:16674#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(T:28)->(G:28)":35},"sample":"15a_F730814","score":1767,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaaktattcgccagagtactaccagcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:107:2103:10677#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":20,"experiment":"wolf_diet","merged_sample":{"13a_F730603":20},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:21:4897:4980#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaccattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:1:16623:15039#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccatagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:68:15800:19845#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacctggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:68:2026:8658#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(t:34)->(g:34)":64},"sample":"13a_F730603","score":3148,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcktaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:81:16265:19901#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":63,"pairing_fast_score":0.583,"pairing_mismatches":{"(a:30)->(g:02)":33,"(c:02)->(a:26)":67,"(c:02)->(a:35)":73,"(c:08)->(t:02)":47,"(c:30)->(g:02)":41,"(g:02)->(c:34)":63,"(g:23)->(t:02)":32},"sample":"26a_F040644","score":152,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
tcagccctaaacataaacattcaataaacaggaatgttcgccagagcactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:55:8389:5703#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(a:15)->(t:15)":39},"sample":"15a_F730814","score":3470,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatwcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:114:6357:17076#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{},"sample":"15a_F730814","score":1760,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtactttataccctt
>HELIUM_000100422_612GNAAXX:7:50:7300:21068#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(C:02)->(A:34)":67,"(C:32)->(A:32)":32},"sample":"15a_F730814","score":1221,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacacgtaattaatataacamaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:1:8878:9269#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:02)->(T:07)":49,"(A:33)->(G:15)":33,"(C:02)->(A:06)":52,"(G:02)->(T:12)":55},"sample":"13a_F730603","score":250,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagttctacctgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:18:5765:13775#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":63,"pairing_fast_score":0.65,"pairing_mismatches":{"(A:02)->(C:34)":63,"(C:02)->(A:34)":67,"(G:02)->(A:14)":55,"(G:25)->(T:02)":36,"(T:21)->(G:02)":37},"sample":"26a_F040644","score":93,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
tcagccctaaacataaacattcaataaacaagaatgttcgccagagtactactaacaaca
acctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:98:18498:21034#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(a:02)->(c:31)":72,"(a:06)->(c:02)":43,"(c:02)->(a:34)":75,"(c:11)->(a:02)":61},"sample":"29a_F260619","score":51,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgcaagagtactaccggcaat
cgcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:30:18241:4387#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"sample":"29a_F260619","score":1630,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaagattattcgccacagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:111:12298:15702#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(A:02)->(G:07)":58,"(A:02)->(G:15)":49,"(C:02)->(A:34)":67,"(C:02)->(T:34)":65},"sample":"13a_F730603","score":484,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctaccggcgat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:69:1848:10784#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":17,"experiment":"wolf_diet","merged_sample":{"15a_F730814":8,"29a_F260619":9},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
atcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:21:6999:18567#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":29,"pairing_fast_overlap":62,"pairing_fast_score":0.492,"pairing_mismatches":{"(a:21)->(g:02)":67,"(a:34)->(c:02)":31,"(a:34)->(g:02)":29,"(c:28)->(g:02)":42,"(c:34)->(a:02)":30,"(g:32)->(t:02)":55,"(t:33)->(g:02)":35},"sample":"29a_F260619","score":446,"score_norm":0.806,"seq_a_single":46,"seq_ab_match":50,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctgtatacccgt
>HELIUM_000100422_612GNAAXX:7:1:17335:3440#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":10,"experiment":"wolf_diet","merged_sample":{"15a_F730814":7,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaagattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:36:6727:16123#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(a:11)->(c:30)":63,"(t:19)->(a:19)":35,"(t:29)->(g:16)":64},"sample":"29a_F260619","score":2995,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaaataatataacaaaawtattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:8:11150:3377#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":6,"experiment":"wolf_diet","merged_sample":{"26a_F040644":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccggagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:97:9988:16961#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(C:02)->(G:34)":56,"(T:02)->(G:25)":35},"sample":"15a_F730814","score":395,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtattcgccagagtactaccggcaat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:22:19336:9212#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(c:34)->(a:24)":33},"sample":"13a_F730603","score":2373,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaacactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:1:15993:20360#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacctgcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:103:1205:6990#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:15273:14157#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(T:05)":52,"(G:02)->(C:14)":55,"(T:02)->(C:07)":51,"(T:32)->(G:07)":35},"sample":"15a_F730814","score":1473,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaagattattcgccagagtacctcccgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:99:14468:19242#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(c:18)->(a:34)":75,"(c:19)->(t:34)":65,"(c:21)->(a:34)":67,"(c:22)->(a:02)":33,"(c:25)->(a:34)":59,"(c:28)->(t:34)":81},"sample":"15a_F730814","score":1637,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctacacacaagtaattaatataacaacattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:92:14716:19849#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(a:10)->(c:02)":40,"(c:02)->(a:13)":67,"(c:02)->(a:26)":69,"(c:02)->(a:34)":75,"(c:02)->(g:28)":80,"(c:02)->(g:33)":56,"(c:02)->(g:34)":77,"(c:12)->(t:02)":39},"sample":"13a_F730603","score":422,"score_norm":0.839,"seq_a_single":46,"seq_ab_match":52,"seq_b_single":46}
ctagccttaaacacaaatagtcatgcaaacaaaactatcagccagagtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:92:11935:18409#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:02)->(T:07)":49,"(A:33)->(T:11)":34},"sample":"13a_F730603","score":1000,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcaccagagttctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:59:4820:2747#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(a:09)->(c:02)":54,"(c:34)->(a:02)":42,"(t:28)->(g:02)":35},"sample":"29a_F260619","score":1296,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactacaggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:21:14395:7809#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":63,"pairing_fast_score":0.733,"pairing_mismatches":{"(C:02)->(A:33)":68,"(C:10)->(A:02)":28,"(G:25)->(T:02)":32},"sample":"26a_F040644","score":761,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
tcagccctaaacataaacattcaataaccaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:40:19586:2959#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatatatcaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:37:3760:15390#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(c:25)->(g:13)":44,"(c:29)->(g:34)":76},"sample":"26a_F040644","score":2899,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccacagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:111:7482:7435#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccacagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:39:13951:9449#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":63,"pairing_fast_score":0.583,"pairing_mismatches":{"(A:02)->(G:10)":48,"(C:33)->(A:02)":22,"(C:34)->(A:02)":29,"(T:02)->(G:09)":70,"(T:34)->(A:02)":25},"sample":"26a_F040644","score":219,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgctactagcaaca
gcctgaaacgcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:113:18143:21104#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(A:02)->(C:34)":54,"(C:02)->(A:28)":59,"(C:02)->(G:11)":50,"(T:02)->(G:11)":60,"(T:34)->(G:02)":27},"sample":"29a_F260619","score":611,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtagtaccggcaag
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:1:8606:12274#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":30,"pairing_fast_overlap":62,"pairing_fast_score":0.508,"pairing_mismatches":{"(A:02)->(C:34)":72,"(C:02)->(A:34)":67,"(C:02)->(G:34)":56,"(G:02)->(A:25)":55,"(T:02)->(G:07)":35,"(T:02)->(G:09)":48},"sample":"29a_F260619","score":708,"score_norm":0.839,"seq_a_single":46,"seq_ab_match":52,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaagtattcgccagaggactaccagcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:37:15203:11395#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":8,"experiment":"wolf_diet","merged_sample":{"13a_F730603":8},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaagggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:111:2380:10482#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"29a_F260619","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactactagcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:34:6747:7647#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:02)->(T:10)":49,"(T:34)->(A:07)":25},"sample":"29a_F260619","score":1587,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaatcattcgccagagttctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:76:5974:12694#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(G:13)":58,"(C:02)->(A:24)":59,"(C:02)->(G:32)":56,"(G:23)->(A:08)":47},"sample":"29a_F260619","score":1845,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcgat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:87:15245:17364#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(A:02)->(C:34)":54,"(A:02)->(G:14)":52,"(A:02)->(G:16)":69,"(A:26)->(G:08)":34,"(C:02)->(G:34)":56},"sample":"15a_F730814","score":1476,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat
agcttaaagctcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:111:5065:12850#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":63,"pairing_fast_score":0.817,"pairing_mismatches":{"(c:07)->(a:02)":54,"(c:15)->(a:02)":41},"sample":"29a_F260619","score":1091,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactactcgcaaca
gattaaacctcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:100:13971:16163#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":63,"pairing_fast_score":0.633,"pairing_mismatches":{"(a:07)->(c:02)":39,"(a:15)->(c:02)":42,"(a:33)->(t:02)":30,"(c:02)->(a:34)":74,"(c:02)->(t:34)":80,"(c:08)->(t:02)":38,"(c:16)->(g:34)":61},"sample":"26a_F040644","score":623,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtcagcaagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:80:8764:7469#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(t:08)->(g:02)":76},"sample":"26a_F040644","score":28,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaagtacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:53:3934:1337#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:02)->(G:10)":49},"sample":"15a_F730814","score":1125,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:9:16006:1865#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":63,"pairing_fast_score":0.7,"pairing_mismatches":{"(c:02)->(a:32)":68,"(c:11)->(a:27)":58,"(n:02)->(a:34)":72,"(t:34)->(c:07)":37},"sample":"26a_F040644","score":1766,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccgtaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:47:4240:4530#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:58:13540:3181#0/1_sub[28..127] {"ali_dir":"left","count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603","score_norm":0.984}
ctagccttaaacacaaatagttatgcaaacaaaactattcaccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:48:14465:4499#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(A:32)->(G:09)":49,"(A:32)->(G:19)":34},"sample":"13a_F730603","score":2333,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaattagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:4:1667:10015#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:02)->(G:22)":49,"(T:26)->(G:17)":36},"sample":"13a_F730603","score":1453,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctaccggcaat
agcttaaaacccaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:8:8060:4751#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(C:02)->(G:26)":72},"sample":"13a_F730603","score":1002,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactgaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:99:13083:9739#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(A:32)->(G:09)":28,"(A:32)->(G:13)":32,"(C:02)->(G:18)":50,"(C:02)->(T:34)":65,"(C:34)->(G:07)":30,"(T:34)->(A:22)":21},"sample":"15a_F730814","score":1020,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtagtaccggcaat
agcttaaaactcaaaggacgtggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:76:10530:11312#0/1_sub[28..126] {"ali_dir":"left","count":43,"experiment":"wolf_diet","merged_sample":{"26a_F040644":43},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:29:15294:15411#0/1_sub[28..127] {"ali_dir":"left","count":15,"experiment":"wolf_diet","merged_sample":{"13a_F730603":15},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ctagccttaaacacaaatagttatgcaaacaaaactatgcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:3:13228:16162#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":9,"experiment":"wolf_diet","merged_sample":{"13a_F730603":9},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:55:14857:15240#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{},"sample":"29a_F260619","score":3370,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagcactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:100:7802:15495#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttgaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:85:14507:19124#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":63,"pairing_fast_score":0.65,"pairing_mismatches":{"(a:18)->(g:02)":42,"(c:02)->(a:32)":68,"(c:02)->(a:34)":74,"(c:02)->(g:33)":76,"(c:16)->(a:34)":58,"(t:02)->(c:34)":69},"sample":"26a_F040644","score":353,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:8:8585:18698#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacatacaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:12:17779:3034#0/1_sub[78..81] {"ali_dir":"right","ali_length":58,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":58,"pairing_fast_score":0.909,"pairing_mismatches":{},"sample":"29a_F260619","score":25,"score_norm":0.966,"seq_a_single":50,"seq_ab_match":56,"seq_b_single":50}
tttg
>HELIUM_000100422_612GNAAXX:7:3:14818:6698#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"pairing_mismatches":{"(c:02)->(g:34)":77,"(c:02)->(t:34)":81},"sample":"13a_F730603","score":1810,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaaaagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:104:9960:10217#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":63,"pairing_fast_score":0.7,"pairing_mismatches":{"(A:02)->(T:07)":54,"(A:02)->(T:08)":57,"(A:21)->(T:07)":28},"sample":"26a_F040644","score":1027,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacttgctaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:118:9408:17106#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:02)->(T:07)":52,"(C:02)->(G:33)":56,"(G:21)->(T:06)":41,"(T:34)->(A:07)":21},"sample":"15a_F730814","score":1277,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtacttccggcaat
agcttaaaacccaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:6:8468:2353#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaagacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:79:6214:21271#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat
agcttaaaactcaaaggactgggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:105:5248:16600#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":63,"pairing_fast_score":0.683,"pairing_mismatches":{"(A:02)->(G:06)":51,"(G:02)->(A:09)":55,"(G:02)->(C:32)":63},"sample":"26a_F040644","score":238,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactgctaacaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:15:17062:15943#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":63,"pairing_fast_score":0.917,"pairing_mismatches":{"(c:07)->(a:09)":41},"sample":"26a_F040644","score":3432,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgacagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:28:18701:11778#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaaccttcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:108:16275:18283#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(C:02)->(A:32)":68,"(C:02)->(G:10)":56,"(G:02)->(T:19)":62,"(T:02)->(G:08)":39,"(T:32)->(A:02)":21},"sample":"29a_F260619","score":964,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatgcgccagagtactgccggcaat
atcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:72:5181:18468#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":3902,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagacttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggaggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:96:13100:7447#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(a:10)->(c:02)":42,"(c:25)->(a:24)":58},"sample":"26a_F040644","score":1616,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcacca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:118:13899:15858#0/1_sub[28..127] {"ali_dir":"left","count":32,"experiment":"wolf_diet","merged_sample":{"13a_F730603":32},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:74:18231:8655#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":9,"experiment":"wolf_diet","merged_sample":{"26a_F040644":9},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaatattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:61:6514:4042#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcagaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:108:15653:17384#0/1_sub[28..127] {"ali_dir":"left","count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"sample":"15a_F730814"}
ttagccctaaacacaagtaattaatataacaaagttattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:62:19764:18526#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
aacttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:42:2391:6987#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(a:02)->(g:34)":76},"sample":"26a_F040644","score":2891,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaaaataattcgccagagaactactagcaaca
gcttaaaactcaaaggacgtggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:62:15811:19585#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtacgaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:41:14341:7850#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(C:02)->(T:10)":57},"sample":"29a_F260619","score":1863,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggtaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:19:9050:13030#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(C:02)->(T:34)":65,"(G:02)->(T:09)":55},"sample":"13a_F730603","score":1230,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacctgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:90:18978:12189#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcgtgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:58:18698:2173#0/1_sub[27..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":64,"pairing_fast_score":0.754,"pairing_mismatches":{"(A:02)->(C:19)":60,"(A:34)->(T:02)":31,"(G:34)->(A:02)":32,"(T:32)->(A:02)":21},"sample":"26a_F040644","score":549,"score_norm":0.922,"seq_a_single":44,"seq_ab_match":59,"seq_b_single":44}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacc
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:115:8142:17162#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":63,"pairing_fast_score":0.583,"pairing_mismatches":{"(A:02)->(C:25)":54,"(C:02)->(G:09)":41,"(G:34)->(C:02)":32,"(T:02)->(C:16)":50},"sample":"26a_F040644","score":391,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcggcagagtaccactcgcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:94:4254:19676#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":63,"pairing_fast_score":0.617,"pairing_mismatches":{"(A:34)->(G:02)":45,"(G:29)->(T:02)":46,"(G:34)->(A:02)":32,"(T:34)->(A:02)":25,"(T:34)->(G:02)":35},"sample":"26a_F040644","score":992,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcamaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:85:14708:4489#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"pairing_mismatches":{},"sample":"26a_F040644","score":1365,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaata
gcttaaaactcaaaggacttggtggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:19:6488:20199#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattaaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:29:11728:15797#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(a:08)->(g:34)":77},"sample":"15a_F730814","score":3221,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttcgccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:61:14201:20987#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:23)->(C:10)":52,"(C:24)->(A:31)":67,"(T:31)->(G:02)":36},"sample":"29a_F260619","score":1235,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaagggactgggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:2:12987:20515#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(A:02)->(T:31)":36,"(C:02)->(T:31)":65,"(T:15)->(G:25)":21},"sample":"15a_F730814","score":219,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaagtaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:65:5481:2645#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(T:02)->(G:18)":48,"(T:16)->(G:02)":35,"(T:29)->(G:02)":27,"(T:30)->(A:02)":21},"sample":"29a_F260619","score":451,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagaggactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:115:12230:9032#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(a:02)->(g:33)":77,"(a:07)->(c:02)":40,"(c:12)->(g:02)":45,"(c:15)->(g:02)":42},"sample":"15a_F730814","score":1094,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattagccacagtactaccggcaat
aacttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:19:2854:6315#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"15a_F730814":5,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacacaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:43:10961:20685#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":76,"experiment":"wolf_diet","merged_sample":{"15a_F730814":36,"29a_F260619":40},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:73:8633:8194#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":10,"experiment":"wolf_diet","merged_sample":{"15a_F730814":8,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcaccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:79:12752:13649#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":30,"pairing_fast_overlap":62,"pairing_fast_score":0.508,"pairing_mismatches":{"(A:31)->(G:02)":32,"(A:31)->(T:02)":49,"(C:02)->(A:24)":69,"(C:02)->(A:25)":67,"(C:02)->(T:21)":65,"(T:22)->(G:02)":36,"(T:29)->(A:02)":39},"sample":"29a_F260619","score":425,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctg
>HELIUM_000100422_612GNAAXX:7:5:4004:9294#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:02)->(G:06)":69,"(T:26)->(G:31)":35},"sample":"29a_F260619","score":1327,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtattcgccagagtactaccggcaat
agcttaaagctcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:99:14085:8423#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagaatactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:41:3113:15845#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":28,"pairing_fast_overlap":62,"pairing_fast_score":0.475,"pairing_mismatches":{"(A:07)->(T:02)":39,"(A:26)->(G:02)":52,"(A:34)->(G:02)":31,"(C:02)->(A:30)":69,"(C:02)->(A:31)":67,"(C:19)->(G:25)":56,"(C:30)->(G:10)":57,"(C:34)->(A:02)":30,"(T:29)->(G:02)":36,"(T:34)->(A:02)":21},"sample":"29a_F260619","score":92,"score_norm":0.823,"seq_a_single":46,"seq_ab_match":51,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatacgccagagtactaccggcaat
agcttaaaactcaagggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:70:12503:15796#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaagaacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:24:16005:14236#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(a:10)->(c:34)":43,"(c:08)->(t:30)":65},"sample":"15a_F730814","score":1571,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagaaattaatataacaaaattattcaccagagtactaccggcaag
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:79:10778:7634#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":63,"pairing_fast_score":0.917,"pairing_mismatches":{"(C:02)->(G:15)":56,"(G:02)->(T:09)":55},"sample":"26a_F040644","score":1703,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacagtcaataaacaaggatgttcgccagagtactactatgaatg
gcctaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:108:17000:6439#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(a:25)->(c:26)":54},"sample":"29a_F260619","score":2712,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgctagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:96:6520:14775#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(a:06)->(t:02)":39,"(c:02)->(a:31)":74,"(c:02)->(t:34)":81,"(c:22)->(a:02)":42,"(t:26)->(a:02)":35},"sample":"29a_F260619","score":214,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatacgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:71:10176:5448#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":63,"pairing_fast_score":0.667,"pairing_mismatches":{"(C:02)->(A:34)":58,"(C:02)->(T:29)":64,"(C:33)->(A:02)":23,"(T:28)->(A:02)":37,"(T:29)->(G:02)":34,"(T:33)->(A:02)":21},"sample":"29a_F260619","score":650,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactactagcaaca
gattaaacctcaaaggacgtggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:63:15217:5994#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{"(c:18)->(t:02)":37},"sample":"26a_F040644","score":1710,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgctcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:81:7903:21237#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":63,"pairing_fast_score":0.667,"pairing_mismatches":{"(C:02)->(A:31)":58,"(C:02)->(G:06)":56,"(C:34)->(A:02)":22,"(G:31)->(T:02)":36,"(T:31)->(G:02)":37,"(T:34)->(G:02)":25},"sample":"26a_F040644","score":469,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactaggaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:76:17751:20258#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":26,"pairing_fast_overlap":63,"pairing_fast_score":0.433,"pairing_mismatches":{"(a:24)->(g:02)":35,"(c:02)->(g:34)":76,"(c:02)->(t:32)":80,"(c:14)->(g:02)":46,"(c:18)->(a:02)":32,"(c:18)->(t:25)":64,"(c:22)->(a:24)":66,"(c:30)->(g:02)":41,"(t:26)->(g:02)":37},"sample":"26a_F040644","score":717,"score_norm":0.825,"seq_a_single":45,"seq_ab_match":52,"seq_b_single":45}
ttagccctaaacatagataattttacaacaacataattcgccagacgactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:115:12450:15002#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(C:02)->(A:34)":67,"(C:02)->(G:10)":42,"(C:02)->(G:28)":56,"(G:02)->(C:10)":55},"sample":"13a_F730603","score":647,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcggcagagtactacccgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:57:4186:18599#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaatgaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:40:15984:4911#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":16,"experiment":"wolf_diet","merged_sample":{"26a_F040644":16},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:55:1899:8098#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(A:23)->(G:11)":35,"(C:02)->(A:28)":68,"(T:17)->(G:21)":37},"sample":"26a_F040644","score":2330,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataagctattctataacaaaataagtcgccagagaactactagcaaca
gcttaaaactcaaaggacttggcggtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:7:3174:16755#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":10,"experiment":"wolf_diet","merged_sample":{"26a_F040644":10},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtacttctagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:42:9138:11222#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtaccaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:106:7632:8306#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctctacatccct
>HELIUM_000100422_612GNAAXX:7:118:10007:4749#0/1_sub[28..127] {"ali_dir":"left","count":4,"experiment":"wolf_diet","merged_sample":{"13a_F730603":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ctagccttaaacacaaatagttgtgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:44:2789:9813#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":63,"pairing_fast_score":0.783,"pairing_mismatches":{"(c:23)->(t:23)":80,"(c:26)->(t:02)":41,"(g:22)->(t:02)":46},"sample":"26a_F040644","score":801,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggactyggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:109:19171:17424#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1,"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"seq_a_single":45,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggacttctagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:56:9720:7287#0/1_sub[28..126] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":64,"pairing_fast_score":0.623,"pairing_mismatches":{"(A:02)->(G:11)":51,"(A:02)->(G:14)":58,"(T:02)->(C:30)":63,"(T:28)->(G:02)":35},"sample":"26a_F040644","score":698,"score_norm":0.906,"seq_a_single":44,"seq_ab_match":58,"seq_b_single":44}
ttggccctaaacataaacattcaataaacaagaatgttcgccagagtactgctagcagca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:81:16002:9395#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(c:34)->(a:08)":41},"sample":"26a_F040644","score":3169,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctaaaactcaaaggacttggcgatgctttacatccct
>HELIUM_000100422_612GNAAXX:7:63:7875:11278#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggatttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:99:12873:11318#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(A:02)->(T:02)":51,"(C:02)->(A:34)":67},"sample":"26a_F040644","score":27,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactwctagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:7:10221:13533#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(A:02)->(G:05)":52,"(A:34)->(G:02)":23,"(C:15)->(A:02)":28},"sample":"13a_F730603","score":567,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcacacaaaactattcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:13:4725:5598#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"sample":"13a_F730603","score":1816,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaagtattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:54:7444:11885#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3682,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacatccaataaacaagaatgttcgccagagtactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:98:11789:10544#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(A:02)->(G:21)":48,"(T:26)->(A:02)":25},"sample":"26a_F040644","score":131,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgctactagcaaca
gcctgaaactcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:4:6214:17341#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(C:02)->(G:28)":56,"(C:02)->(T:34)":65,"(T:02)->(G:10)":35},"sample":"15a_F730814","score":851,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctc
>HELIUM_000100422_612GNAAXX:7:28:7199:19024#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:02)->(C:32)":54,"(A:02)->(G:10)":52,"(G:02)->(T:19)":55},"sample":"13a_F730603","score":530,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactgcctgcaat
agcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:98:2444:20028#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":62,"pairing_fast_score":0.593,"pairing_mismatches":{"(a:02)->(g:32)":77,"(a:21)->(g:02)":49,"(a:31)->(g:02)":34,"(a:34)->(g:02)":31,"(c:02)->(a:33)":78,"(c:21)->(a:02)":46,"(t:02)->(c:32)":79,"(t:21)->(g:02)":39,"(t:34)->(g:02)":36},"sample":"13a_F730603","score":235,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ctagccttaaacacgaatagttatgcaaacaaaactattcgccagcgtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:11:5417:14170#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcacaagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:107:15252:19137#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":24,"pairing_fast_overlap":62,"pairing_fast_score":0.407,"pairing_mismatches":{"(A:30)->(G:12)":37,"(C:02)->(T:34)":65,"(G:02)->(A:06)":47,"(G:02)->(T:11)":56,"(T:02)->(G:15)":48,"(T:19)->(G:18)":39,"(T:28)->(G:14)":35,"(T:34)->(G:28)":25,"(T:34)->(G:30)":21},"sample":"29a_F260619","score":693,"score_norm":0.806,"seq_a_single":46,"seq_ab_match":50,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagaagactaccgtcaat
agcttaaaactcaaagggcttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:118:2621:21156#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":27,"pairing_fast_overlap":62,"pairing_fast_score":0.458,"pairing_mismatches":{"(A:02)->(G:18)":52,"(A:32)->(G:02)":32,"(A:34)->(G:02)":28,"(C:34)->(G:02)":40,"(G:30)->(C:02)":41,"(G:32)->(T:02)":24,"(T:34)->(G:02)":38},"sample":"15a_F730814","score":44,"score_norm":0.806,"seq_a_single":46,"seq_ab_match":50,"seq_b_single":46}
ttagccctaaacacaagtaattagtataacaaaattattcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:77:17898:19592#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaataattcgccagaggactactagcaat
agcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:89:3726:16704#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatcccg
>HELIUM_000100422_612GNAAXX:7:119:4170:13200#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(T:27)->(G:28)":38,"(T:34)->(G:02)":20},"sample":"26a_F040644","score":701,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtgcgccagagtactactagcaaca
gcctgaaacttaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:32:4908:16517#0/1_sub[78..81] {"ali_dir":"right","ali_length":58,"count":7,"experiment":"wolf_diet","merged_sample":{"29a_F260619":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":58,"sample":"29a_F260619","seq_a_single":50,"seq_b_single":50}
ccgc
>HELIUM_000100422_612GNAAXX:7:21:13492:10493#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(A:02)->(C:22)":54,"(G:02)->(C:08)":55},"sample":"15a_F730814","score":1129,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacccgcaat
agcttaaaactcaaatgtcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:49:3435:21131#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"pairing_mismatches":{"(T:31)->(A:09)":21},"sample":"15a_F730814","score":1464,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggccgtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:21:9686:3201#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(a:02)->(g:08)":78,"(a:02)->(t:33)":81,"(a:11)->(c:02)":40,"(a:23)->(c:20)":63,"(c:10)->(g:02)":42,"(t:22)->(a:02)":35},"sample":"29a_F260619","score":263,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacacgtaattaatataacaaaattattagccagagtactaccggcaat
agattaaaactcaaagggcttggcggtgctttataacctt
>HELIUM_000100422_612GNAAXX:7:112:11765:16276#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(C:02)->(G:25)":56,"(G:02)->(C:10)":55},"sample":"29a_F260619","score":1092,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattacacaaacaaaattgttcaccagagtactagccgcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:88:11191:6570#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(T:30)->(A:02)":21},"sample":"26a_F040644","score":2417,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
tcagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatcccc
>HELIUM_000100422_612GNAAXX:7:94:12483:11518#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(A:25)->(G:09)":52,"(C:02)->(G:34)":56,"(C:02)->(T:34)":65},"sample":"29a_F260619","score":1514,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
tcagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:43:12812:12581#0/1_sub[28..127] {"ali_dir":"left","count":10,"experiment":"wolf_diet","merged_sample":{"15a_F730814":5,"29a_F260619":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcctaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:1719:1663#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(A:02)->(G:05)":52,"(C:02)->(A:34)":68,"(C:02)->(G:05)":57,"(T:29)->(G:02)":21},"sample":"13a_F730603","score":1084,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactgccgggaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:114:12721:13724#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":7,"experiment":"wolf_diet","merged_sample":{"26a_F040644":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
acctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:100:8022:19461#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"29a_F260619":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaataattcgccagagaactactagcaac
agattaaacctcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:28:17126:20455#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattagccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:85:3225:11546#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"sample":"26a_F040644","score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatcccc
>HELIUM_000100422_612GNAAXX:7:118:19401:18718#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(a:02)->(g:34)":77,"(a:15)->(c:02)":43,"(c:02)->(a:34)":67,"(c:08)->(a:02)":34},"sample":"13a_F730603","score":196,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaacctattcgcaagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:16:1419:7618#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":63,"pairing_fast_score":0.65,"pairing_mismatches":{"(a:13)->(c:02)":63,"(a:33)->(g:02)":33,"(a:33)->(t:02)":30,"(c:02)->(t:32)":80,"(c:20)->(a:23)":67,"(t:02)->(g:33)":76},"sample":"26a_F040644","score":242,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcatgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:105:6193:6555#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaattttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:113:14054:19223#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(A:32)->(G:07)":37,"(C:02)->(G:16)":42,"(C:02)->(T:34)":65,"(G:02)->(T:09)":56},"sample":"15a_F730814","score":778,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcggcagagtactaccgtcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:102:18107:21114#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaagcacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:118:12806:9177#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(C:02)->(T:32)":65,"(T:32)->(G:11)":38},"sample":"29a_F260619","score":824,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:93:6210:18336#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(C:02)->(A:15)":68,"(C:02)->(T:27)":65,"(T:20)->(G:31)":35},"sample":"29a_F260619","score":1269,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtattcgccagagtactaccggcaat
agcttaaaactcaaagggcttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:59:2390:15297#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaaggatgttcgcaagagtactactagcaatg
gcctaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:111:12261:8185#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(A:02)->(C:11)":54,"(A:02)->(T:09)":52,"(G:02)->(T:05)":55,"(G:02)->(T:08)":62},"sample":"15a_F730814","score":715,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtacttcctgcgat
atcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:57:15349:1745#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(t:33)->(a:02)":35,"(t:34)->(c:02)":38},"sample":"15a_F730814","score":2125,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaacacaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:4:1915:14821#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":33,"pairing_fast_overlap":62,"pairing_fast_score":0.559,"pairing_mismatches":{"(C:02)->(A:32)":59,"(C:02)->(T:34)":65,"(T:02)->(G:13)":35,"(T:34)->(A:06)":24},"sample":"29a_F260619","score":609,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacacgtaattattataacaaaagtattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:68:16342:20480#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"pairing_mismatches":{},"sample":"26a_F040644","score":2212,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:4:13744:13644#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(C:02)->(G:18)":54,"(T:34)->(A:10)":21},"sample":"29a_F260619","score":736,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatgcgccagagtactacgggcaat
agcttaaaactgaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:51:7936:5954#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(A:02)->(C:25)":54,"(A:02)->(G:07)":52},"sample":"15a_F730814","score":1639,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat
agcttaaaacccaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:94:11940:7180#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":63,"pairing_fast_score":0.683,"pairing_mismatches":{"(a:02)->(g:06)":35,"(c:02)->(a:32)":60,"(c:02)->(g:34)":44,"(t:02)->(a:06)":37},"sample":"29a_F260619","score":224,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaatgaatcgccagagaactactagcaaca
gattaaacctcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:52:16144:1280#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{"(c:02)->(t:32)":80},"sample":"26a_F040644","score":2563,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacgacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:37:12457:17677#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":6,"experiment":"wolf_diet","merged_sample":{"26a_F040644":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ctagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":63,"pairing_fast_score":0.583,"pairing_mismatches":{"(A:02)->(G:16)":48,"(A:02)->(T:19)":47,"(C:02)->(A:06)":49,"(C:02)->(A:09)":58,"(G:31)->(A:07)":36,"(T:27)->(A:02)":21,"(T:31)->(G:23)":35},"sample":"26a_F040644","score":693,"score_norm":0.857,"seq_a_single":45,"seq_ab_match":54,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgatactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:95:3505:4629#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":63,"pairing_fast_score":0.75,"pairing_mismatches":{"(A:34)->(C:02)":27,"(C:34)->(A:02)":29,"(G:33)->(A:02)":36,"(T:34)->(G:02)":35},"sample":"26a_F040644","score":1300,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatcgct
>HELIUM_000100422_612GNAAXX:7:16:5254:7363#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(C:10)->(G:30)":56,"(C:29)->(G:09)":53},"sample":"29a_F260619","score":1810,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:112:11113:8862#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:02)->(G:02)":34,"(A:18)->(C:02)":27,"(C:25)->(G:02)":26},"sample":"13a_F730603","score":5,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaarctattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:76:12842:20726#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":11,"experiment":"wolf_diet","merged_sample":{"26a_F040644":11},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagtcctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:23:19216:7014#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctggaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:3:2075:4600#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":589,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttattccctt
>HELIUM_000100422_612GNAAXX:7:2:11720:19103#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagacctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:31:3774:8662#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagcgctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:47:10403:7548#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(C:02)->(A:33)":59},"sample":"13a_F730603","score":1633,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccggagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:7896:18893#0/1_sub[28..128] {"ali_dir":"left","ali_length":61,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":61,"pairing_fast_score":0.914,"pairing_mismatches":{"(g:06)->(a:23)":49},"sample":"13a_F730603","score":1693,"score_norm":0.967,"seq_a_single":47,"seq_ab_match":59,"seq_b_single":47}
ctagccttaaacacaaatagttatgcaaacaaaattgttcgccagagtactagcggcaac
agcttaaaactcaaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:118:17902:2885#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(A:02)->(C:07)":49,"(T:02)->(G:13)":51,"(T:02)->(G:26)":56},"sample":"29a_F260619","score":1071,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtccgaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:111:7400:14616#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttacaccctt
>HELIUM_000100422_612GNAAXX:7:99:17697:3055#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(a:08)->(c:02)":43,"(a:29)->(c:34)":58,"(t:28)->(c:02)":51,"(t:33)->(g:02)":39},"sample":"29a_F260619","score":1586,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgcaagaacactacgagccac
agcttaaaactcaaaggacctggcggtgcttcatatccct
>HELIUM_000100422_612GNAAXX:7:34:8821:4869#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(A:02)->(G:14)":58,"(T:02)->(G:09)":60,"(T:34)->(A:02)":27},"sample":"15a_F730814","score":1143,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcgag
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:28:19415:1906#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(c:02)->(g:02)":56,"(c:24)->(a:02)":42},"sample":"15a_F730814","score":9,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccgscaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:110:19493:7450#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":21,"pairing_fast_overlap":63,"pairing_fast_score":0.35,"pairing_mismatches":{"(A:06)->(C:02)":42,"(A:10)->(G:02)":54,"(A:29)->(G:02)":30,"(A:34)->(G:02)":19,"(C:02)->(A:20)":67,"(C:21)->(A:31)":58,"(C:22)->(G:18)":62,"(G:10)->(C:15)":63,"(T:18)->(G:02)":50,"(T:27)->(C:02)":37},"sample":"26a_F040644","score":55,"score_norm":0.81,"seq_a_single":45,"seq_ab_match":51,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaaca
gcctgaaactcaaagggcttggcggttctttacatccct
>HELIUM_000100422_612GNAAXX:7:5:12092:12350#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":63,"pairing_fast_score":0.75,"pairing_mismatches":{"(a:13)->(t:02)":47,"(c:02)->(t:34)":80,"(t:06)->(c:02)":52},"sample":"26a_F040644","score":892,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacatacacattcaataaacaagaatgttcgccagagaactattagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:28:19349:7063#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":32,"pairing_fast_overlap":62,"pairing_fast_score":0.542,"pairing_mismatches":{"(A:02)->(T:06)":58,"(A:07)->(G:24)":44,"(A:30)->(C:10)":52,"(A:34)->(T:02)":28,"(G:34)->(A:02)":20,"(T:09)->(G:02)":22,"(T:23)->(G:07)":39},"sample":"13a_F730603","score":655,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccggagtactaccggctat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:72:18993:4004#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(c:02)->(a:34)":74,"(c:06)->(a:02)":37,"(c:07)->(t:02)":48,"(c:17)->(a:02)":42},"sample":"29a_F260619","score":70,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattcttcgccagagcactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:28:2216:4288#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(C:02)->(G:14)":57},"sample":"13a_F730603","score":1933,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccgggaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:73:1655:15092#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":63,"pairing_fast_score":0.917,"pairing_mismatches":{},"sample":"26a_F040644","score":3287,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataagctattccataacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:117:17406:9317#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":63,"pairing_fast_score":0.65,"pairing_mismatches":{"(A:02)->(C:34)":56,"(T:02)->(G:34)":61,"(T:32)->(A:02)":21},"sample":"29a_F260619","score":1343,"score_norm":0.841,"seq_a_single":45,"seq_ab_match":53,"seq_b_single":45}
ttagccctaaacatacgctattccataacaaaataattcgccagagaactactagcaaca
gattaaacctcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:113:12401:16698#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":63,"pairing_fast_score":0.667,"pairing_mismatches":{"(A:02)->(T:34)":64,"(C:02)->(A:32)":58,"(C:23)->(A:17)":28},"sample":"26a_F040644","score":1348,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacatagataattttacaccaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:115:13728:16624#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(c:07)->(g:02)":42,"(t:02)->(g:08)":80,"(t:10)->(a:02)":46},"sample":"15a_F730814","score":999,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagtgtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:94:8976:10002#0/1_sub[28..127] {"ali_dir":"left","count":5,"experiment":"wolf_diet","merged_sample":{"29a_F260619":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"sample":"29a_F260619"}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttgacggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:104:10267:11548#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":29,"pairing_fast_overlap":62,"pairing_fast_score":0.492,"pairing_mismatches":{"(a:10)->(c:02)":43,"(a:28)->(t:02)":33,"(c:02)->(t:19)":81,"(c:06)->(a:02)":46,"(c:21)->(t:02)":48,"(c:29)->(a:32)":75,"(t:23)->(g:02)":71},"sample":"15a_F730814","score":27,"score_norm":0.839,"seq_a_single":46,"seq_ab_match":52,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgcaagcgcactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:51:17179:21301#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(C:02)->(T:34)":65,"(C:34)->(A:02)":30,"(T:29)->(A:02)":35,"(T:34)->(A:02)":21},"sample":"15a_F730814","score":682,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttgacggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:55:18513:15799#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatggttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:99:19068:1147#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":33,"pairing_fast_overlap":62,"pairing_fast_score":0.559,"pairing_mismatches":{"(A:02)->(G:11)":68,"(A:29)->(G:02)":24,"(C:02)->(T:25)":65,"(T:09)->(G:19)":39,"(T:27)->(A:02)":21,"(T:28)->(G:18)":36},"sample":"29a_F260619","score":1202,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatgcgccagagtactaccggcaat
agcttaagactcaaaggacttggcggttctttataccctt
>HELIUM_000100422_612GNAAXX:7:116:4500:14618#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":63,"pairing_fast_score":0.683,"pairing_mismatches":{"(A:22)->(T:07)":51,"(A:26)->(G:06)":33,"(T:25)->(G:06)":35,"(T:34)->(G:02)":21},"sample":"26a_F040644","score":1909,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaagggcgtggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:17:2707:11559#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":62,"pairing_fast_score":0.593,"pairing_mismatches":{"(A:02)->(G:23)":37,"(A:02)->(G:30)":52,"(C:02)->(G:34)":56,"(T:30)->(A:08)":25},"sample":"29a_F260619","score":626,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattgttcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:98:10839:20244#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaaggatgttcgccagagtactactagcaatg
gcctaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:56:17644:11356#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"pairing_mismatches":{},"sample":"26a_F040644","score":3997,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacatggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:113:4543:16579#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(c:02)->(a:31)":67,"(t:31)->(a:02)":35},"sample":"29a_F260619","score":669,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccataaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:84:13238:8422#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:32)->(G:11)":44,"(C:02)->(G:25)":53},"sample":"29a_F260619","score":1010,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
tcagccctaaacacaagtaattaatataacaaaattattcgccagagtactagcggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:62:8682:3502#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{},"sample":"26a_F040644","score":709,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
tcagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:86:11111:5480#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(c:18)->(a:29)":42},"sample":"15a_F730814","score":2084,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgacagagtactaccggcaat
agcttaaaacccaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:79:5035:1949#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(A:06)->(T:02)":51,"(C:12)->(G:02)":56,"(C:34)->(A:02)":30,"(T:02)->(C:28)":63,"(T:25)->(G:02)":35},"sample":"29a_F260619","score":76,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
tcagccctaaacacaagtaattaatataacaaaattattcgccagagtacaaccgccaat
agctaaaaactcaaaggactgggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:11:12154:19712#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(A:02)->(C:22)":54,"(A:02)->(T:34)":65,"(T:15)->(G:26)":36,"(T:28)->(G:14)":21},"sample":"29a_F260619","score":1345,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaatgattcgccagagtactaccggcaat
agcttaaaactcaaagggcttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:15491:6252#0/1_sub[28..126] {"ali_dir":"left","count":6,"experiment":"wolf_diet","merged_sample":{"26a_F040644":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtattactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:60:3552:11533#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":30,"experiment":"wolf_diet","merged_sample":{"15a_F730814":24,"29a_F260619":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagggtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:74:15104:10119#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"13a_F730603":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctctataccctt
>HELIUM_000100422_612GNAAXX:7:100:13730:16676#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(A:02)->(C:34)":65},"sample":"13a_F730603","score":1641,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattatataaacaaaattattcgccagagtactaccggcaac
agcccaaaactcaaaggacttggcggtgcttcataccctt
>HELIUM_000100422_612GNAAXX:7:115:18481:9138#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":32,"pairing_fast_overlap":62,"pairing_fast_score":0.542,"pairing_mismatches":{"(C:02)->(A:34)":68,"(C:02)->(G:34)":56,"(C:02)->(T:32)":65,"(G:02)->(T:08)":41,"(T:02)->(G:10)":35},"sample":"15a_F730814","score":555,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtattctccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:13:19007:21225#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(a:02)->(c:34)":72,"(a:08)->(c:33)":57,"(a:16)->(c:02)":43,"(c:02)->(a:33)":59,"(c:02)->(g:34)":77,"(c:02)->(t:33)":71,"(c:15)->(g:30)":56,"(t:02)->(c:34)":70},"sample":"29a_F260619","score":642,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ctagccctaaacacaagtaattaatataacaaaattattcgcaagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:34:13086:6440#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":14,"experiment":"wolf_diet","merged_sample":{"13a_F730603":14},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaaataattatataaacaaaattattcgccagagtactaccggcaac
agcccaaaactcaaaggacttggcggtgcttcacaccctt
>HELIUM_000100422_612GNAAXX:7:79:18836:12604#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(A:22)->(G:02)":27,"(A:34)->(G:02)":31,"(G:34)->(A:02)":20,"(T:02)->(G:07)":71},"sample":"13a_F730603","score":602,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaacgcaaaggactgggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:56:19543:8102#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtactttataccctt
>HELIUM_000100422_612GNAAXX:7:7:6056:11975#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"13a_F730603":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtacttccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:86:15486:9039#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(c:13)->(a:33)":73,"(t:34)->(g:10)":37},"sample":"26a_F040644","score":3584,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccttaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:48:2569:3252#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctcaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:36:17032:18995#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(c:26)->(a:31)":68},"sample":"15a_F730814","score":3643,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccggagtactaccggcaat
agcttaaaactcaaaggacttggcggtgcttcataccctt
>HELIUM_000100422_612GNAAXX:7:7:18958:1204#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(t:09)->(c:02)":49,"(t:33)->(c:02)":37},"sample":"26a_F040644","score":1443,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggattactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:51:15825:6498#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"pairing_mismatches":{"(a:02)->(t:32)":81,"(c:02)->(a:32)":67},"sample":"13a_F730603","score":2265,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaataagtatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:38:18188:17945#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(c:02)->(a:27)":78,"(c:02)->(t:34)":81},"sample":"29a_F260619","score":1494,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcagaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:14:17587:5118#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttagaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:6:17526:4815#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(a:02)->(g:34)":77},"sample":"13a_F730603","score":2438,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaataattcgccagaggactactagcaat
agcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:22:1359:6445#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(a:06)->(t:02)":48,"(c:07)->(t:02)":39,"(c:10)->(g:02)":56,"(c:11)->(g:33)":77},"sample":"29a_F260619","score":1078,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattatccgccagagaactaccgccaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:95:5892:14633#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":25,"pairing_fast_overlap":62,"pairing_fast_score":0.424,"pairing_mismatches":{"(a:02)->(c:28)":43,"(a:02)->(c:34)":72,"(a:02)->(g:34)":77,"(a:02)->(t:24)":48,"(a:02)->(t:34)":65,"(c:02)->(g:06)":42,"(c:02)->(g:25)":56,"(c:02)->(t:26)":51,"(g:02)->(a:08)":55,"(g:02)->(t:34)":81},"sample":"15a_F730814","score":655,"score_norm":0.823,"seq_a_single":46,"seq_ab_match":51,"seq_b_single":46}
ttagccctaaacacaagcaattaatataacaaaattattcggcagagtactaccagcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:114:4769:20092#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:02)->(G:09)":49,"(A:02)->(G:21)":69,"(A:32)->(G:02)":23,"(T:34)->(G:02)":21},"sample":"15a_F730814","score":771,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat
agcttaaagctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:118:17777:11760#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(T:34)->(G:24)":25},"sample":"15a_F730814","score":1267,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagcccttaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:82:14206:15498#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"sample":"29a_F260619","score":1235,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagtcctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:99:14093:16138#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccgggaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:118:13448:19283#0/1_sub[26..124] {"ali_dir":"left","ali_length":65,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":65,"pairing_fast_score":0.694,"pairing_mismatches":{"(a:02)->(g:32)":76,"(a:07)->(c:02)":56,"(a:10)->(c:02)":41,"(t:07)->(c:02)":49},"sample":"26a_F040644","score":995,"score_norm":0.923,"seq_a_single":43,"seq_ab_match":60,"seq_b_single":43}
ttagccctaaacatggataattttacaacaaaataattcgacagaggattactagaaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:96:16311:15313#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":63,"pairing_fast_score":0.75,"pairing_mismatches":{"(A:02)->(G:11)":67,"(C:34)->(A:02)":23,"(T:33)->(A:02)":21},"sample":"29a_F260619","score":1927,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactactagcaaca
gattaagcctcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:35:12666:1702#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":9,"experiment":"wolf_diet","merged_sample":{"15a_F730814":5,"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactcccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:113:9632:20233#0/1_sub[28..127] {"ali_dir":"left","count":21,"experiment":"wolf_diet","merged_sample":{"15a_F730814":15,"29a_F260619":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcgat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:79:14346:17777#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgctactagcaaca
tcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:92:17238:18966#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":18,"experiment":"wolf_diet","merged_sample":{"26a_F040644":18},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagcactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:106:3031:14497#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":8,"experiment":"wolf_diet","merged_sample":{"13a_F730603":8},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgcaagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:72:18286:18633#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":27,"experiment":"wolf_diet","merged_sample":{"15a_F730814":12,"29a_F260619":15},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggactgggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:74:2977:10801#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(a:02)->(t:12)":81,"(a:33)->(g:02)":32,"(c:07)->(t:02)":36,"(c:22)->(t:02)":48},"sample":"29a_F260619","score":202,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaatcattcgccagagcactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:38:12579:14367#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgcaagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:80:7033:10034#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"pairing_mismatches":{"(t:31)->(g:10)":39,"(t:33)->(c:10)":38},"sample":"15a_F730814","score":3193,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccgccaat
agcttaaaactcaaaggacctggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:41:6251:9380#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":63,"pairing_fast_score":0.717,"pairing_mismatches":{"(A:02)->(G:14)":51,"(T:02)->(G:19)":70,"(T:34)->(A:02)":21},"sample":"26a_F040644","score":608,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactgctagcaaca
gcctgaaacgcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:89:19560:4207#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(A:32)->(C:06)":34,"(G:02)->(T:07)":65},"sample":"26a_F040644","score":1814,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatattcgccagagtactactagcaaca
gccttaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:12:11171:17487#0/1_sub[28..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":61,"pairing_fast_overlap":64,"pairing_fast_score":1,"sample":"26a_F040644","score":4203,"score_norm":1,"seq_a_single":44,"seq_ab_match":64,"seq_b_single":44}
tagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacag
cctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:2:11902:5892#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"pairing_mismatches":{"(A:34)->(T:02)":19,"(G:33)->(A:02)":20},"sample":"13a_F730603","score":3247,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccgcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:92:18729:1707#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatctaacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:80:3182:11111#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggtggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:49:2538:18645#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(A:02)->(G:09)":66,"(T:34)->(G:02)":20},"sample":"26a_F040644","score":1228,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctggaactcgaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:72:2783:12920#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacactcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:4:18969:19813#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaaataattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:73:10944:14101#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaaataattatataaacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:61:17561:21218#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaatcattctataacaaaataattcgccggagaactactaggaaca
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:22:15279:13531#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":28,"pairing_fast_overlap":62,"pairing_fast_score":0.475,"pairing_mismatches":{"(a:34)->(g:02)":49,"(c:33)->(g:02)":42,"(c:34)->(g:02)":57,"(t:02)->(g:15)":80,"(t:30)->(g:02)":36,"(t:30)->(g:23)":71,"(t:33)->(a:02)":38},"sample":"15a_F730814","score":351,"score_norm":0.839,"seq_a_single":46,"seq_ab_match":52,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacgtggcggtgctttattgcctt
>HELIUM_000100422_612GNAAXX:7:111:17629:12480#0/1_sub[28..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":64,"pairing_fast_score":0.869,"pairing_mismatches":{"(c:32)->(g:02)":40},"sample":"26a_F040644","score":773,"score_norm":0.969,"seq_a_single":44,"seq_ab_match":62,"seq_b_single":44}
ttagccctaaacatagatattttacaacaaaataattcgccagaggactactagcaatag
cttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:7:4341:3843#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(a:34)->(g:09)":33},"sample":"26a_F040644","score":3428,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctaaaactcaaaggacttggcggtactttatatccct
>HELIUM_000100422_612GNAAXX:7:104:13669:16989#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(C:02)->(A:13)":59,"(C:02)->(A:32)":67,"(C:07)->(A:02)":24,"(G:02)->(A:06)":55,"(T:02)->(G:25)":56},"sample":"15a_F730814","score":397,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattactataacaaaattattcgccagagtactaccagcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:33:5095:21060#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(C:02)->(A:31)":59,"(C:02)->(T:34)":65,"(C:23)->(G:30)":56,"(T:02)->(G:10)":64,"(T:02)->(G:23)":71},"sample":"29a_F260619","score":1368,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcgtaaaacgcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:27:14107:10052#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"pairing_mismatches":{"(T:34)->(A:08)":21},"sample":"29a_F260619","score":1860,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:104:3135:20184#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":63,"pairing_fast_score":0.717,"pairing_mismatches":{"(a:02)->(c:34)":69,"(c:02)->(a:34)":68,"(c:02)->(t:33)":80,"(c:21)->(t:02)":47,"(c:31)->(a:02)":41},"sample":"26a_F040644","score":330,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagcactactagcaaca
gcctgaaactcaaaggacttggcggtgccttacatccct
>HELIUM_000100422_612GNAAXX:7:14:19295:13446#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":33,"pairing_fast_overlap":63,"pairing_fast_score":0.55,"pairing_mismatches":{"(A:02)->(G:12)":51,"(A:30)->(G:02)":33,"(C:02)->(A:32)":67,"(C:31)->(G:02)":22,"(G:25)->(T:02)":32,"(G:30)->(T:09)":44,"(T:29)->(G:15)":38},"sample":"26a_F040644","score":387,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactgcttgcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:19:4737:2252#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttagaccctt
>HELIUM_000100422_612GNAAXX:7:48:19024:12336#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(A:02)->(G:09)":49,"(A:02)->(G:14)":67,"(T:34)->(A:02)":25,"(T:34)->(G:18)":38},"sample":"15a_F730814","score":745,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat
agcttagaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:75:13072:20644#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":10,"experiment":"wolf_diet","merged_sample":{"15a_F730814":10},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacgagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:43:7893:3974#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:25)->(T:25)":49,"(A:29)->(T:13)":58,"(C:28)->(G:07)":57},"sample":"29a_F260619","score":2102,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtwctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:40:10055:18093#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(C:25)":54,"(A:02)->(G:09)":49,"(A:02)->(G:13)":69},"sample":"15a_F730814","score":1145,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatatagcaaaattattcgccagagtgctaccggcaat
agcttaaagctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:6:16006:1555#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(c:32)->(a:34)":75,"(t:32)->(g:32)":80},"sample":"29a_F260619","score":2694,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacktggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:117:4889:8651#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(c:11)->(t:02)":47,"(c:22)->(a:14)":68},"sample":"26a_F040644","score":1235,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagcactactagcaaca
gcctgaacctcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:41:18131:7853#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":63,"pairing_fast_score":0.75,"pairing_mismatches":{"(A:32)->(C:02)":49,"(T:32)->(C:02)":39,"(T:34)->(A:02)":25},"sample":"15a_F730814","score":640,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggactggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:10:10460:13805#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:02)->(T:34)":65,"(C:02)->(T:31)":39,"(T:24)->(G:24)":35},"sample":"15a_F730814","score":651,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaaktattcgccagagtactaccggcaat
agcttaaaactcaaaggactgggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:89:3182:1995#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":63,"pairing_fast_score":0.717,"pairing_mismatches":{"(A:09)->(C:09)":58,"(A:12)->(G:20)":54,"(A:34)->(C:02)":19,"(A:34)->(G:02)":30,"(T:33)->(G:02)":38},"sample":"26a_F040644","score":1464,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactggcamca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:102:1867:1082#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactaccaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:7:9420:4204#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":8,"experiment":"wolf_diet","merged_sample":{"26a_F040644":8},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtacgactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:41:15835:16770#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"26a_F040644","score":3531,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagagaactactagcaaca
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:118:6316:21098#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(A:02)->(T:09)":52,"(C:02)->(A:34)":66,"(T:34)->(G:02)":27},"sample":"15a_F730814","score":356,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtacttctggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:49:4681:16126#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"13a_F730603","score":2941,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttgtgatgctttataccctt
>HELIUM_000100422_612GNAAXX:7:78:1786:10506#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":63,"pairing_fast_score":0.883,"pairing_mismatches":{"(T:28)->(G:12)":53},"sample":"26a_F040644","score":2111,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaata
gcctgaaactcaaaggacgtggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:106:8696:19513#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":63,"pairing_fast_score":0.817,"pairing_mismatches":{"(c:02)->(a:34)":74,"(c:02)->(g:34)":76},"sample":"26a_F040644","score":2822,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactactagcaaca
gattaaacctcaaaggacttggcagtgctttacacccct
>HELIUM_000100422_612GNAAXX:7:34:15738:4797#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":10,"experiment":"wolf_diet","merged_sample":{"15a_F730814":6,"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctctataccctt
>HELIUM_000100422_612GNAAXX:7:94:6384:20392#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":30,"experiment":"wolf_diet","merged_sample":{"15a_F730814":22,"29a_F260619":8},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:87:9478:19196#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":7,"experiment":"wolf_diet","merged_sample":{"26a_F040644":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatattcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:108:1608:1208#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:34)->(C:05)":28,"(T:21)->(G:15)":39,"(T:31)->(G:20)":21},"sample":"13a_F730603","score":1169,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgcttgataccctt
>HELIUM_000100422_612GNAAXX:7:100:10697:9739#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":8,"experiment":"wolf_diet","merged_sample":{"26a_F040644":8},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaacttaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:89:5408:10080#0/1_sub[28..127] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":64,"pairing_fast_score":0.869,"pairing_mismatches":{"(C:30)->(T:02)":22},"sample":"13a_F730603","score":1742,"score_norm":0.969,"seq_a_single":44,"seq_ab_match":62,"seq_b_single":44}
ctagccttaaacacaaatagtcatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:96:2560:17067#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"pairing_mismatches":{},"sample":"26a_F040644","score":2184,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccggagaactactagcaaca
gcttaaaactcaaaggacttggcggtgccttacgtccct
>HELIUM_000100422_612GNAAXX:7:100:13324:4922#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"26a_F040644","score":3556,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagctctaaacataaacattcagtaaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:57:3104:11078#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(C:02)->(A:34)":59},"sample":"15a_F730814","score":1625,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agtttaaaactcaaaggacctggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:81:18677:10557#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:02)->(G:13)":69,"(A:02)->(G:15)":58},"sample":"13a_F730603","score":730,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcgat
agcttaaagctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:117:19054:11242#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(a:06)->(t:02)":39,"(c:02)->(a:34)":75,"(c:02)->(t:34)":81,"(c:26)->(t:32)":65,"(t:10)->(a:02)":35},"sample":"15a_F730814","score":712,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctacacacaagtaattaatataacaaaattatacgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:93:7569:17305#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaatcagtctataacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:76:7101:16258#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(T:33)->(G:27)":37},"sample":"26a_F040644","score":2948,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataagctattctataacaaaataattcgccagagaactactagcaaca
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:116:16321:17213#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(A:02)->(C:21)":54,"(A:02)->(G:19)":52,"(C:02)->(A:30)":59,"(C:02)->(T:34)":65,"(G:02)->(T:08)":62},"sample":"29a_F260619","score":1067,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat
atcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:87:12797:14333#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(c:18)->(a:02)":59},"sample":"15a_F730814","score":39,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcact
agcttaaaactcaaaggacttggcggtgctttattccctt
>HELIUM_000100422_612GNAAXX:7:38:11381:7702#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(a:02)->(c:27)":72,"(c:09)->(g:29)":56,"(t:25)->(g:02)":38},"sample":"29a_F260619","score":1321,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaatcacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:83:10811:5080#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{},"sample":"15a_F730814","score":2487,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgcattataccctt
>HELIUM_000100422_612GNAAXX:7:77:7022:10480#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":1959,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaaggatgttcgcaagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:116:11906:5624#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(A:02)->(T:07)":58,"(A:02)->(T:09)":52,"(A:34)->(T:08)":32,"(C:02)->(G:10)":56,"(T:26)->(A:09)":35},"sample":"15a_F730814","score":976,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtacttccggctat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:19:16235:10173#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(A:02)->(G:13)":69},"sample":"13a_F730603","score":2163,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaagctcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:2:2250:10182#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(c:02)->(t:21)":72},"sample":"13a_F730603","score":1488,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaacttaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:70:9514:21133#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"15a_F730814","score":4156,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacacaagtaattaatataacaaattattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:87:18699:11133#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:28)->(G:02)":31,"(A:31)->(G:02)":33,"(C:10)->(G:32)":56,"(T:31)->(G:02)":22,"(T:33)->(A:02)":27},"sample":"15a_F730814","score":724,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggactgggcggtgctttatgccctc
>HELIUM_000100422_612GNAAXX:7:52:9872:16545#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(G:02)->(T:08)":55},"sample":"29a_F260619","score":1340,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaat
agcttaaaactcaaaggacttggcggtgccttataccctt
>HELIUM_000100422_612GNAAXX:7:115:4014:12109#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(C:02)->(A:31)":59,"(C:02)->(G:33)":56,"(C:02)->(T:34)":65,"(G:02)->(A:27)":55,"(T:02)->(G:21)":38},"sample":"13a_F730603","score":118,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactagtcgccagagtactaccagcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:55:11954:15731#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":6,"experiment":"wolf_diet","merged_sample":{"29a_F260619":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"29a_F260619","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactactagcaaca
gattaaacctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:112:12202:9452#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(T:02)->(A:06)":70},"sample":"26a_F040644","score":437,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaacacaaaggtcttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:109:2329:17350#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":11,"experiment":"wolf_diet","merged_sample":{"15a_F730814":8,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgcttcataccctt
>HELIUM_000100422_612GNAAXX:7:84:15029:16032#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":10,"experiment":"wolf_diet","merged_sample":{"15a_F730814":9,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactagcggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:4:12119:20156#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":9,"experiment":"wolf_diet","merged_sample":{"26a_F040644":9},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacgtccct
>HELIUM_000100422_612GNAAXX:7:3:15622:13794#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":6,"experiment":"wolf_diet","merged_sample":{"13a_F730603":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagttctaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:90:5952:4042#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(a:06)->(t:06)":44},"sample":"13a_F730603","score":3227,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccwgagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:45:9786:20570#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":63,"pairing_fast_score":0.6,"pairing_mismatches":{"(A:15)->(G:02)":54,"(A:31)->(G:02)":48,"(C:09)->(A:02)":58,"(T:27)->(G:02)":38,"(T:34)->(A:02)":25},"sample":"26a_F040644","score":822,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcacca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:105:16063:5042#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3807,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagtcctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:2:7369:20864#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":27,"pairing_fast_overlap":62,"pairing_fast_score":0.458,"pairing_mismatches":{"(A:32)->(G:02)":52,"(A:34)->(C:02)":28,"(C:30)->(A:02)":40,"(T:21)->(G:02)":48,"(T:22)->(G:02)":36,"(T:31)->(G:02)":22,"(T:34)->(G:02)":27},"sample":"29a_F260619","score":254,"score_norm":0.839,"seq_a_single":46,"seq_ab_match":52,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaagggcttggcggtgctttataccctg
>HELIUM_000100422_612GNAAXX:7:118:4007:15400#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(A:34)->(G:02)":27,"(C:02)->(G:02)":41},"sample":"26a_F040644","score":1,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgscagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:85:10933:3201#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"pairing_mismatches":{},"sample":"15a_F730814","score":1596,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:32:14758:12239#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(t:31)->(g:09)":37},"sample":"26a_F040644","score":3770,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaagaatgttcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:20:12998:21152#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":62,"pairing_fast_score":0.712,"pairing_mismatches":{"(a:15)->(c:08)":53,"(c:13)->(a:34)":68,"(c:23)->(a:34)":74,"(c:27)->(t:34)":81,"(t:12)->(g:11)":39},"sample":"29a_F260619","score":2814,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaacggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:4:9725:19937#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacctaaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:37:7300:15013#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:02)->(G:08)":49},"sample":"15a_F730814","score":1339,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat
aacttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:12:14070:8299#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(t:29)->(c:02)":37},"sample":"26a_F040644","score":1686,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataatttcacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:106:18483:14251#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":63,"pairing_fast_score":0.817,"pairing_mismatches":{"(C:34)->(G:02)":49},"sample":"29a_F260619","score":802,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactactagcaaca
gattaaacctcaaaggacttggcagtgctttataccccg
>HELIUM_000100422_612GNAAXX:7:83:14194:15323#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":63,"pairing_fast_score":0.817,"pairing_mismatches":{"(A:02)->(G:30)":45},"sample":"26a_F040644","score":1255,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccaggggactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:7:13939:11159#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(A:02)->(T:05)":51},"sample":"26a_F040644","score":1415,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtacttctagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:69:13624:18920#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(C:31)->(G:02)":30,"(T:34)->(G:02)":38},"sample":"13a_F730603","score":320,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggccttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:84:14502:1617#0/1_sub[28..127] {"ali_dir":"left","count":319,"experiment":"wolf_diet","merged_sample":{"29a_F260619":319},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"sample":"29a_F260619"}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:8404:14511#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":12,"experiment":"wolf_diet","merged_sample":{"26a_F040644":12},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:54:15067:12524#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":26,"experiment":"wolf_diet","merged_sample":{"26a_F040644":26},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:2:5998:1617#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":12,"experiment":"wolf_diet","merged_sample":{"26a_F040644":12},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcgatgctttacatccct
>HELIUM_000100422_612GNAAXX:7:80:19721:5420#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":13,"experiment":"wolf_diet","merged_sample":{"26a_F040644":13},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgctcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:77:1849:12593#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"13a_F730603","seq_a_single":45,"seq_b_single":45}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:77:11457:21064#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(C:02)->(G:28)":56,"(T:02)->(G:14)":51},"sample":"15a_F730814","score":356,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtacggccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:78:6237:19248#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(A:02)->(G:20)":59,"(A:02)->(G:28)":52,"(C:14)->(G:02)":42,"(T:14)->(A:02)":24},"sample":"13a_F730603","score":249,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactgccggcagt
agcttaaaactcaaagggcttggcggtgctgtatgccctt
>HELIUM_000100422_612GNAAXX:7:52:15545:10312#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"sample":"29a_F260619","score":2928,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaaataattacgcaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:2845:14400#0/1_sub[27..124] {"ali_dir":"left","ali_length":65,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":65,"pairing_fast_score":0.774,"pairing_mismatches":{"(A:02)->(T:07)":50,"(A:34)->(G:02)":19,"(G:02)->(A:24)":44,"(G:02)->(T:24)":36},"sample":"26a_F040644","score":248,"score_norm":0.938,"seq_a_single":43,"seq_ab_match":61,"seq_b_single":43}
ttagccctaaacataaacattcaataaacagaatgttcgccagagtacttctagcaacag
cctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:63:9712:21272#0/1_sub[79..82] {"ali_dir":"right","count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_mismatches":{},"sample":"29a_F260619"}
cttt
>HELIUM_000100422_612GNAAXX:7:114:13189:1947#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(T:02)->(G:02)":50},"sample":"26a_F040644","score":7,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtackactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:109:11470:19745#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(a:07)->(c:02)":40,"(c:02)->(a:34)":67,"(c:02)->(g:23)":42},"sample":"15a_F730814","score":51,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattaggcagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:31:17486:11347#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattgaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:75:3157:4125#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaacgattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:84:8652:21031#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":63,"pairing_fast_score":0.617,"pairing_mismatches":{"(A:24)->(G:17)":48,"(A:34)->(G:02)":27,"(A:34)->(G:18)":34,"(T:28)->(G:06)":53,"(T:32)->(G:02)":20,"(T:32)->(G:32)":38,"(T:34)->(A:02)":21},"sample":"26a_F040644","score":1814,"score_norm":0.889,"seq_a_single":45,"seq_ab_match":56,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtkcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:19:12486:13267#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(c:34)->(a:07)":41},"sample":"26a_F040644","score":3177,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattccccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:100:3664:2896#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(A:02)->(T:24)":39,"(A:02)->(T:31)":65,"(C:02)->(A:31)":59,"(T:02)->(G:26)":36},"sample":"15a_F730814","score":775,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaatgattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:35:19222:9023#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(c:10)->(g:34)":77},"sample":"29a_F260619","score":3467,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaagttattcgccagagtactaccggcaat
agtttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:108:7830:8252#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(G:09)":52,"(C:02)->(A:34)":59,"(T:24)->(G:32)":35},"sample":"29a_F260619","score":1082,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtattcgccagagtactgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:77:17256:20277#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(a:06)->(c:02)":40,"(c:02)->(a:31)":69,"(c:02)->(g:34)":77,"(c:02)->(t:34)":81,"(t:21)->(g:02)":35},"sample":"29a_F260619","score":390,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattactataacaaaattattagccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:71:5591:1755#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":15,"experiment":"wolf_diet","merged_sample":{"15a_F730814":5,"29a_F260619":10},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatatcctt
>HELIUM_000100422_612GNAAXX:7:6:10451:2548#0/1_sub[28..126] {"ali_dir":"left","count":12,"experiment":"wolf_diet","merged_sample":{"26a_F040644":12},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"sample":"26a_F040644"}
ttagccctaaacataaacagtcaataaacaaggatgttcgccagagtactactagcaatg
gcctaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:96:15296:18997#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(A:02)->(G:07)":34,"(A:02)->(G:18)":58,"(C:02)->(A:34)":69,"(C:02)->(G:32)":56,"(T:02)->(G:09)":38},"sample":"15a_F730814","score":680,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaagttagtcgccagagtactaccggcgat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:51:18572:15411#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":6,"experiment":"wolf_diet","merged_sample":{"26a_F040644":6},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttgcatccct
>HELIUM_000100422_612GNAAXX:7:46:3389:1110#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(c:06)->(t:26)":47},"sample":"26a_F040644","score":2894,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactatcaaca
gcctgaaactcaaaggacttggcggtgccttacatccct
>HELIUM_000100422_612GNAAXX:7:91:15639:18238#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":32,"pairing_fast_overlap":63,"pairing_fast_score":0.533,"pairing_mismatches":{"(c:02)->(a:22)":68,"(c:02)->(a:32)":77,"(c:02)->(a:34)":74,"(c:02)->(g:32)":65,"(c:02)->(t:34)":80,"(c:07)->(g:02)":46,"(g:02)->(c:22)":69},"sample":"26a_F040644","score":45,"score_norm":0.857,"seq_a_single":45,"seq_ab_match":54,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagacgactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:90:5930:3388#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(c:20)->(a:02)":33},"sample":"13a_F730603","score":1723,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaacactattcgccagaggactactagcaat
agcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:119:5332:15215#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":63,"pairing_fast_score":0.583,"pairing_mismatches":{"(A:02)->(T:10)":48,"(C:02)->(A:33)":58},"sample":"26a_F040644","score":211,"score_norm":0.873,"seq_a_single":45,"seq_ab_match":55,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagttctactagcaaca
gcctgaaactcaaagggcttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:56:7365:1124#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{"(A:02)->(G:26)":65},"sample":"26a_F040644","score":1160,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcttgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:16:4507:6027#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(a:17)->(c:32)":54},"sample":"15a_F730814","score":2684,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatttaacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:5440:11277#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:02)->(T:05)":52,"(G:02)->(T:20)":55,"(T:02)->(G:16)":51},"sample":"13a_F730603","score":1094,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtacgtcctgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:91:8597:6626#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"sample":"26a_F040644","score":1391,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaagcataaacattcaataaacaagaatattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:26:7298:1457#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":7,"experiment":"wolf_diet","merged_sample":{"26a_F040644":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatggtcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:92:13949:14278#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(c:02)->(a:34)":68,"(c:34)->(a:17)":42,"(g:02)->(t:34)":81},"sample":"13a_F730603","score":2135,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaccacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:23:10872:20213#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaatcattctataacaaaataattcgccggagaactactagcaaca
gcttaaaactcaaaggacttggcggtgccttacgtccct
>HELIUM_000100422_612GNAAXX:7:100:2426:17903#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(A:02)->(C:23)":54,"(A:02)->(G:13)":49,"(T:20)->(G:21)":35},"sample":"29a_F260619","score":794,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtatttgccagagtgctaccggcaat
agcttaaaactcaaaggacttggcgttgctttataccctt
>HELIUM_000100422_612GNAAXX:7:6:11665:19853#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(C:23)->(A:14)":27,"(T:02)->(G:10)":53},"sample":"26a_F040644","score":1756,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaatacacaagaatgttcgccagagtactacgagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:75:15735:17933#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(t:31)->(g:02)":39,"(t:33)->(g:02)":35},"sample":"15a_F730814","score":1299,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctgtatgccctt
>HELIUM_000100422_612GNAAXX:7:50:10637:6527#0/1_sub[28..126] {"ali_dir":"left","count":366,"experiment":"wolf_diet","merged_sample":{"13a_F730603":13,"15a_F730814":5,"26a_F040644":347,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:98:6034:5203#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:72:18044:18151#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"15a_F730814":6,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtgattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:71:7857:4233#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaaktattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:101:12204:8132#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(c:02)->(a:34)":74,"(c:29)->(a:06)":41,"(g:02)->(a:09)":46},"sample":"26a_F040644","score":1211,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaagactactagcaata
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:53:10400:8674#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(t:10)->(c:10)":42},"sample":"29a_F260619","score":2543,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgycagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:76:15028:15520#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:02)->(G:12)":61,"(T:02)->(G:15)":71,"(T:33)->(A:02)":22},"sample":"15a_F730814","score":1454,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
ggcttaaaacgcaaaggacttggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:109:14231:9142#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(C:02)->(A:34)":67,"(C:02)->(G:30)":56,"(G:15)->(A:02)":20},"sample":"29a_F260619","score":1049,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttcgccctaaacacaagtagttaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:28:12048:5011#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":4005,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttaggcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:110:12824:2822#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"13a_F730603":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactatttgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:85:14462:19175#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":29,"pairing_fast_overlap":62,"pairing_fast_score":0.492,"pairing_mismatches":{"(A:02)->(G:12)":52,"(A:02)->(G:18)":61,"(A:30)->(G:15)":37,"(C:02)->(G:30)":56,"(C:27)->(G:02)":26,"(G:34)->(A:02)":20,"(T:30)->(G:02)":21},"sample":"13a_F730603","score":579,"score_norm":0.839,"seq_a_single":46,"seq_ab_match":52,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactgccggcaat
ggcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:3:1568:20543#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"15a_F730814","seq_a_single":45,"seq_b_single":45}
ttagccctaacacaagtaattaatataacaaaattattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:119:8901:20958#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgscagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:13:2954:20745#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":33,"pairing_fast_overlap":62,"pairing_fast_score":0.559,"pairing_mismatches":{"(A:27)->(C:02)":34,"(A:28)->(G:10)":49,"(A:33)->(T:19)":58,"(C:18)->(G:02)":26,"(C:26)->(G:02)":43,"(C:33)->(G:10)":57,"(C:34)->(G:02)":30},"sample":"13a_F730603","score":431,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggactgggcggtgctttatcccctt
>HELIUM_000100422_612GNAAXX:7:60:15952:8444#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{},"sample":"26a_F040644","score":751,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:38:9395:21179#0/1_sub[28..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":64,"pairing_fast_score":0.721,"pairing_mismatches":{"(c:02)->(a:34)":66,"(c:02)->(g:34)":81,"(c:02)->(g:35)":74,"(c:34)->(a:02)":29,"(t:34)->(g:02)":35},"sample":"26a_F040644","score":185,"score_norm":0.922,"seq_a_single":44,"seq_ab_match":59,"seq_b_single":44}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcacag
cctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:54:11106:5726#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatattcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:116:11081:4966#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":63,"pairing_fast_score":0.783,"pairing_mismatches":{"(a:10)->(t:02)":47,"(a:12)->(g:02)":36},"sample":"26a_F040644","score":748,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatattcgccagagaactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:90:18782:17335#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(C:07)->(G:34)":56,"(T:28)->(G:07)":39},"sample":"13a_F730603","score":2944,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggagttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:45:1647:13458#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":63,"pairing_fast_score":0.717,"pairing_mismatches":{"(C:02)->(T:23)":25,"(T:02)->(G:13)":53},"sample":"26a_F040644","score":259,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacgagcaaca
gcctgaaactcaaaggacttggcggtgctttacagccct
>HELIUM_000100422_612GNAAXX:7:80:14847:17414#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{},"sample":"29a_F260619","score":976,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagtgaactactagcaaca
gattaaacctcaaaggacttggcagtgctttatacccct
>HELIUM_000100422_612GNAAXX:7:47:15378:20254#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"sample":"29a_F260619","score":9,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggactgggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:43:9180:5216#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(a:02)->(c:31)":69,"(a:09)->(t:02)":47,"(a:11)->(t:02)":38},"sample":"26a_F040644","score":376,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtacgccagagaactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:70:2429:19509#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgccttacgtccct
>HELIUM_000100422_612GNAAXX:7:82:19039:16095#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(c:02)->(a:30)":67,"(c:02)->(t:28)":65,"(c:17)->(g:10)":52,"(t:02)->(g:15)":71,"(t:14)->(g:02)":38},"sample":"29a_F260619","score":522,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattactataacaaaattattcgccagagtactcccggcaat
agcttaaaacgcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:19:2965:12005#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":39,"pairing_fast_overlap":62,"pairing_fast_score":0.661,"pairing_mismatches":{"(C:02)->(A:34)":67,"(C:02)->(G:08)":43,"(C:02)->(G:28)":56,"(C:02)->(T:33)":65,"(T:27)->(G:02)":21},"sample":"13a_F730603","score":182,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgcgagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:7101:4690#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggacttctagcaata
gcttaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:116:4343:21191#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":4,"experiment":"wolf_diet","merged_sample":{"26a_F040644":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaatacacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:108:6006:8209#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":15,"experiment":"wolf_diet","merged_sample":{"26a_F040644":15},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtccgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:112:1653:17039#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:28)->(T:02)":27,"(C:02)->(A:34)":59,"(C:31)->(A:02)":26,"(T:21)->(G:23)":39},"sample":"13a_F730603","score":1071,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactatgcgccagagtactaccggcaat
agcttaaaactcaaaggactgggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:20:13935:18279#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":4,"experiment":"wolf_diet","merged_sample":{"15a_F730814":3,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacataagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:47:2051:17768#0/1_sub[28..127] {"ali_dir":"left","count":7,"experiment":"wolf_diet","merged_sample":{"13a_F730603":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"sample":"13a_F730603"}
ctagccttaagcacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:112:15920:4211#0/1_sub[28..127] {"ali_dir":"left","count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"sample":"13a_F730603","seq_ab_match":61}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggtaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:65:1843:2567#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":7,"experiment":"wolf_diet","merged_sample":{"26a_F040644":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaaccattctataacaaaataattcgccagagaactactagcaaca
gcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:84:18997:17126#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3247,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacaaaaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:28:9110:9657#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":5,"experiment":"wolf_diet","merged_sample":{"26a_F040644":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaccaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:68:2078:5357#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(a:09)->(t:02)":48,"(a:15)->(t:02)":39,"(a:23)->(g:02)":49,"(c:23)->(a:02)":42,"(c:25)->(t:02)":65},"sample":"15a_F730814","score":583,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatacgccagagaactaccggcaat
agctcaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:98:16660:15650#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":7,"experiment":"wolf_diet","merged_sample":{"15a_F730814":4,"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagaatactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:82:15344:16512#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactaktcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:41:4732:19512#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":28,"pairing_fast_overlap":62,"pairing_fast_score":0.475,"pairing_mismatches":{"(A:33)->(C:02)":25,"(A:34)->(C:02)":23,"(C:34)->(A:02)":30,"(C:34)->(T:02)":50,"(G:31)->(T:02)":55,"(T:29)->(G:02)":35,"(T:31)->(A:02)":22,"(T:31)->(C:02)":39},"sample":"29a_F260619","score":69,"score_norm":0.823,"seq_a_single":46,"seq_ab_match":51,"seq_b_single":46}
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccttt
>HELIUM_000100422_612GNAAXX:7:1:17413:9115#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(A:09)->(C:34)":71,"(A:16)->(C:31)":29,"(T:13)->(A:34)":25},"sample":"15a_F730814","score":3289,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
tagccctaaacacaaggaattaatataacaaaattattcgccagagtactaccggcaata
gcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:91:18617:6752#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":63,"pairing_fast_score":0.667,"pairing_mismatches":{"(A:02)->(C:06)":51,"(C:02)->(A:31)":67,"(C:35)->(A:02)":22,"(T:02)->(G:15)":53},"sample":"26a_F040644","score":537,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactccgagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:92:1339:19811#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"29a_F260619":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattacacaaacaaaattgttcacaagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:7868:9939#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"sample":"13a_F730603","score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactgcctgcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:116:6865:16299#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactrctagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:52:11854:5937#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(A:31)->(T:02)":39},"sample":"15a_F730814","score":8,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatacgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttattccctt
>HELIUM_000100422_612GNAAXX:7:33:7375:16412#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggagttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:15:13855:1746#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgcgaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:98:2014:13659#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(A:02)->(C:07)":72,"(A:02)->(C:22)":54,"(T:02)->(G:10)":71},"sample":"29a_F260619","score":1299,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccgccaat
agcttaaaacgcaaagagcttggcggtggtttataccctt
>HELIUM_000100422_612GNAAXX:7:71:15112:10075#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":38,"pairing_fast_overlap":62,"pairing_fast_score":0.644,"pairing_mismatches":{"(a:02)->(g:32)":77,"(a:26)->(g:02)":58,"(a:28)->(g:02)":32,"(g:02)->(t:22)":81,"(g:10)->(c:02)":54,"(g:27)->(c:02)":55,"(t:31)->(c:02)":38},"sample":"15a_F730814","score":174,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacgggcaat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:84:19566:16896#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:02)->(G:18)":61,"(A:24)->(G:25)":44,"(C:02)->(T:28)":65},"sample":"13a_F730603","score":1894,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccggagtactaccggcaat
ggcttaaaactcaaatgacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:73:13206:8766#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":3362,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacatttaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:23:13832:15380#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":13,"experiment":"wolf_diet","merged_sample":{"15a_F730814":9,"29a_F260619":4},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattagtcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:8:6794:4925#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":34,"experiment":"wolf_diet","merged_sample":{"15a_F730814":23,"29a_F260619":11},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:29:12399:3496#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":7,"experiment":"wolf_diet","merged_sample":{"26a_F040644":7},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccccaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:109:5071:7721#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":63,"pairing_fast_score":0.733,"pairing_mismatches":{"(A:02)->(G:17)":58,"(C:02)->(A:29)":68},"sample":"26a_F040644","score":1758,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactgctagcagca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:51:15223:18925#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctgccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:7:6024:9553#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(A:02)->(T:07)":51},"sample":"26a_F040644","score":1843,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacagtcaataaacaaggatgttcgccagagtacttctagcaatg
gcctaaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:25:6961:20785#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtcattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:67:1346:8868#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaacggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:15:7779:5773#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttatacactt
>HELIUM_000100422_612GNAAXX:7:9:6752:3403#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":63,"pairing_fast_score":0.917,"pairing_mismatches":{"(n:02)->(a:34)":72},"sample":"26a_F040644","score":2679,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccggagaactactagcaaca
gcttaaaactcaaaggacttggcggtgccttacgtccct
>HELIUM_000100422_612GNAAXX:7:100:3949:12906#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(T:34)->(G:02)":22},"sample":"13a_F730603","score":524,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaatagttatgtaaacaaaactattcgccagagtactaccagcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:21:1346:16481#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(c:02)->(a:29)":74,"(c:08)->(a:34)":59,"(c:11)->(t:02)":48,"(g:27)->(t:02)":47,"(t:10)->(g:02)":35},"sample":"29a_F260619","score":819,"score_norm":0.871,"seq_a_single":46,"seq_ab_match":54,"seq_b_single":46}
ttagccctaaacacaagtaattactataacaaaattattcgccagagcactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:13:16546:6705#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(a:17)->(c:02)":54,"(a:32)->(g:02)":31,"(c:10)->(t:02)":65,"(c:27)->(t:31)":81,"(c:28)->(g:02)":42},"sample":"29a_F260619","score":461,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacaggcaat
agctcaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:37:3162:20938#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(a:02)->(g:34)":77,"(a:02)->(t:34)":65,"(c:02)->(a:34)":74},"sample":"29a_F260619","score":1472,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacacataattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:13:7378:13716#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"sample":"26a_F040644","score":1858,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacaaccct
>HELIUM_000100422_612GNAAXX:7:54:4881:17230#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"13a_F730603","score":3704,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaatactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:101:5253:19534#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:33)->(T:02)":23,"(T:02)->(G:30)":39,"(T:32)->(G:19)":36,"(T:34)->(A:02)":21},"sample":"15a_F730814","score":381,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattatgcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctctataccctt
>HELIUM_000100422_612GNAAXX:7:89:15768:3751#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(A:02)->(G:24)":58,"(A:32)->(G:02)":37,"(T:33)->(G:02)":21},"sample":"13a_F730603","score":377,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcgat
agcttaaaactcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:101:16529:3822#0/1_sub[28..127] {"ali_dir":"left","count":7,"experiment":"wolf_diet","merged_sample":{"15a_F730814":5,"29a_F260619":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_b_single":46}
ttagccctaaacacaagtaataaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:89:8672:16537#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":63,"pairing_fast_score":0.683,"pairing_mismatches":{"(a:07)->(c:02)":39,"(a:28)->(g:02)":33,"(a:30)->(g:02)":51,"(a:31)->(g:02)":30,"(a:34)->(g:13)":72,"(c:19)->(a:02)":31},"sample":"26a_F040644","score":1105,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacacgaatgttagccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:8:13356:1359#0/1_sub[28..125] {"ali_dir":"left","ali_length":64,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":64,"pairing_fast_score":0.934,"pairing_mismatches":{"(a:02)->(g:34)":76},"sample":"26a_F040644","score":2909,"score_norm":0.984,"seq_a_single":44,"seq_ab_match":63,"seq_b_single":44}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatcct
>HELIUM_000100422_612GNAAXX:7:85:18012:12866#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":15,"experiment":"wolf_diet","merged_sample":{"26a_F040644":15},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:7:7092:11003#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":2,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"15a_F730814","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtacgtccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:111:4024:17034#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(a:06)->(c:06)":70,"(c:09)->(a:34)":58,"(t:35)->(g:32)":37},"sample":"26a_F040644","score":2773,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaacmcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:47:7078:13378#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(a:02)->(t:31)":47,"(c:32)->(a:07)":41},"sample":"26a_F040644","score":1224,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgcttttcatccct
>HELIUM_000100422_612GNAAXX:7:20:3082:8713#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":15,"experiment":"wolf_diet","merged_sample":{"26a_F040644":15},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcgaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:17:5366:13068#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"13a_F730603":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"13a_F730603","seq_a_single":46,"seq_b_single":46}
ctggccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:30:11039:18074#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":3,"experiment":"wolf_diet","merged_sample":{"15a_F730814":2,"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"seq_a_single":46,"seq_b_single":46}
ttagccctaaacccaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:2:19576:14210#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"26a_F040644":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgcttttcatccct
>HELIUM_000100422_612GNAAXX:7:54:14152:11458#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":63,"pairing_fast_score":0.783,"pairing_mismatches":{"(a:10)->(c:28)":69,"(c:15)->(a:33)":58},"sample":"29a_F260619","score":885,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactactagcaaca
gattaaacctcaaaggacttggcagtgccttatacccct
>HELIUM_000100422_612GNAAXX:7:32:4617:8780#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"sample":"15a_F730814","score":4051,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaagcacaagtaattaatataacaaaattattcgccagaggactactagcaat
agcttaaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:38:3580:6849#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":5,"experiment":"wolf_diet","merged_sample":{"29a_F260619":5},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":62,"sample":"29a_F260619","seq_a_single":46,"seq_b_single":46}
ttagccctaaacacaagtaattacacaaacaaaattgttcaccagagtactagcggcaac
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:110:1548:8097#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"15a_F730814":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaagtag","obimultiplex_reverse_tag":"gaagtag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":34,"pairing_fast_overlap":62,"pairing_fast_score":0.576,"pairing_mismatches":{"(A:02)->(G:06)":69,"(A:28)->(G:16)":34,"(A:28)->(G:22)":52,"(C:02)->(T:26)":65,"(C:10)->(T:27)":39},"sample":"15a_F730814","score":915,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacgcaagtaattaatataacaaaattattcgccagagtactaccggcgat
agcttaaagctcaaaggacttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:1:4197:12691#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":63,"pairing_fast_score":0.75,"pairing_mismatches":{"(a:02)->(g:34)":76,"(a:07)->(c:02)":63,"(c:02)->(a:34)":73,"(c:02)->(t:34)":80},"sample":"26a_F040644","score":173,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactaatagcaaca
gcatgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:6:2504:16102#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":3,"experiment":"wolf_diet","merged_sample":{"26a_F040644":3},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"26a_F040644","seq_a_single":45,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttctccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttacatccct
>HELIUM_000100422_612GNAAXX:7:74:7750:4627#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":1,"experiment":"wolf_diet","merged_sample":{"26a_F040644":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(G:34)->(A:02)":27},"sample":"26a_F040644","score":1260,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaatagacaagaatgttcgccagagtactactagcaaca
gcctgaaactcaaaggacttggcggtgctttatatccct
>HELIUM_000100422_612GNAAXX:7:84:17705:20546#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"count":2,"experiment":"wolf_diet","merged_sample":{"13a_F730603":2},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_overlap":63,"sample":"13a_F730603","seq_a_single":45,"seq_b_single":45}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat
agcttaaaactcaaaggacttgcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:94:13185:14593#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(c:02)->(a:33)":74,"(c:18)->(t:34)":48},"sample":"29a_F260619","score":1390,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattactcgccagagtactaccggcaat
agcttaaaactcaaaggacttggcggtgccttataccctt
>HELIUM_000100422_612GNAAXX:7:105:18163:20157#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"13a_F730603":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aattaac","obimultiplex_forward_tag":"aattaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(G:06)":52,"(C:02)->(G:10)":50,"(G:02)->(T:07)":62,"(G:34)->(A:17)":20},"sample":"13a_F730603","score":959,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtagtgccggcaat
atcttaaaactcaaaggatttggcggtgctttatgccctt
>HELIUM_000100422_612GNAAXX:7:21:9140:21312#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:26)->(N:04)":52,"(A:28)->(N:04)":49,"(C:21)->(A:32)":59,"(C:27)->(G:15)":56,"(C:28)->(N:04)":50,"(C:29)->(N:05)":53,"(T:26)->(N:04)":51},"sample":"29a_F260619","score":1466,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcaccagagtactaccgccaat
agcttaaaactcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:92:5445:7024#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(A:02)->(C:33)":54},"sample":"29a_F260619","score":979,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaagcacaagtaattaatataacaaaattattcgccagagtactaccggcaat
agcttaaagctcaaaggacttggcggtgctttataccctt
>HELIUM_000100422_612GNAAXX:7:3:3008:16359#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"count":1,"experiment":"wolf_diet","merged_sample":{"29a_F260619":1},"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(a:15)->(c:33)":51,"(c:23)->(a:24)":42,"(t:32)->(g:07)":38},"sample":"29a_F260619","score":3422,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgacagagtaccaccggcaat
agcttaaaactcaaaggacttggcggtgctttataccctt